| ELECTROMAGNETISM, OPTICS, ACOUSTICS, HEAT TRANSFER, CLASSICAL MECHANICS, AND FLUID DYNAMICS |
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Highly sensitive, ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe |
| Yufang Chen(陈彧芳)1,†, Wenbo Zhao(赵文博)2, Jinxing Xiong(熊金鑫)2, Jiashu Zhang(张家树)1, Kai Zhang(张开)2, Jiale Xie(解嘉乐)2, Menghan Liu(刘梦涵)1, and Hongdan Wan(万洪丹)2,‡ |
1 School of Network and Communication, Nanjing Vocational College of Information Technology, Nanjing 210023, China;
2 Advanced Photonic Technology Laboratory, Nanjing University of Posts and Telecommunications, Nanjing 210023, China |
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Abstract A highly sensitive, ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe (ROFP) is proposed and demonstrated. Our approach achieves a mass limit of detection of $\sim10$ aM based on the ROFP. A particular single-nucleotide mismatch sequence is also identified. The sensitivity of the proposed DNA biosensor is 1.51 nm/lgaM, about three-fold higher than using single-strand DNA probes (0.47 nm/lgaM) and with high specificity. The proposed ROFP has high compactness (with a length of $\sim3 $ mm) which is convenient for sample detection with small volume and complex gradients in small spaces with high sensitivity.
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Received: 01 August 2025
Revised: 03 September 2025
Accepted manuscript online: 15 September 2025
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| Fund: This work was supported by the Natural Science Foundation of the Jiangsu Higher Education Institutions of China (Grant Nos. 25KJB416001 and 22KJB510034), the National Natural Science Foundation of China (Grant Nos. 12174199 and 11704199), the Scientific Research Foundation for Highlevel Talents in Nanjing Vocational College of Information Technology (Grant No. YB202410), the China Postdoctoral Science Foundation (Grant No. 2021M701765), General Program of Natural Science Foundation of Jiangsu Province (Grant No. BK20221330), Jiangsu University ‘Blue Project’ Funding, Postgraduate Research & Practice Innovation Programs of Jiangsu Province, and Jiangsu Province Higher Education Teaching Reform Research Project (Grant No. 2025JGYB487). |
Corresponding Authors:
Yufang Chen, Hongdan Wan
E-mail: chenyf@njcit.cn;hdwan@njupt.edu.cn
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Cite this article:
Yufang Chen(陈彧芳), Wenbo Zhao(赵文博), Jinxing Xiong(熊金鑫), Jiashu Zhang(张家树), Kai Zhang(张开), Jiale Xie(解嘉乐), Menghan Liu(刘梦涵), and Hongdan Wan(万洪丹) Highly sensitive, ultra-low detection limit 3-D DNA nanostructure biosensor based on functionalized reflective optical fiber probe 2026 Chin. Phys. B 35 054207
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[1] Gilboa T, Garden P M and Cohen L 2020 Anal. Chim. Acta 61 1115
[2] Wang C H, Zhang Y, Huang T W and Yang Z C 2024 IEEE Trans. Netw. Sci. Eng. 11 6550
