Dielectric properties of nucleated erythrocytes as simulated by the double spherical-shell model

doi:10.1088/1674-1056/abbbf0

Abstract The dielectric properties of nucleated erythrocytes from bullfrogs were measured in a frequency range of 10 kHz-110 MHz. The complex permittivity (ε^*), complex conductivity ($\kappa^*$), and complex resistivity (ρ^*) were analyzed and compared in the 10.63% to 37.58% haematocrit (Hct) range. The relaxation behavior, the passive electrical properties, and the cellular structure parameters, including the cell membrane, the cytoplasm, the nuclear membrane, and the nucleoplasm of the nucleated erythrocyte suspensions were investigated. The method used is based on the binomial Cole-Cole equation and the double spherical-shell physical models. Upon the elimination of the electrode polarization effect, two definite relaxations related to the interfacial polarization are observed on low-and high-frequency dispersions. The permittivity values and the characteristic frequency values differ by one order of magnitude: the low-frequency relaxation increments [∆ ε ₁= (5.63 1.43)× 10³] and the characteristic frequency [f_c1= (297.06 14.48) kHz] derived from the cell membrane, the high-frequency relaxation increments [∆ ε ₂ =(5.21 1.20)× 10²] and the characteristic frequency [f_c2=(3.73 0.06) MHz] derived from the dielectric response to the external electric field of the nuclear membrane, respectively. Moreover, the other core dielectric parameters, such as the relative permittivity of the cell membrane [ε_m= (7.57 0.38)] and the nuclear envelope [ε_ne= (23.59 4.39)], the conductivity of the cytoplasm (hemoglobin, $\kappa_\rm Hb= (0.50 \pm 0.13)$ S/m] and the nuclear endoplasm [$\kappa_\rm np= (2.56 \pm 0.75)$ S/m], and the capacitance of the bilayer membranes [C_m: (0.84 0.04) μ F/cm²], and C_ne: (0.52 0.10) μ F/cm²] were also accurately and reliably measured. This work presents a feasible method to evaluate the dielectric parameters and the cellular structure of the erythrocytes of bullfrogs. Moreover, it paves the way for new studies on the haematology of frogs and the detection of nucleated cells via dielectric impedance spectroscopy.

Keywords: dielectric impedance spectroscopy bullfrog erythrocytes the double spherical-shell model passive electrical property

Received: 23 July 2020
Revised: 16 September 2020
Accepted manuscript online: 28 September 2020

PACS:	87.19.rf	(Dielectric properties)
	84.37.+q	(Measurements in electric variables (including voltage, current, resistance, capacitance, inductance, impedance, and admittance, etc.))

Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 51277099 and 52007087), the Natural Science Foundation of Zhejiang Province, China (Grant Nos. LY20C110001 and LSY19A010002), the Natural Science Foundation of Ningbo City, China (Grant Nos. 2019A610349 and 202003N4116), the Fund from the Educational Commission of Zhejiang Province, China (Grant No. Y202044047), and the Fundamental Research Funds for the Provincial Universities of Zhejiang Province, China.

Corresponding Authors: ^†Corresponding author. E-mail: maqing@nbu.edu.cn

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Jia Xu(徐佳), Weizhen Xie(谢伟珍), Yiyong Chen(陈一勇), Lihong Wang(王立洪), and Qing Ma(马青) Dielectric properties of nucleated erythrocytes as simulated by the double spherical-shell model 2020 Chin. Phys. B 29 128703

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Dielectric properties of nucleated erythrocytes as simulated by the double spherical-shell model

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