Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin
Yang Shi-Xin (阳世新)a, Li Fang-Hua (李方华)b, Liu Yu-Dong (刘玉东)c, Wang Huai-Bin (王怀斌)b, Gu Yuan-Xin (古元新)b, Fan Hai-Fu (范海福)b
a College of Life Science, Sichuan University, Chengdu 610064, China; b Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, China; c Lineberger Comprehensive Cancer Centre, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA
Abstract Direct-method phase extension has been applied to two-dimensional electrondiffraction data of the protein streptavidin. Structure-factor amplitudesfrom electron diffraction were combined with phases from the correspondingelectron micrographs. Maximum-entropy discrimination and cluster analysiswere used to derive a solution from a large number of random trials. The phase extension from 0.3 to 0.25nm led to substantial improvement of thereconstructed projection image quality.
Received: 06 July 2004
Revised: 13 August 2004
Accepted manuscript online:
Fund: Natural Science Foundation of China (Grant Nos 19574073 and19634020)
Cite this article:
Yang Shi-Xin (阳世新), Li Fang-Hua (李方华), Liu Yu-Dong (刘玉东), Wang Huai-Bin (王怀斌), Gu Yuan-Xin (古元新), Fan Hai-Fu (范海福) Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin 2005 Chinese Physics 14 1
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