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Patterned line-illumination mesoscopy with a moving slit for enhancing background suppression in cortex-wide mouse brain imaging |
| Chaowei Zhuang(庄超玮)1,†, Yi Yang(杨懿)1,2,3,4, and Hao Xie(谢浩)5,6,‡ |
1 Zhejiang Hehu Technology Co., Ltd., Hangzhou 311121, China;
2 School of Reliability and Systems Engineering, Beihang University, Beijing 100191, China;
3 Peng Cheng Laboratory, Shenzhen 518000, China;
4 Hangzhou Innovation Institute, Beihang University, Hangzhou 310052, China;
5 Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
6 Yangtze River Delta Physics Research Center Co., Ltd., Liyang 213300, China |
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Abstract Wide-field mesoscopy provides the capabilities of cortex-wide field of view (FOV), cellular resolution and high frame rate for neuronal imaging in the mouse brain. However, inherent background fluorescence degrades the image quality and hinders neuronal signal extraction. To address this problem, we first introduce a cortex-wide, high-resolution line-illumination mesoscope with a moving slit designed for in vivo mouse brain imaging. This system achieves a 6.6$\times$6.6 mm FOV, microscale cellular resolution, a high frame rate of 10 Hz, as well as the background rejection ability. Furthermore, we integrated patterned illumination into the system to enhance the background suppression. Experimental results show that the proposed system successfully captures neurodynamics in the living mouse brain. Compared with conventional wide-field mesoscopes, the cortex-wide patterned line-illumination mesoscope (PLIM) achieves a threefold increase in the signal-to-background ratio (SBR). With patterned illumination integrated, the SBR enhancement further reaches four-and-a-half-fold.
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Received: 14 May 2025
Revised: 12 July 2025
Accepted manuscript online: 28 July 2025
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PACS:
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87.85.Pq
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(Biomedical imaging)
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87.19.lh
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(Optical imaging of neuronal activity)
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| Fund: H. Xie gratefully acknowledges the support from the National Natural Science Foundation of China (Grant No. 61971256). |
Corresponding Authors:
Chaowei Zhuang, Hao Xie
E-mail: zhuangchaowei@hehutek.com;xiehao@iphy.ac.cn
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Cite this article:
Chaowei Zhuang(庄超玮), Yi Yang(杨懿), and Hao Xie(谢浩) Patterned line-illumination mesoscopy with a moving slit for enhancing background suppression in cortex-wide mouse brain imaging 2026 Chin. Phys. B 35 028704
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