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Chin. Phys. B, 2008, Vol. 17(6): 2288-2291    DOI: 10.1088/1674-1056/17/6/059
CROSS DISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY Prev   Next  

Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents

Zhang Hong-Yan(张洪艳)a)b), Lu Heng(陆珩)a), Li Wei(李巍)c), Liang Ru-Qiang(梁汝强)c), Jin Kui-Juan(金奎娟)a), Zhou Yue-Liang(周岳亮)a), Ruan Kang-Cheng (阮康成)c), and Yang Guo-Zhen(杨国桢)a)
a Laboratory of Optical Physics, Institute of Physics and Center for Condensed Matter Physics, Chinese Academy of Sciences, Beijing 100190, China; b Laboratory of Organic Optoelectronic Functional Materials and Molecular Engineering, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China; c Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Abstract  This paper reports that the detection to the protein in microarray format is carried out by oblique-incidence reflectivity difference (OI--RD) analysis without any labelling agents. The OI--RD intensities not only depend on the protein structure, but also vary with the protein concentration. The results indicate that this method should have potential application in detection of biochemical processes. The high throughout and in situ detection can be achieved by this method with further improving of the experimental system.
Keywords:  detection      OI--RD      protein      microarray  
Received:  15 October 2007      Revised:  30 October 2007      Accepted manuscript online: 
PACS:  87.14.E- (Proteins)  
  87.15.R- (Reactions and kinetics)  
  87.80.-y (Biophysical techniques (research methods))  
Fund: Project supported by the Chinese Academy of Sciences for Key Topics in Innovation Engineering (Grant No L3FX051W).

Cite this article: 

Zhang Hong-Yan(张洪艳), Lu Heng(陆珩), Li Wei(李巍), Liang Ru-Qiang(梁汝强), Jin Kui-Juan(金奎娟), Zhou Yue-Liang(周岳亮), Ruan Kang-Cheng (阮康成), and Yang Guo-Zhen(杨国桢) Detection of microarray protein biomolecules by oblique-incidence reflectivity difference technique without labelling agents 2008 Chin. Phys. B 17 2288

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