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Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy |
Zhang Kai (张凯)a, Li Dee (李德娥)b, Hong You-Li (洪友丽)a, Zhu Pei-Ping (朱佩平)a, Yuan Qing-Xi (袁清习)a, Huang Wan-Xia (黄万霞)a, Gao Kun (高昆)c, Zhou Hong-Zhang (周红章)b, Wu Zi-Yu (吴自玉)a c |
a Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China; b Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China; c National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230026, China |
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Abstract A penetrating view of the three-dimensional nano-structure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscope are quite promising for insect morphology studies, in particularly, provide an attractive alternative to destructive techniques for internal soft tissues.
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Received: 25 December 2012
Revised: 26 February 2013
Accepted manuscript online:
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PACS:
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68.37.Yz
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(X-ray microscopy)
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81.70.Tx
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(Computed tomography)
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07.85.Qe
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(Synchrotron radiation instrumentation)
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87.16.Mq
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(Morphology of nerve cells)
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Fund: Project supported by the National Basic Research Program of China (Grant Nos. 2012CB825800 and 2009CB930804), the Key Program of the National Natural Science Foundation of China (Grant Nos. 10734070 and 10774144), the National Natural Science Foundation of China (Grant Nos. 11205189, 10979055, 31071909, and 31272358), and the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KJCX2-YW-N42). |
Corresponding Authors:
Zhou Hong-Zhang, Wu Zi-Yu
E-mail: zhouhz@ioz.ac.cn;wuzy@ustc.edu.cn
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Cite this article:
Zhang Kai (张凯), Li Dee (李德娥), Hong You-Li (洪友丽), Zhu Pei-Ping (朱佩平), Yuan Qing-Xi (袁清习), Huang Wan-Xia (黄万霞), Gao Kun (高昆), Zhou Hong-Zhang (周红章), Wu Zi-Yu (吴自玉) Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy 2013 Chin. Phys. B 22 076801
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[1] |
Hennig W 1965 Annu. Rev. Entomol. 10 97
|
[2] |
Zhang K, Li D, Zhu P, Yuan Q, Huang W, Liu X, Hong Y, Gao G, Ge X and Zhou H 2010 Anal. Bioanal. Chem. 397 1
|
[3] |
Li D, Zhang K, Zhu P, Wu Z and Zhou H 2011 Anal. Bioanal. Chem. 401 1
|
[4] |
Betz O, Wegst U, Weide D, Heethoff M, Helfen L, Lee W K and Cloetens P 2007 J. Microsc. 227 51
|
[5] |
Sakdinawat A and Attwood D 2010 Nat. Photonics 12 840
|
[6] |
Kaulich B, Thibault P, Gianoncelli A and Kiskinova M 2011 J. Phys. Condens. Matter 23 083002
|
[7] |
Withers P 2007 Mater. Today 10 26
|
[8] |
Zhang X Z, Xu Z, Zhen X J, Wang Y, Guo Z, Yan R, Chang R, Zhou R R and Tai R Z 2010 Acta Phys. Sin. 59 4535 (in Chinese)
|
[9] |
Chen Y T, Chen T Y, Yi J, Chu Y S, Lee W K, Wang C L, Kempson I M, Hwu Y, Gajdosik V and Margaritondo G 2011 Opt. Lett. 36 1269
|
[10] |
Youn H S and Jung S W 2006 J. Microsc. 223 53
|
[11] |
Andrews J, Brennan S, Patty C, Luening K, Pianetta P, Almeida E, van der Meulen M, Feser M, Gelb J and Rudati J 2008 Synchrotron Radiat. News 21 17
|
[12] |
Larabell C A and Le Gros M A 2004 Mol. Biol. Cell 15 957
|
[13] |
Chen J, Wu C, Tian J, Li W, Yu S and Tian Y 2008 Appl. Phys. Lett. 92 233104
|
[14] |
Li W, Wang N, Chen J, Liu G, Pan Z, Guan Y, Yang Y, Wu W, Tian, J and Wei S 2009 Appl. Phys. Lett. 95 053108
|
[15] |
Yin G, Tang M, Song Y, Chen F, Liang K, Duewer F, Yun W, Ko C and Shieh H 2006 Appl. Phys. Lett. 88 241115
|
[16] |
Yuan Q, Zhang K, Hong Y, Huang W, Gao K, Wang Z, Zhu P, Gelb J, Tkachuk A and Hornberger B 2012 J. Synchrotron Rad. 19 1021
|
[17] |
Hoebeke E R 1985 J. N. Y. Entomol. Soc. 93 913
|
[18] |
Snodgrass R 1935 Principles of Insect Morphology McGraw-Hill Book Company (New York & London: McGraw-Hill Book Company)
|
[19] |
Klimaszewski J 1984 Mem. Entomol. Soc. Can. 116 3
|
[20] |
Gack C and Peschke K 1994 Zoomorphology 114 227
|
[21] |
Arnqvist G 1997 Biol. J. Linn. Soc. 60 365
|
[22] |
Eberhard W 2010 Genetica 138 5
|
[23] |
Jearanaikoon S and Abraham-Peskir J V 2005 J. Microsc. 218 185
|
[24] |
Wright R 2000 Microsc. Res. Tech. 51 496
|
[25] |
Yang Y, Li W, Liu G, Zhang X, Chen J, Wu W, Guan Y, Xiong Y, Tian Y and Wu Z 2010 J. Microsc. 240 14
|
[26] |
Chao W, Harteneck B, Liddle J, Anderson E and Attwood D 2005 Nature 435 1210
|
[27] |
Chen J, Yang Y, Zhang X, Andrews J C, Pianetta P, Guan Y, Liu G, Xiong Y, Wu Z and Tian Y 2010 Anal. Bioanal. Chem. 397 2117
|
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