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In situ calibrating optical tweezers with sinusoidal-wave drag force method |
| Li Di (李迪)a, Zhou Jin-Hua (周金华)a, Hu Xin-Yao (呼新尧)a, Zhong Min-Cheng (钟敏成)a, Gong Lei (龚雷)a, Wang Zi-Qiang (王自强)a, Wang Hao-Wei (王浩威)a, Li Yin-Mei (李银妹)a b |
a Department of Optics and Optical Engineering, University of Science and Technology of China, Hefei 230026, China;
b Hefei National Laboratory for Physical Sciences at the Microscale, Hefei 230026, China |
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Abstract We introduce a corrected sinusoidal-wave drag force method (SDFM) into optical tweezers to calibrate the trapping stiffness of the optical trap and conversion factor (CF) of photodetectors. First, the theoretical analysis and experimental result demonstrate that the correction of SDFM is necessary, especially the error of no correction is up to 11.25% for a bead of 5 μm in diameter. Second, the simulation results demonstrate that the SDFM has a better performance in the calibration of optical tweezers than the triangular-wave drag force method (TDFM) and power spectrum density method (PSDM) at the same signal-to-noise ratio or trapping stiffness. Third, in experiments, the experimental standard deviations of calibration of trapping stiffness and CF with the SDFM are about less than 50% of TDFM and PSDM especially at low laser power. Finally, the experiments of stretching DNA verify that the in situ calibration with the SDFM improves the measurement stability and accuracy.
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Received: 30 April 2015
Revised: 15 June 2015
Accepted manuscript online:
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PACS:
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87.80.Cc
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(Optical trapping)
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42.50.Wk
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(Mechanical effects of light on material media, microstructures and particles)
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87.14.gk
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(DNA)
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| Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 11302220, 11374292, and 31100555) and the National Basic Research Program of China (Grant No. 2011CB910402). |
Corresponding Authors:
Li Yin-Mei
E-mail: liyinmei@ustc.edu.cn
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Cite this article:
Li Di (李迪), Zhou Jin-Hua (周金华), Hu Xin-Yao (呼新尧), Zhong Min-Cheng (钟敏成), Gong Lei (龚雷), Wang Zi-Qiang (王自强), Wang Hao-Wei (王浩威), Li Yin-Mei (李银妹) In situ calibrating optical tweezers with sinusoidal-wave drag force method 2015 Chin. Phys. B 24 118703
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