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Chin. Phys. B, 2011, Vol. 20(2): 024301    DOI: 10.1088/1674-1056/20/2/024301
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Correlation between microbubble-induced acoustic cavitation and hemolysis in vitro

Guo Xia-Shenga, Zhang Donga, Zhang Chun-Bingb, Liu Zhengc
a Institute of Acoustics, Key Laboratory of Modern Acoustics (Nanjing Universit25), Ministry of Education, Nanjing University, Nanjing 210093, China; b The Traditional Chinese Medicine Hospital of Jiangsu Province, Nanjing 210029, China; Institute of Acoustics, Key Laboratory of Modern Acoustics (Nanjing Universit25), Ministry of Education, Nanjing University, Nanjing 210093, China; c Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
Abstract  Microbubbles promise to enhance the efficiency of ultrasound-mediated drug delivery and gene therapy by taking advantage of artificial cavitation nuclei. The purpose of this study is to examine the ultrasound-induced hemolysis in the application of drug delivery in the presence of microbubbles. To achieve this goal, human red blood cells mixed with microbubbles were exposed to 1-MHz pulsed ultrasound. The hemolysis level was measured by a flow cytometry, and the cavitation dose was detected by a passive cavitation detecting system. The results demonstrate that larger cavitation dose would be generated with the increase of acoustic pressure, which might give rise to the enhancement of hemolysis. Besides the experimental observations, the acoustic pressure dependence of the radial oscillation of microbubble was theoretically estimated. The comparison between the experimental and calculation results indicates that the hemolysis should be highly correlated to the acoustic cavitation.
Keywords:  hemolysis      passive cavitation detection      microbubbles      acoustic cavitation  
Received:  17 June 2010      Revised:  12 July 2010      Published:  15 February 2011
PACS:  43.25.+y (Nonlinear acoustics)  
  43.35.+d (Ultrasonics, quantum acoustics, and physical effects of sound)  
  43.80.+p (Bioacoustics)  
Fund: Project supported by the National Basic Research Program of China (Grant No. 2010CB732600), the National Natural Science Foundation of China (Grant Nos. 10774071, 10974093, 10974098, and 30672014), the Natural Science Foundation of Jiangsu Province of China (Grant NO. BE2010768), and the Fund of the State Key Lab of Acoustics.

Cite this article: 

Zhang Chun-Bing, Liu Zheng, Guo Xia-Sheng, Zhang Dong Correlation between microbubble-induced acoustic cavitation and hemolysis in vitro 2011 Chin. Phys. B 20 024301

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