Please wait a minute...
Chin. Phys. B, 2016, Vol. 25(9): 090507    DOI: 10.1088/1674-1056/25/9/090507
SPECIAL TOPIC—Physical research in liquid crystal Prev   Next  

Analysis of the induction of the myelin basic protein binding to the plasma membrane phospholipid monolayer

Lei Zhang(张蕾), Changchun Hao(郝长春), Ying Feng(冯盈), Feng Gao(高峰), Xiaolong Lu(逯晓龙), Junhua Li(李俊花), Runguang Sun(孙润广)
School of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China
Abstract  Myelin basic protein (MBP) is an essential structure involved in the generation of central nervous system (CNS) myelin. Myelin shape has been described as liquid crystal structure of biological membrane. The interactions of MBP with monolayers of different lipid compositions are responsible for the multi-lamellar structure and stability of myelin. In this paper, we have designed MBP-incorporated model lipid monolayers and studied the phase behavior of MBP adsorbed on the plasma membrane at the air/water interface by thermodynamic method and atomic force microscopy (AFM). By analyzing the pressure-area (π-A) and pressure-time (π-T) isotherms, univariate linear regression equation was obtained. In addition, the elastic modulus, surface pressure increase, maximal insertion pressure, and synergy factor of monolayers were detected. These parameters can be used to modulate the monolayers binding of protein, and the results show that MBP has the strongest affinity for 1,2-dipalmitoyl-sn-glycero-3- phosphoserine (DPPS) monolayer, followed by DPPC/DPPS mixed and 1,2-dipalmitoyl-sn-glycero-3-phospho-choline (DPPC) monolayers via electrostatic and hydrophobic interactions. AFM images of DPPS and DPPC/DPPS mixed monolayers in the presence of MBP (5 nM) show a phase separation texture at the surface pressure of 20 mN/m and the incorporation of MBP put into the DPPC monolayers has exerted a significant effect on the domain structure. MBP is not an integral membrane protein but, due to its positive charge, interacts with the lipid head groups and stabilizes the membranes. The interaction between MBP and phospholipid membrane to determine the nervous system of the disease has a good biophysical significance and medical value.
Keywords:  myelin basic protein      liquid crystal monolayers      synergy factor      surface morphology  
Received:  11 May 2016      Revised:  29 June 2016      Accepted manuscript online: 
PACS:  05.70.-a (Thermodynamics)  
Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 21402114 and 11544009), the Natural Science Basic Research Plan in Shaanxi Province of China (Grant No. 2016JM2010), the Fundamental Research Funds for the Central Universities of China (Grant No. GK201604004), and the National University Science and Technology Innovation Project of China (Grant Nos. 201610718014 and cx16018).
Corresponding Authors:  Changchun Hao, Runguang Sun     E-mail:;

Cite this article: 

Lei Zhang(张蕾), Changchun Hao(郝长春), Ying Feng(冯盈), Feng Gao(高峰), Xiaolong Lu(逯晓龙), Junhua Li(李俊花), Runguang Sun(孙润广) Analysis of the induction of the myelin basic protein binding to the plasma membrane phospholipid monolayer 2016 Chin. Phys. B 25 090507

