Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice
Ren Zhao-Yu (任兆玉)a, Xu Xiao-Ming (许晓明)b, Wang Shui-Cai (王水才)c, Xin Yue-Yong (辛越勇)d, He Jun-Fang (贺俊芳)c, Hou Xun (侯洵)ac
a Institute of Photonics & Photo-Technology, Northwest University, Xi'an 710068, China; b Laboratory of Photosynthesis Research, Nanjing Agricultural University, Nanjing 210095, China; c State Key Laboratory of Transience Optics Technology, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710068, China; d Laboratory of Photosynthesis Basic Research, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
Abstract A high yielding rice variety mutant (Oryza sativa L., Zhenhui 249) with low chlorophyll b (Chl b) has been discovered in natural fields. It has a quality character controlled by a pair of recessive genes (nuclear gene). The partial loss of Chl b in content affects the efficiency of light harvest in a light harvest complex (LHC), thus producing the difference of the exciting energy transfer and the efficiency of photochemistry conversion between the mutant and wild-type rice in photosynthetic unit. The efficiency of utilizing light energy is higher in the mutant than that in the wild-type rice relatively. For further discussion of the above-mentioned difference and learning about the mechanism of the increase in the photochemical efficiency of the mutant, the pico-second resolution fluorescence spectrum measurement with delay-frame-scanning single photon counting technique is adopted. Thylakoid membranes of the mutant and the wild-type rice are excited by an Ar^+ laser with a pulse width of 120 ps, repetition rate of 4 MHz and wavelength of 514 nm. Compared with the time and spectrum property of exciting fluorescence, conclusions of those ultrafast dynamic experiments are: 1) The speeds of the exciting energy transferred in photo-system I are faster than that in photo-system II in both samples. 2) The speeds of the exciting energy transfer of mutant sample are faster than those of the wild-type. This might be one of the major reasons why the efficiency of photosynthesis is higher in mutant than that in the wild-type rice.
Received: 09 January 2003
Revised: 23 May 2003
Accepted manuscript online:
Fund: Project supported by the State Key Development Program for Basic Research of China (Grant No G1998010100).
Cite this article:
Ren Zhao-Yu (任兆玉), Xu Xiao-Ming (许晓明), Wang Shui-Cai (王水才), Xin Yue-Yong (辛越勇), He Jun-Fang (贺俊芳), Hou Xun (侯洵) Research on the ultrafast fluorescence property of thylakoid membranes of the wild-type and mutant rice 2003 Chinese Physics 12 1159
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