Helicase activity and substrate specificity of RecQ5β

doi:10.1088/1674-1056/26/6/068701

Abstract

RecQ5β is an essential DNA helicase in humans, playing important roles in DNA replication, repair, recombination and transcription. The unwinding activity and substrate specificity of RecQ5β is still elusive. Here, we used stopped-flow kinetic method to measure the unwinding and dissociation kinetics of RecQ5β with several kinds of DNA substrates, and found that RecQ5β could well unwind ss/dsDNA, forked DNA and Holiday junction, but was compromised in unwinding blunt DNA and G-quadruplex. Rec5β has the preferred unwinding specificity for certain DNA substrates containing the junction point, which may improve the binding affinity and unwinding activity of RecQ5β. Moreover, from a comparison with the truncated RecQ5β^1-467, we discovered that the C-terminal domain might strongly influence the unwinding activity and binding affinity of RecQ5β. These results may shed light on the physiological functions and working mechanisms of RecQ5β helicase.

Keywords: helicase RecQ5β DNA substrate specificity unwinding kinetics

Received: 11 March 2017
Revised: 10 April 2017
Accepted manuscript online:

PACS:	87.14.ej	(Enzymes)
	87.14.gk	(DNA)
	87.15.R-	(Reactions and kinetics)

Fund:

Project supported by the National Natural Science Foundation of China (Grant Nos. 11674383, 11474346, and 11274374), the National Basic Research Program of China (Grant No. 2013CB837200), and the National Key Research and Development Program of China (Grant No. 2016YFA0301500).

Corresponding Authors: Hui Li, Shuo-Xing Dou
E-mail: huili@iphy.ac.cn;sxdou@iphy.ac.cn

Cite this article:

Jing You(尤菁), Ya-Nan Xu(徐雅楠), Hui Li(李辉), Xi-Ming Lu(吕袭明), Wei Li(李伟), Peng-Ye Wang(王鹏业), Shuo-Xing Dou(窦硕星), Xu-Guang Xi(奚绪光) Helicase activity and substrate specificity of RecQ5β 2017 Chin. Phys. B 26 068701

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Helicase activity and substrate specificity of RecQ5β

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