Real time detection of antibody–antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system
Zhang Hong-Yan(张洪艳)a), Yang Li-Quan(杨立泉)a), Meng Lan(孟岚)b), Nie Jia-Cai(聂家财)b)†, Ning Ting-Yin(宁廷银)a), Liu Wei-Min(刘卫敏)a), Sun Jia-Yu(孙嘉宇)a), and Wang Peng-Fei(汪鹏飞)a)‡
a. Key Laboratory of Photochemical Conversion and Optoelectronic Materials, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
b. Departments of Physics, Beijing Normal University, Beijing 100875, China
Abstract A laser scanning confocal imaging-surface plasmon resonance (LSCI-SPR) instrument integrated with a wavelength-dependent surface plasmon resonance (SPR) sensor and a laser scanning confocal microscopy (LSCM) is built to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibodies in real time. The shifts of resonant wavelength at different reaction time stages are obtained by SPR, corresponding well with the changes of the fluorescence intensity collected by using LSCM. The instrument shows the merits of the combination and complementation of the SPR and LSCM, with such advantages as quantificational analysis, high spatial resolution and real time monitor, which are of great importance for practical applications in biosensor and life science.
Received: 22 July 2011
Revised: 13 August 2011
Accepted manuscript online:
PACS:
06.90.+v
(Other topics in metrology, measurements, and laboratory procedures)
Fund: Project supported by the Instrument Developing Project of the Chinese Academy of Sciences (Grant No. YZ200740), the National Natural Science Foundation of China (Grant Nos. 60978034 and 10974019), and the National High Technology Research and Development Program of China (Grant No. 2009AA03Z318).
Corresponding Authors:
Nie Jia-Cai,jcnie@bnu.eud.cn;Wang Peng-Fei,wangpf@mail.ipc.ac.cn
E-mail: jcnie@bnu.eud.cn;wangpf@mail.ipc.ac.cn
Cite this article:
Zhang Hong-Yan(张洪艳), Yang Li-Quan(杨立泉), Meng Lan(孟岚), Nie Jia-Cai(聂家财), Ning Ting-Yin(宁廷银), Liu Wei-Min(刘卫敏), Sun Jia-Yu(孙嘉宇), and Wang Peng-Fei(汪鹏飞) Real time detection of antibody–antigen interaction using a laser scanning confocal imaging-surface plasmon resonance system 2012 Chin. Phys. B 21 020601
Enhancement of electron-ion recombination rates at low energy range in the heavy ion storage ring CSRm Nadir Khan, Zhong-Kui Huang(黄忠魁), Wei-Qiang Wen(汶伟强), Shu-Xing Wang(汪书兴), Han-Bing Wang(汪寒冰), Wan-Lu Ma(马万路), Xiao-Long Zhu(朱小龙), Dong-Mei Zhao(赵冬梅), Li-Jun Mao(冒立军), Jie Li(李杰), Xiao-Ming Ma(马晓明), Mei-Tang Tang(汤梅堂), Da-Yu Yin(殷达钰), Wei-Qing Yang(杨维青), Jian-Cheng Yang(杨建成), You-Jin Yuan(原有进), Lin-Fan Zhu(朱林繁), Xin-Wen Ma(马新文). Chin. Phys. B, 2020, 29(3): 033401.
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