Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy

doi:10.1088/1674-1056/22/7/076801

Abstract A penetrating view of the three-dimensional nano-structure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscope are quite promising for insect morphology studies, in particularly, provide an attractive alternative to destructive techniques for internal soft tissues.

Keywords: X-ray microscopy computed tomography synchrotron radiation source morphology

Received: 25 December 2012
Revised: 26 February 2013
Published: 01 June 2013

PACS:	68.37.Yz	(X-ray microscopy)
	81.70.Tx	(Computed tomography)
	07.85.Qe	(Synchrotron radiation instrumentation)
	87.16.Mq	(Morphology of nerve cells)

Fund: Project supported by the National Basic Research Program of China (Grant Nos. 2012CB825800 and 2009CB930804), the Key Program of the National Natural Science Foundation of China (Grant Nos. 10734070 and 10774144), the National Natural Science Foundation of China (Grant Nos. 11205189, 10979055, 31071909, and 31272358), and the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KJCX2-YW-N42).

Corresponding Authors: Zhou Hong-Zhang, Wu Zi-Yu
E-mail: zhouhz@ioz.ac.cn;wuzy@ustc.edu.cn

Cite this article:

Zhang Kai, Li Dee, Hong You-Li, Zhu Pei-Ping, Yuan Qing-Xi, Huang Wan-Xia, Gao Kun, Zhou Hong-Zhang, Wu Zi-Yu Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy 2013 Chin. Phys. B 22 076801

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