Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition

doi:10.1088/1674-1056/24/12/128201

Abstract In this study, a lipid membrane was fabricated by fusing cardiolipin-phosphatidylcholine (CL_PC, 1:4) vesicles onto a hydrophobic surface of 1-dodecanethiol (DT) preadsorbed on a nanostructured gold film. By changing the concentration of the DT adsorption solution, we constructed a series of CL_PC-DT bilayers with different hydrophobicity to study the effects of lipid membrane characteristics on the adsorption conformation of cytochrome c (Cyt c). Electrochemical analysis showed that the formal potential is 0.24 V for Cyt c-CL_PC-DT(10), 0.2 V for Cyt c-CL_PC-DT(20), and 0.16 V for Cyt c-CL_PC-DT(40)–a gradual positive shift with the decreasing DT concentration–relative to the potential of native cyt c (0.02 V). Potential-induced surface-enhanced infrared adsorption difference spectroscopy revealed that the gradual positive shift of the formal potential of CL-bound cyt c is determined by the environment with the gradually lowered dielectric constant for the heme cofactor in CL-bound cyt c (Fe³⁺).

Keywords: cytochrome c cardiolipin surface-enhanced infrared adsorption spectroscopy protein-solvent interaction

Received: 30 September 2015
Revised: 20 October 2015
Accepted manuscript online:

PACS:	82.20.Yn	(Solvent effects on reactivity)
	82.39.Jn	(Charge (electron, proton) transfer in biological systems)
	82.45.Mp	(Thin layers, films, monolayers, membranes)
	82.45.Tv	(Bioelectrochemistry)

Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 91227114, 21322510, and 21105097), the China Postdoctoral Science Foundation (Grant No. 2013M530998), the Natural Science Foundation of Jilin Province, China (Grant No. 201215092), and the President Funds of the Chinese Academy of Sciences.

Corresponding Authors: Jiang Xiu-E
E-mail: jiangxiue@ciac.ac.cn

Cite this article:

Liu Li (刘丽), Wu Lie (武烈), Zeng Li (曾丽), Jiang Xiu-E (姜秀娥) Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition 2015 Chin. Phys. B 24 128201

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Label-free surface-enhanced infrared spectro-electro-chemical analysis of the Redox potential shift of cytochrome c complexed with a cardiolipin-containing lipid membrane of varied composition

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