Analysis of second-harmonic generation microscopy under refractive index mismatch
Wang Xiang-Hui(王湘晖)a)b)†, Lin Lie(林列)a)b), and Zhang Yang(张杨)a)b)
a The Key Laboratory of Opto-electronic Information Science and Technology, the Ministry of Education, Tianjin 300071, China; b Institute of Modern Optics, Nankai University, Tianjin 300071, China
Abstract On the basis of the vector diffraction theory and Green’s function method, this paper investigates the effects of refractive index mismatch on second-harmonic generation (SHG) microscopy. The polarization distribution and SHG intensity are calculated as functions of the sample radius and probe depth. The numerical results show that refractive index mismatch can result in peak intensity degradation, increase secondary lobes and extension of secondharmonic polarization distribution. Because of the attenuation of polarization intensity, the detected SHG intensity significantly decreases with increasing probe depth, which can limit the imaging depth of SHG microscopy inside thick samples. Forward SHG intensity decays slowly than backward SHG, due to the combination of extension secondharmonic polarization distribution and strong dependency of forward SHG on sample radius.
Accepted manuscript online:
PACS:
42.65.Ky
(Frequency conversion; harmonic generation, including higher-order harmonic generation)
Wang Xiang-Hui(王湘晖), Lin Lie(林列), and Zhang Yang(张杨) Analysis of second-harmonic generation microscopy under refractive index mismatch 2007 Chinese Physics 16 3285
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