1. School of Science, International Joint Research Center for Nanophotonics and Biophotonics, Changchun University of Science and Technology, 7089 Wei-Xing Road, Changchun 130022, China; 2. Key Laboratory for Photonic and Electric Bandgap Materials, Ministry of Education, Harbin Normal University, Harbin 150025, China
Abstract Biofunctional europium (Ⅲ)-doped ZnS (ZnS:Eu) nanocrystals are prepared by a sol-gel method. The characteristic luminescence of ZnS:Eu is used as a probe signal to realize sensitive immunoassay. The luminescence intensity of the Eu3+ in the ZnS matrix shows strong concentration dependence, and the optimal doping concentration is 4%. However, the emission wavelengths of the ZnS:Eu nanocrystals are not dependent on doping concentration nor the temperature (from 100 K to 300 K). Our results show that these features allow for reliable immunoassay. Human immunoglobulin, used as a target analyte, is captured by antibody modified ZnS:Eu probe and is finally enriched on gold substrate for detection. High specificity of the assay is demonstrated by control experiments. The linear detection range is 10 nM-800 nM, and the detection limit is about 9.6 nM.
Fund: Project supported by the National Natural Science Foundation of China (Grant No. 61205193), the Project of Science and Technology of Jilin Province, China (Grant No. 20140520107JH), the Technology Foundation of Jilin Provincial Department of Human Resources and Social Security, China (Grant No. RL201306), and the Science Foundation for Young Scientists of Changchun University of Science and Technology, China (Grant No. XQNJJ-2015-03).
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