[1] Kowal J, Wu D L, Mikhalevich V, Palivan C G and Meier W 2015 Langmuir 31 5b00388
[2] López-Oyama A B, Flores-Vázquez A L, Burboa M G, Gutiérrez-Millán L E and Ruiz-García J 2009 J. Phys. Chem. B 113 9802
[3] Tu Z C 2013 Chin. Phys. B 22 028701
[4] Motschmann H and Lunkenheimer K 2002 J. Colloid Interf. Sci. 248 462
[5] Phadungbut P, Do D D, Nicholson D and Tangsathitkulchai C 2015 Chem. Eng. Sci. 126 257
[6] Ou-Yang Z C 2003 Chinese Journal of Liquid Crystals and Displays 18 79
[7] Wen-Jiang Y E, Liu X S, Song X L, Zheng G L and Sciences S O 2014 Chinese Journal of Liquid Crystals and Displays 29 686
[8] Ji Y, Luo Y F, Jia X R, Chen E Q, Huang Y, Ye C and Wang B B 2005 Angew. Chem. Int. Edit. 44 6025
[9] Cui N, Zhang J, Li H Y and Song D 2015 Chinese Journal of Liquid Crystals 30 57
[10] Caillé A, Pink D, Verteuil F D and Zuckermann M J 1980 Can. J. Phys. 58 581
[11] Vassilev V M, Djondjorov P A and Mladenov I M 2013 Chin. Phys. B 22 028701
[12] Bisoyi H K and Kumar S 2010 Chem. Soc. Rev. 39 264
[13] SamuliOllila O H, Lamberg A, Lehtivaara M, Koivuniemi A and Vattulainen I 2012 Biophys. J. 10 1236
[14] Lee J and Chang C H 2014 Soft Matter 10 1831
[15] Knoll W, Peters J, Kursula P, Gerelli Y and Ollivier J 2014 Soft Matter 10 519
[16] Facci P, Cavatorta P, Cristofolini L, Fontana M P, Fasano A and Riccio P 2000 Biophys. J. 78 1413
[17] Lanteri N, Rolandi R, Cavatorta P, Polverini E, Riccio P and Gliozzi A 2000 Colloid Surface A 175 3
[18] Nawaz S, Kippert A, Saab A S, Hauke B W, Lang T, Nave K A and Simons M 2009 J. Neurosci. 29 4794
[19] Lee D W, BanquyX, Kristiansen K, Kaufman Y, Boggs J M and Israelachvili J N 2014 PNAS 111 E768
[20] Lee D W, Min Y, Dhar P, Ramachandran A, Israelachvili J N and Zasadzinski J A 2011 PNAS 108 9425
[21] Kenrick A V, Vladimir V B and Harauz G 2015 Biochem. J. 472 17
[22] Boggs J M 2006 Cell. Mol. Life Sci. 63 1945
[23] Harauz G, Ladizhansky V and Boggs J M 2009 Biochem. 48 8094
[24] Pelosi P, Zhou J J, Ban L P and Calvello M 2006 Cell. Mol. Life Sci. 63 1658
[25] Lee D W, Banquy X, Kristiansen K, Min Y, Ramachandran A, Boggs J M and Israelachvili J N 2015 Langmuir 31 3159
[26] Czogalla A, Grzybek M, Jones W and Coskun Ü 2014 Biochim. Biophys. Acta 1841 1049
[27] Li X F, Budai J D, Liu F, et al. 2013 Science & Applications 2 e50
[28] Wang L, Lin X W, Hu W, et al. 2015 Science & Applications 4 e253
[29] Rispoli P, Carzino R, SvaldoL T, et al. 2007 Biophy. J. 93 1999
[30] Stapulionis R and Oliveira C L P 2008 J. Immunol. 180 3946
[31] Hu Y F, Doudevski I, Wood D, Moscarello M, Husted C, Genain C, Zasadzinski J A and Israelachvili J 2004 PNAS 101 13466
[32] Li Z J, Li Q, Cheng Z G, Li H B and Fang Y 2014 Chin. Phys. B 23 028102
[33] Xie Y Z, Liu X Q and Ou-Yang Z C 2003 China Science and Technology 8
[34] Li H B, Jin L and Qiu B 2008 Chin. Phys. Lett. 25 4042
[35] Zhang Z C, You Z and Chu D P 2014 Science & Applications 3 e213
[36] Katarzyna H W, Anna Z and Patrycja D L 2011 J. Colloid Interf. Sci. 360 681
[37] Kaganer V M, Möhwald H and Dutta P 1999 Rev. Mod. Phys. 71 779
[38] Kim K, Choi S Q, Zasadzinski J A and Squires T M 2011 Soft Matter 7 7782
[39] Stefaniu C, Brezesinski G and Mohwald H 2012 Soft Matter 8 7952
