|
Special Issue:
SPECIAL TOPIC — Celebrating the 100th Anniversary of Physics Discipline of Northwest University
|
| SPECIAL TOPIC—Celebrating the 100th Anniversary of Physics Discipline of Northwest University |
Prev
Next
|
|
|
Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel |
| Jie Zhu(朱洁), Jing Xue(薛菁), Wei Zhao(赵伟), Chen Zhang(张琛), Xiaoqiang Feng(冯晓强), and Kaige Wang(王凯歌)† |
| State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Key Laboratory of Optoelectronic Technology of Shaanxi Province, National Center for International Research of Photoelectric Technology & Nano-functional Materials and Application, Institute of Photonics and Photon-Technology, Northwest University, Xi'an 710069, China |
|
|
|
|
Abstract Interactions between deoxyribonucleic acid (DNA) and metal ions are vital for maintaining life functions, however, there are still unsolved questions about its mechanisms. It is of great practical significance to study these issues for medical chip design, drug development, health care, etc. In this investigation, the conductivity properties of λ -DNA solutions with mono-/divalent metal ions (Na+, K+, Mg2+, and Ca2+) are experimentally studied as they are electrically driven through a 5 μ m microfluidic channel. Experimental data indicate that the conductivities of λ -DNA solutions with metal ions (M+/M2+) basically tend to reduce firstly and then increase as the voltage increases, of which the turning points varied with the metal ions. When the voltage surpasses turning points, the conductivity of λ -DNA-M+ solutions increases with the concentration of metal ions, while that of λ -DNA-M2+ solutions decrease. Moreover, the conductivity of λ -DNA-M2+ solutions is always smaller than that of λ -DNA-M+ solutions, and with high-concentration M2+, it is even smaller than that of the λ -DNA solution. The main reasons for the above findings could be attributed to the polarization of electrodes and different mechanisms of interactions between metal ions and λ -DNA molecules. This investigation is helpful for the precise manipulation of single DNA molecules in micro-/nanofluidic space and the design of new biomedical micro-/nanofluidic sensors.
|
Received: 30 June 2023
Revised: 28 August 2023
Accepted manuscript online: 19 September 2023
|
|
PACS:
|
87.14.gk
|
(DNA)
|
| |
87.19.rh
|
(Fluid transport and rheology)
|
| |
47.27.nd
|
(Channel flow)
|
| |
87.15.H-
|
(Dynamics of biomolecules)
|
|
| Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 62275216 and 61775181), the Innovation Capability Support Program of Shaanxi Province of China (Grant Nos. S2018-ZC-TD-0061 and TZ0393), and the National Key Scientific Instrument and Equipment Development Projects of China (Grant No. 51927804). |
Corresponding Authors:
Kaige Wang
E-mail: wangkg@nwu.edu.cn
|
Cite this article:
Jie Zhu(朱洁), Jing Xue(薛菁), Wei Zhao(赵伟), Chen Zhang(张琛), Xiaoqiang Feng(冯晓强), and Kaige Wang(王凯歌) Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel 2023 Chin. Phys. B 32 118704
|
[1] Alberts B, Johnson A, Lewis J, Rafi M, Roberts K and Walter P 2014 Mol. Biol. Cell 6th edn. (London:Garland Science)
[2] Chen T, Ren L, Liu X, Zhou M, Li L, Xu J and Zhu X 2018 Int. J. Mol. Sci. 19 1671
[3] Zhao L Y, Song J, Liu Y, Song C X and Yi C 2020 Protein Cell 11 792
