Kinetics of protein adsorption/desorption mediated by pH-responsive polymer layer

doi:10.1088/1674-1056/24/11/113601

Abstract We propose a new way of regulating protein adsorption by using a pH-responsive polymer. According to the theoretical results obtained from the molecular theory and kinetic approaches, both thermodynamics and kinetics of protein adsorption are verified to be well controlled by the solution pH. The kinetics and the amount of adsorbed proteins at equilibrium are greatly increased when the solution environment changes from acid to neutral. The reason is that the increased pH promotes the dissociation of the weak polyelectrolyte, resulting in more charged monomers and more stretched chains. Thus the steric repulsion within the polymer layer is weakened, which effectively lowers the barrier felt by the protein during the process of adsorption. Interestingly, we also find that the kinetics of protein desorption is almost unchanged with the variation of pH. It is because although the barrier formed by the polymer layer changes along with the change of pH, the potential at contact with the surface varies equally. Our results may provide useful insights into controllable protein adsorption/desorption in practical applications.

Keywords: dissociation potential of mean-force barrier steric repulsion

Received: 12 March 2015
Revised: 01 July 2015
Accepted manuscript online:

PACS:	36.20.-r	(Macromolecules and polymer molecules)
	36.20.Ey	(Conformation (statistics and dynamics))
	68.47.Mn	(Polymer surfaces)
	82.35.Rs	(Polyelectrolytes)

Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 21274062, 11474155, and 91027040).

Corresponding Authors: Ren Chun-Lai
E-mail: chunlair@nju.edu.cn

Cite this article:

Su Xiao-Hang (苏晓航), Lei Qun-Li (雷群利), Ren Chun-Lai (任春来) Kinetics of protein adsorption/desorption mediated by pH-responsive polymer layer 2015 Chin. Phys. B 24 113601

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Kinetics of protein adsorption/desorption mediated by pH-responsive polymer layer

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