[40] ZuckermannM J and Heimburg T 2001 Biophys. J. 81 2458
[41] Gallier S, Gragson D, Rafael J F and David W E 2010 J. Agric. Food Chem. 58 12275
[42] Calvez P, Bussieres S, Demers E and Salesse C 2009 Biochimie 91 718
[43] Boisselier É, Calvez P, Demers É, Cantin L and Salesse C 2012 Langmuir 28 9680
[1] Surface structure modification of ReSe2 nanosheets via carbon ion irradiation
Mei Qiao(乔梅), Tie-Jun Wang(王铁军), Yong Liu(刘泳), Tao Liu(刘涛), Shan Liu(刘珊), and Shi-Cai Xu(许士才). Chin. Phys. B, 2023, 32(2): 026101.
[2] Influence of N+ implantation on structure, morphology, and corrosion behavior of Al in NaCl solution
Hadi Savaloni, Rezvan Karami, Helma Sadat Bahari, Fateme Abdi. Chin. Phys. B, 2020, 29(5): 058102.
[3] Low-temperature plasma enhanced atomic layer deposition of large area HfS2 nanocrystal thin films
Ailing Chang(常爱玲), Yichen Mao(毛亦琛), Zhiwei Huang(黄志伟), Haiyang Hong(洪海洋), Jianfang Xu(徐剑芳), Wei Huang(黄巍), Songyan Chen(陈松岩), Cheng Li(李成). Chin. Phys. B, 2020, 29(3): 038102.
[4] Behavior of lysozyme adsorbed onto biological liquid crystal lipid monolayer at the air/water interface
Xiaolong Lu(逯晓龙), Ruixin Shi(史瑞新), Changchun Hao(郝长春), Huan Chen(陈欢), Lei Zhang(张蕾), Junhua Li(李俊花), Guoqing Xu(徐国庆), Runguang Sun(孙润广). Chin. Phys. B, 2016, 25(9): 090506.
[5] Effects of annealing temperature on shape transformation and optical properties of germanium quantum dots
Alireza Samavati, Z. Othaman, S. K. Ghoshal, M. K. Mustafa. Chin. Phys. B, 2015, 24(2): 028103.
[6] Effect of additional silicon on titanium/4H-SiC contacts properties
Zhang Yong-Ping (张永平), Chen Zhi-Zhan (陈之战), Lu Wu-Yue (卢吴越), Tan Jia-Hui (谈嘉慧), Cheng Yue (程越), Shi Wang-Zhou (石旺舟). Chin. Phys. B, 2014, 23(5): 057303.
[7] Microstructure and its influence on CH4 adsorption behavior of deep coal
Feng Yan-Yan (冯艳艳), Jiang Cheng-Fa (江成发), Liu Dai-Jun (刘代俊), Chu Wei (储伟). Chin. Phys. B, 2014, 23(2): 028201.
[8] Germanium nanoislands grown by radio frequency magnetron sputtering:Annealing time dependent surface morphology and photoluminescence
Alireza Samavati, Z. Othaman, S. K. Ghoshal, R. J. Amjad. Chin. Phys. B, 2013, 22(9): 098102.
[9] Effect of InxGa1-xN “continuously graded” buffer layer on InGaN epilayer grown by metalorganic chemical vapor deposition
Qian Wei-Ning (钱卫宁), Su Shi-Chen (宿世臣), Chen Hong (陈弘), Ma Zi-Guang (马紫光), Zhu Ke-Bao (朱克宝), He Miao (何苗), Lu Ping-Yuan (卢平元), Wang Geng (王耿), Lu Tai-Ping (卢太平), Du Chun-Hua (杜春花), Wang Qiao (王巧), Wu Wen-Bo (吴汶波), Zhang Wei-Wei (张伟伟). Chin. Phys. B, 2013, 22(10): 106106.
[10] Thickness dependence of grain size and surface roughness for dc magnetron sputtered Au films
Zhang Xin(张鑫), Song Xiao-Hui(宋小会), and Zhang Dian-Lin(张殿琳). Chin. Phys. B, 2010, 19(8): 086802.
[11] Pulsed ion beam-assisted carburizing of titanium in methane discharge
M. Shafiq, M. Hassan, K. Shahzad, A. Qayyum, S. Ahmad, R. S. Rawat, and M. Zakaullah. Chin. Phys. B, 2010, 19(1): 012801.
[12] Dopant diffusion and surface morphology of vanadium implanted 4H-silicon carbide
Wang Chao(王超), Zhang Yi-Men(张义门), Zhang Yu-Ming(张玉明), Ma Ge-Lin(马格林), Guo Hui(郭辉), and Xu Da-Qing(徐大庆). Chin. Phys. B, 2007, 16(8): 2455-2461.
No Suggested Reading articles found!