INTERDISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY |
Prev
Next
|
|
|
Initial conformation of kinesin’s neck linker |
Geng Yi-Zhao (耿轶钊)a, Ji Qing (纪青)b, Liu Shu-Xia (刘书霞)b, Yan Shi-Wei (晏世伟)a c |
a College of Nuclear Science and Technology, Beijing Normal University, Beijing 100875, China;
b Institute of Biophysics, Hebei University of Technology, Tianjin 300401, China;
c Beijing Radiation Center, Beijing 100875, China |
|
|
Abstract How ATP binding initiates the docking process of kinesin's neck linker is a key question in understanding kinesin mechanisms. By exploiting a molecular dynamics method, we investigate the initial conformation of kinesin's neck linker in its docking process. We find that, in the initial conformation, the neck linker has interactions with β0 and forms a 'cover-neck bundle' structure with β0. From this initial structure, the formation of extra turns and the docking of the cover-neck bundle structure can be achieved. The motor head provides a forward force on the initial cover-neck bundle structure through ATP-induced rotation. This force, together with the hydrophobic interaction of ILE327 with the hydrophobic pocket on the motor head, drives the formation of the extra turn and initiates the neck linker docking process. Based on these findings, a pathway from ATP binding-induced motor head rotation to neck linker docking is proposed.
|
Received: 12 April 2014
Revised: 20 May 2014
Accepted manuscript online:
|
PACS:
|
87.16.Nn
|
(Motor proteins (myosin, kinesin dynein))
|
|
87.10.Tf
|
(Molecular dynamics simulation)
|
|
87.15.hp
|
(Conformational changes)
|
|
Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 10975044 and 10975019), the Foundation of the Ministry of Personnel of China for Returned Scholars (Grant No. MOP2006138), the Fundamental Research Funds for the Central University, and the Key Subject Construction Project of Hebei Provincial Universities. |
Corresponding Authors:
Ji Qing
E-mail: jiqingch@hebut.edu.cn;yansw@bnu.edu.cn
|
About author: 87.16.Nn; 87.10.Tf; 87.15.hp |
Cite this article:
Geng Yi-Zhao (耿轶钊), Ji Qing (纪青), Liu Shu-Xia (刘书霞), Yan Shi-Wei (晏世伟) Initial conformation of kinesin’s neck linker 2014 Chin. Phys. B 23 108701
|
|
| [36] | Rosenfeld S S, Xing J, Jefferson G M, Cheung H C and King P H 2002 J. Biol. Chem. 277 36731
|
|
| [37] | Rosenfeld S S, Fordyce P M, Jefferson G M, King P H and Block S M 2003 J. Biol. Chem. 278 18550
|
|
| [38] | Uemura S and Ishiwata S 2003 Nat. Struct. Mol. Biol. 10 308
|
|
| [39] | Skiniotis G, Cochran J C, Müller J, Mandelkow E, Gilbert S P and Hoenger A 2004 EMBO J. 23 989
|
|
| [40] | Kikkawa M and Hirokawa N 2006 EMBO J. 25 4187
|
|
| [41] | Parke C L, Wojcik E J, Kim S and Worthylake D K 2010 J. Biol. Chem. 285 5859
|
|
| [1] | Courant R 1962 Methods of Mathematical Physics, Vol. 2 (New York: Interscience) pp. 183-187
|
|
| [2] | Huang S X and Wu R S 2009 Mathematical Physics Problems in Atmospheric Science, 3nd edn. (Beijing: Chinese Meteorological Press) pp. 413-414 (in Chinese)
|
|
| [3] | Tosaka N, Onishi K and Yamamato M 1999 Mathematical Approach and Solution Methods for Inverse Problems: Inverse Analysis of Partial Differential Equations (Tokyo: University of Tokyo Press) pp. 117-168
|
|
| [4] | Kirsch A 1996 An Introduction to the Mathematic Theory of Inverse Problems (New York: Springer-Verlag) pp. 9-14
|
|
| [42] | Parker D, Bryant Z and Delp S L 2009 Cell Mol. Bioeng. 2 366
|
No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
Altmetric
|
blogs
Facebook pages
Wikipedia page
Google+ users
|
Online attention
Altmetric calculates a score based on the online attention an article receives. Each coloured thread in the circle represents a different type of online attention. The number in the centre is the Altmetric score. Social media and mainstream news media are the main sources that calculate the score. Reference managers such as Mendeley are also tracked but do not contribute to the score. Older articles often score higher because they have had more time to get noticed. To account for this, Altmetric has included the context data for other articles of a similar age.
View more on Altmetrics
|
|
|