Please wait a minute...
Chinese Physics, 2005, Vol. 14(1): 1-5    DOI: 10.1088/1009-1963/14/1/001
GENERAL   Next  

Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin

Yang Shi-Xin (阳世新)a, Li Fang-Hua (李方华)b, Liu Yu-Dong (刘玉东)c, Wang Huai-Bin (王怀斌)b, Gu Yuan-Xin (古元新)b, Fan Hai-Fu (范海福)b
a College of Life Science, Sichuan University, Chengdu 610064, China; b Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, China; c Lineberger Comprehensive Cancer Centre, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA
Abstract  Direct-method phase extension has been applied to two-dimensional electrondiffraction data of the protein streptavidin. Structure-factor amplitudesfrom electron diffraction were combined with phases from the correspondingelectron micrographs. Maximum-entropy discrimination and cluster analysiswere used to derive a solution from a large number of random trials. The phase extension from 0.3 to 0.25nm led to substantial improvement of thereconstructed projection image quality.
Keywords:  electron crystallography      direct method      protein structure      streptavidin  
Received:  06 July 2004      Revised:  13 August 2004      Accepted manuscript online: 
PACS:  0290  
  6165  
Fund: Natural Science Foundation of China (Grant Nos 19574073 and19634020)

Cite this article: 

Yang Shi-Xin (阳世新), Li Fang-Hua (李方华), Liu Yu-Dong (刘玉东), Wang Huai-Bin (王怀斌), Gu Yuan-Xin (古元新), Fan Hai-Fu (范海福) Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin 2005 Chinese Physics 14 1

[1] Enhancement of MAD/MIR phasing at low resolution and a new procedure for automatic phase extension
Pu Han(韩普), Yuan-Xin Gu(古元新), Wei Ding(丁玮), Hai-Fu Fan(范海福). Chin. Phys. B, 2019, 28(7): 076108.
[2] On the parameters for electrocaloric effect predicted by indirect method
Hong-Bo Liu(刘宏波). Chin. Phys. B, 2017, 26(11): 117701.
[3] Structural modeling of proteins by integrating small-angle x-ray scattering data
Zhang Yong-Hui (张泳辉), Peng Jun-Hui (彭俊辉), Zhang Zhi-Yong (张志勇). Chin. Phys. B, 2015, 24(12): 126101.
[4] Proteins:From sequence to structure
Zheng Wei-Mou (郑伟谋). Chin. Phys. B, 2014, 23(7): 078705.
[5] Finite symmetry transformation group and localized structures of (2+1)-dimensional coupled Burgers equation
Lei Ya (雷娅), Yang Duo (杨铎). Chin. Phys. B, 2013, 22(4): 040202.
[6] Approximate homotopy similarity reduction for the generalized Kawahara equation via Lie symmetry method and direct method
Liu Xi-Zhong(刘希忠). Chin. Phys. B, 2010, 19(8): 080202.
[7] New expression of bimodal phase distributions in direct-method phasing of protein single-wavelength anomalous diffraction data
Zhang Tao (张涛), Gu Yuan-Xin (古元新), Zheng Chao-De (郑朝德), Fan Hai-Fu (范海福). Chin. Phys. B, 2010, 19(8): 086102.
[8] A revised version of the program VEC (visual computing in electron crystallography)
Li Xue-Ming(李雪明), Li Fang-Hua(李方华), and Fan Hai-Fu(范海福). Chin. Phys. B, 2009, 18(6): 2459-2463.
[9] Direct method of finding first integral of two-dimensional autonomous systems in polar coordinates
Lou Zhi-Mei (楼智美), Wang Wen-Long (汪文珑). Chin. Phys. B, 2006, 15(5): 895-898.
[10] A certain critical two-soliton solution of the nonlinear Schr?dinger equation
Yan Jia-Ren (颜家壬), Pan Liu-Xian (潘留仙), Lu Jing (卢竞). Chin. Phys. B, 2004, 13(4): 441-444.
[11] Phasing of lysozyme using one-wavelength anomalous scattering of sulphur atoms through the combination of direct methods and density modification
Jiang Fan (江凡), Gu Yuan-Xin (古元新), Zheng Chao-De (郑朝德), Fan Hai-Fu (范海福). Chin. Phys. B, 2003, 12(8): 890-894.
[12] Solving a superstructure from two-wavelength x-ray powder diffraction data - a simulation
Chen Jian-Rong (陈建荣), Gu Yuan-Xin (古元新), Fan Hai-Fu (范海福). Chin. Phys. B, 2003, 12(3): 310-314.
[13] Ab-initio determination of the incommensurate modulated structure of Bi-2212 from x-ray powder diffraction data -a simulation
Chen Jian-Rong (陈建荣), Gu Yuan-Xin (古元新), Zheng Chao-De (郑朝德), Fan Hai-Fu (范海福). Chin. Phys. B, 2003, 12(11): 1261-1265.
[14] Study of the influence of higher-order Laue zones on the exit wavefunction
Liu Qiu-Xiang (刘秋香), Yang Qi-Bin (杨奇斌), Wang Yuan-Ming (王元明). Chin. Phys. B, 2002, 11(6): 587-591.
[15] SIMILARITY REDUCTIONS OF THE (2+1)-DIMENSIONAL BURGERS SYSTEM
Liu Dang-bo (刘当波), Chu Kai-qin (储开芹). Chin. Phys. B, 2001, 10(8): 683-688.
No Suggested Reading articles found!