Structural biology revolution led by technical breakthroughs in cryo-electron microscopy

doi:10.1088/1674-1056/27/5/058703

Abstract Recent technical breakthroughs in cryo-electron microscopy (cryo-EM) revolutionized structural biology, which led to the 2017 Nobel Prize in chemistry being awarded to three scientists, Jacques Dubochet, Joachim Frank, and Richard Henderson, who made groundbreaking contributions to the development of cryo-EM. In this review, I will give a comprehensive review of the developmental history of cryo-EM, the technical aspects of the breakthrough in cryo-EM leading to the structural biology revolution, including electron microscopy, image recording devices and image processing algorithms, and the major scientific achievements by Chinese researchers employing cryo-EM, covering protein complexes involved in or related to gene expression and regulation, protein synthesis and degradation, membrane proteins, immunity, and viruses. Finally, I will give a perspective outlook on the development of cryo-EM in the future.

Keywords: cryo-electron microscopy (cryo-EM) structural biology protein molecular machinery

Received: 14 December 2017
Revised: 22 February 2018
Accepted manuscript online:

PACS:	87.80.-y	(Biophysical techniques (research methods))
	87.64.Ee	(Electron microscopy)
	87.15.B-	(Structure of biomolecules)

Fund: Project supported by the National Key Research and Development Program of China (Grant No.2017YFA0504700) and the National Natural Science Foundation of China (Grant Nos.31570732 and 31770785).

Corresponding Authors: Chang-Cheng Yin
E-mail: ccyin@hsc.pku.edu.cn

Cite this article:

Chang-Cheng Yin(尹长城) Structural biology revolution led by technical breakthroughs in cryo-electron microscopy 2018 Chin. Phys. B 27 058703

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