[4] Wang J Y, Pausch P and Doudna J A 2022 Nat. Rev. Microbiol. 20 641
[5] Chellan P and Sadler P J 2015 Philos. Trans. A Math. Phys. Eng. Sci. 373 1364
[6] Maret W 2016 Int. J. Mol. Sci. 17 66
[7] Akanuma G 2021 Biosci. Biotechnol. Biochem. 85 1582
[8] Pak K, Ordway S, Sadowski B, Canevari M and Torres D 2021 Clin. Liver Dis. 17 61
[9] Anastassopoulou J, Anifantakis B, Anifantakis Z A, Dovas A and Theophanides T 2000 J. Inorg. Biochem. 79 327
[10] Theophanides T and Anastassopoulou J 2002 Crit. Rev. Oncol. Hemetol. 42 57
[11] Waldbaur A, Rapp H, Länge K and Rapp B E 2011 Anal. Methods 3 2681
[12] Zhou W, Dou M, Timilsina S S, Xu F and Li X 2021 Lab Chip 21 2658
[13] Radhakrishnan S, Mathew M and Rout C S 2022 Adv. Mater. 3 1874
[14] Stein D, van den Heuvel M G L and Dekker C 2016 Nanofluidics 2nd edn. (Cambridge:The Royal Society of Chemistry)
[15] Bockrath M, Markovic N, Shepard A and Tinkham M 2002 Nano Lett. 3 187
[16] Liu S, Clever G H, Takezawa Y, Kaneko M, Tanaka K, Guo X and Shionoya M 2011 Angew. Chem. 50 8886
[17] Ma H, Wang K, Gao Z, Wang H, Wang S, Zhang C, Wang G and Bai J 2014 AIP Adv. 4 107139
[18] Duan Y, Ma H, Gao Z, Wang K, Zhao W, Sun D, Wang G, Li J, Bai J and Gu C 2017 Chin. Phys. B 26 068203
[19] Duan Y, Zhao W, Xue J, Sun D, Wang K, Wang G, Li J, Bai J and Gu C 2017 J. Phys. D:Appl. Phys. 50 125401
[20] Zhu J, Xue J, Sun D, Zhao W, Zhang C, Feng X and Wang K 2022 Langmuir 38 1716
[21] Xue J, Zhao W, Nie T, Zhang C, Ma S, Wang G, Liu S, Li J, Gu C, Bai J and Wang K 2018 Langmuir 34 15203
[22] Egli M 2002 Chem. Biol. 9 277
[23] Burda J V, Sponer J, Leszczynski J and Hobza P 1997 J. Phys. Chem. B 101 9670
[24] Anastassopoulou J 2003 J. Mol. Struct. 651-653 19
[25] McFail-Isom L, Shui X and Williams L D 1998 Biochemistry 37 17105
[26] Hud N V and Polak M 2001 Curr. Opin. Struct. Biol. 11 293
[27] Turel I and Kljun J 2011 Curr. Top. Med. Chem. 11 2661
[28] Turel I and Kljun J 2011 Curr. Top. Med. Chem. 11 2661
[29] Anastassopoulou J 2003 J. Mol. Struct. 651-653 19
[30] Manning G S 1978 Quart. Rev. Biophys. 11 179
[31] Bloomfield V A 1998 Biopolymers 44 269
[32] Besteman K, Van Eijk K and Lemay S G 2007 Nat. Phys. 3 641
[33] Lyklema J, University W and Wageningen 1995 Fundamentals of Interface and Colloid Science 1st edn. (New York:Academic Press)
[34] Kirby B 2010 Micro- and Nanoscale Fluid Mechanics:Transport in Microfluidic Devices 1st edn. (New York:Cambridge University Press)
[35] Grahame D C 1947 Chem. Rev. 41 441
[36] Allagui A, Benaoum H and Olendski O 2021 Physica A 582 126252 |
| No Suggested Reading articles found! |
|
|
Viewed |
|
|
|
Full text
|
|
|
|
|
Abstract
|
|
|
|
|
Cited |
|
|
|
|
Altmetric
|
|
blogs
Facebook pages
Wikipedia page
Google+ users
|
Online attention
Altmetric calculates a score based on the online attention an article receives. Each coloured thread in the circle represents a different type of online attention. The number in the centre is the Altmetric score. Social media and mainstream news media are the main sources that calculate the score. Reference managers such as Mendeley are also tracked but do not contribute to the score. Older articles often score higher because they have had more time to get noticed. To account for this, Altmetric has included the context data for other articles of a similar age.
View more on Altmetrics
|
|
|