[3] Zhang H Y and Miller B L 2019 Biosens. Bioelectron. 141 111476
[4] Ribeiro B V, Cordeiro T A R, Freitas G R O, Ferreira L F and Franco D L 2020 Talanta Open 2 100007
[5] Kong J W, Dou S X, Li W, Li H and Wang P Y 2023 Chin. Phys. Lett. 40 078701
[6] Zhao S Q, Jia Y X, Wang A P and Yang L 2023 Biosens. Bioelectron. 239 115613
[7] Fan Z Q, Yao B, Ding Y D, Zhao J and Xie M H 2021 Biosens. Bioelectron. 178 113015
[8] Li Z, Yi Y, Luo X, et al. 2020 J. med. virol. 92 1518
[9] Shaimerdenova M, Ayupova T, Ashikbayeva Z, Bekmurzayeva A, Blanc W and Tosi D 2023 J. Lightwave Technol. 41 4114
[10] Saha U, Madaboosi N, Sai V V R and Bandyopadhyay S 2024 IEEE Sens. Lett. 8 5000104
[11] Wu T, Ding L Y, Zhang YMand FangWu 2022 IEEE Sens. J. 22 12485
[12] Lang X Z, Singh R, Zhang B Y and Kumar S 2024 IEEE Sens. J. 24 1564
[13] Delgado-Pinar M, Shi Q, Poveda-Wong L, et al. 2017 IEEE Sens. J. 17 5503
[14] Li X S, Singh R, Zhang B Y, et al. 2024 IEEE Sens. J. 24 4480
[15] Wan H D, Chen X Y, Zhang S T, Xiong J X, Jiang W F, Liu H Z, Wan C, Wang J L, Xiao Y, Wu Z J and Chao J 2024 Opt. Express 32 31514
[16] Cao H Y, Wei D D, Hu Y Y, Liu B, Fu Y and Liu J 2024 IEEE Sens. J. 24 31735
[17] Gong P Q, Li X G, Zhao Q M, Zhou X, Zhang Y N and Zhao Y 2024 Opt. Laser Technol. 168 109978
[18] Wen H Y, Huang C W, Li Y L, et al. 2020 Sensors 20 1509
[19] Li Y P, Fang F, Yang L Y, Tan S J, Yan Z J and Sun Q Z 2020 Opt. Express 28 970
[20] Li L L, Yu S, Jing C L, Chen L H and Zhou A 2024 IEEE Sens. J. 24 17387
[21] Ran Y, Xu Z Y, Chen M F, Guan B O, et al. 2022 Advanced Science 9 2200456
[22] Li H T, Wang X, Wu H, et al. 2024 Biosens. Bioelectron. 264 116672
[23] Huang T Q, Lu L, Wang H Z, et al. 2022 ACS Sens. 7 1926
[24] Yuan H, Yang Y, et al. 2023 Anal. Chem. 95 5142
[25] Wang L and Chong L 2025 RSC adv. 15 1831
[26] Feng D Z, Su J, Xu Y, He G F, Wan C G, Wang X, Pan T R, Ding X T and Mi X Q 2021 Microsyst. Nanoeng. 7 33
[27] Mitchell N, Schlapak R, Kastner M, Armitage D, et al. 2009 Angew. Chem. Int. Ed. 48 525
[28] Su J S, Liu T S, Wang M, Xu W S, Liu J L, Lan J N, Chen Y J, Xu H, Guo D H and Lai Z M 2025 Anal. Chim. Acta 1334 343419
[29] Ding M, Wang P and Brambilla G 2012 Opt. Express 20 5402
[30] Yang F, Li Q, Wang L H, Zhang G J, Fan C H, et al. 2018 ACS Sens. 3 903
[31] Lin M H, Song P, Zhou G B, Fan C H, et al. 2016 Nat. Protoc. 11 1244
[32] ChenWJ, Ö zdemir Ş K, Zhao GM,Wiersig J and Yang L 2017 Nature 548 192
[33] Yang Q C, Xu Z Y, Long J Q, Wang H P, Ran Y and Guan B O 2025 Nat. Commun. 16 6250
[34] Wang Y J, Zhang H, Duan S X, Lin W, Liu B and Wu J X 2021 IEEE Sens. J. 21 9148
[35] Suhailin F H, Alwahib A A and Chee H Y 2022 IEEE Sens. J. 22 20516
[36] Yin Z Y, Zhang Z B and Jing X L 2025 Anal. Chim. Acta 1333 343385
[37] Zhou X, Hui Y P and Chen Z X 2024 Opt. Laser Technol. 177 111187
[38] Li X, Zhao Q and Zhang Y 2024 IEEE Trans. Instrum. Meas. 73 7003708 |
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