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Kinetics of protein adsorption/desorption mediated by pH-responsive polymer layer |
Su Xiao-Hang (苏晓航), Lei Qun-Li (雷群利), Ren Chun-Lai (任春来) |
National Laboratory of Solid State Microstructures and Department of Physics, Nanjing University, Nanjing 210093, China |
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Abstract We propose a new way of regulating protein adsorption by using a pH-responsive polymer. According to the theoretical results obtained from the molecular theory and kinetic approaches, both thermodynamics and kinetics of protein adsorption are verified to be well controlled by the solution pH. The kinetics and the amount of adsorbed proteins at equilibrium are greatly increased when the solution environment changes from acid to neutral. The reason is that the increased pH promotes the dissociation of the weak polyelectrolyte, resulting in more charged monomers and more stretched chains. Thus the steric repulsion within the polymer layer is weakened, which effectively lowers the barrier felt by the protein during the process of adsorption. Interestingly, we also find that the kinetics of protein desorption is almost unchanged with the variation of pH. It is because although the barrier formed by the polymer layer changes along with the change of pH, the potential at contact with the surface varies equally. Our results may provide useful insights into controllable protein adsorption/desorption in practical applications.
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Received: 12 March 2015
Revised: 01 July 2015
Accepted manuscript online:
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PACS:
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36.20.-r
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(Macromolecules and polymer molecules)
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36.20.Ey
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(Conformation (statistics and dynamics))
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68.47.Mn
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(Polymer surfaces)
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82.35.Rs
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(Polyelectrolytes)
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Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 21274062, 11474155, and 91027040). |
Corresponding Authors:
Ren Chun-Lai
E-mail: chunlair@nju.edu.cn
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Cite this article:
Su Xiao-Hang (苏晓航), Lei Qun-Li (雷群利), Ren Chun-Lai (任春来) Kinetics of protein adsorption/desorption mediated by pH-responsive polymer layer 2015 Chin. Phys. B 24 113601
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[1] |
Mulheran P and Kubiak K;2009 Mol. Simul. 35 561
|
[2] |
Alves N M, Pashkuleva I, Reis R L and Mano J F;2010 Small 6 2208
|
[3] |
Xue C Y, Choi B C, Choi S, Braun P V and Leckband D E;2012 Adv. Funct. Mater. 22 2394
|
[4] |
Wang K F, Zhou C C, Hong Y L and Zhang X D;2012 Interface Focus 2 259
|
[5] |
Wei Q, Becherer T, Angioletti-Uberti S, Dzubiella J, Wischke C, Neffe A T, Lendlein A, Ballauff M and Haag R;2014 Angew. Chem. Int. Ed. 53 8004
|
[6] |
Oberle M, Yigit C, Angioletti-Uberti S, Dzubiella J and Ballauff M;2015 J. Phys. Chem. B 119 3250
|
[7] |
Hlady V and Buijs J;1996 Curr. Opin. Biotechnol. 7 72
|
[8] |
Gray J J;2004 Curr. Opin. Struct. Biol. 14 110
|
[9] |
Halperin A and Kroeger M;2009 Langmuir 25 11621
|
[10] |
Cole M A, Voelcker N H, Thissen H and Griesser H J;2009 Biomaterials 30 1827
|
[11] |
Rabe M, Verdes D and Seeger S;2011 Adv. Colloid Interface Sci. 162 87
|
[12] |
Johansson C, Gernandt J, Bradley M, Vincent B and Hansson P;2010 J. Colloid Interface Sci. 347 241
|
[13] |
Zhang Y, Chan H F and Leong K W;2013 Adv. Drug Delivery Rev. 65 104
|
[14] |
Wu J, Kamaly N, Shi J J, Zhao L L, Xiao Z Y, Hollett G, John R, Ray S, Xu X Y, Zhang X Q, Kantoff P W and Farokhzad O C;2014 Angew. Chem. Int. Ed. 53 8975
|
[15] |
Chu X, Yu J and Hou Y L;2015 Chin. Phys. B 24 014704
|
[16] |
Ren C L and Ma Y Q;2011 Soft Matter 7 10841
|
[17] |
Ballauff M and Borisov O;2006 Curr. Opin. Colloid Interface Sci. 11 316
|
[18] |
Kumar A, Srivastava A, Galaev I Y and Mattiasson B;2007 Prog. Polym. Sci. 32 1205
|
[19] |
Krishnamoorthy M, Hakobyan S, Ramstedt M and Gautrot J E;2014 Chem. Rev. 114 10976
|
[20] |
Kost J and Langer R;2001 Adv. Drug Delivery Rev. 46 125
|
[21] |
Huber D L, Manginell R P, Samara M A, Kim B I and Bunker B C;2003 Science 301 352
|
[22] |
Minko S;2006 Polym. Rev. 46 397
|
[23] |
Nap R, Gong P and Szleifer I;2006 J. Polym. Sci. B: Polym. Phys. 44 2638
|
[24] |
Schmaljohann D;2006 Adv. Drug Delivery Rev. 58 1655
|
[25] |
Halperin A;1999 Langmuir 15 2525
|
[26] |
Tong Z Y, Zhu Y J and Tong C H;2014 Chin. Phys. B 23 038202
|
[27] |
Xu Y L, Chen X Q, Chen H Y, Xu S H, Liu H L and Hu Y;2012 Mol. Simul. 38 274
|
[28] |
Jonsson M and Johansson H O;2004 Colloids Surf. B 37 71
|
[29] |
Fang F and Szleifer I;2001 Biophys. J. 80 2568
|
[30] |
Fang F, Satulovsky J and Szleifer I;2005 Biophys. J. 89 1516
|
[31] |
Bonincontro A, De Francesco A and OnoriG 1998 Colloids Surf. B 12 1
|
[32] |
Kuehner D E, Engmann J, Fergg F, Wernick M, Blanch H W and Prausnitz J M;1999 J. Phys. Chem. B 103 1368
|
[33] |
Fang F and Szleifer I;2003 J. Chem. Phys. 119 1053
|
[34] |
Szleifer I;1997 Biophys. J. 72 595
|
[35] |
Lee S J and Park K;1994 J. Vac. Sci. Technol. A 12 2949
|
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