Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

doi:10.1088/1674-1056/25/7/078201

Abstract

The electrodynamic characteristics of single DNA molecules moving within micro-/nano-fluidic channels are important in the design of biomedical chips and bimolecular sensors. In this study, the dynamic properties of λ -DNA molecules transferring along the microchannels driven by the external electrickinetic force were systemically investigated with the single molecule fluorescence imaging technique. The experimental results indicated that the velocity of DNA molecules was strictly dependent on the value of the applied electric field and the diameter of the channel. The larger the external electric field, the larger the velocity, and the more significant deformation of DNA molecules. More meaningfully, it was found that the moving directions of DNA molecules had two completely different directions: (i) along the direction of the external electric field, when the electric field intensity was smaller than a certain threshold value; (ii) opposite to the direction of the external electric field, when the electric field intensity was greater than the threshold electric field intensity. The reversal movement of DNA molecules was mainly determined by the competition between the electrophoresis force and the influence of electro-osmosis flow. These new findings will theoretically guide the practical application of fluidic channel sensors and lab-on-chips for precisely manipulating single DNA molecules.

Keywords: reversal movement electrophoresis electroosmosis electric field threshold value

Received: 02 February 2016
Revised: 22 March 2016
Accepted manuscript online:

PACS:	82.37.Rs	(Single molecule manipulation of proteins and other biological molecules)
	87.15.H-	(Dynamics of biomolecules)
	87.15.Tt	(Electrophoresis)
	87.19.rh	(Fluid transport and rheology)

Fund:

Project supported by the National Natural Science Foundation of China (Grant No. 61378083), the International Cooperation Foundation of the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant No. 2011DFA12220), the Major Research Plan of National Natural Science Foundation of China (Grant No. 91123030), and the Natural Science Foundation of Shaanxi Province of China (Grant Nos. 2010JS110 and 2013SZS03-Z01).

Corresponding Authors: Kaige Wang
E-mail: wangkg@nwu.edu.cn

Cite this article:

Fengyun Yang(杨凤云), Kaige Wang(王凯歌), Dan Sun(孙聃), Wei Zhao(赵伟), Hai-qing Wang(王海青), Xin He(何鑫), Gui-ren Wang(王归仁), Jin-tao Bai(白晋涛) Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique 2016 Chin. Phys. B 25 078201

[1]	Ha T and Tinnefeld P 2012 Ann. Rev. Phys. Chem. 63 595
[2]	Mai D J, Brockman C and Schroeder C M 2012 Soft Matter 8 10560
[3]	Yu J, Xiao J, Ren X J, Lao K Q and Xie X S 2006 Science 311 1600
[4]	Cai L, Friedman N and Xie X S 2006 Nature 440 358
[5]	Mivelle M, Zanten T, Manzo C and Garcia-Parajo M F 2014 Microsc. Res. Tech. 77 537
[6]	Renner C B and Doyle P S 2015 Soft Matter 11 3105
[7]	Whitesides G M 2006 Nature 442 368
[8]	Mairhofer J, Roppert K and Ertl P 2009 Sensors 9 4804
[9]	Liu C, Qu Y Y, Luo Y and Fang N 2011 Electrophoresis 32 3308
[10]	Gupta C, Liao W C, Gallego-Perez D, Castro C and Lee L 2014 Biomicrofluidics 8 024114
[11]	Tegenfeldt J O, Prinz C, Cao H, Huang R L, Austin R H, Chou S Y, Cox E C and Sturm J C 2004 Anal. Bioanal. Chem. 378 1678
[12]	Min S K, Kim W Y, Cho Y and Kim K S 2011 Nat. Nanotechnol. 6 162
[13]	Mauk M G, Liu C, Sadik M and Bau H H 2015 Mobile Health Technologies: Methods and Protocols 1256 15
[14]	Firnkes M, Pedone D, Knezevic J, Doblinger M and Rant U 2010 Nano Lett. 10 2162
[15]	Tang J and Doyle P S 2007 Appl. Phys. Lett. 90 224103
[16]	Yeh J W, Taloni A, Chen Y L and Chou C F 2012 Nano Lett. 12 1597
[17]	Balducci A, Mao P, Han J and Doyle P S 2006 Macromolecules 39 6273
[18]	Yeo L Y, Chang H C, Chan P P and Friend J R 2011 Small 7 12
[19]	Santhiago M, Nery E W, Santos G P and Kubota L T 2014 Bioanalysis 6 89
[20]	Tang J 2010 Single Molecule DNA Dynamics in Micro-and Nano-fluidic Devices. Massachusetts Institute of Technology p.140
[21]	Chang H C and Yeo L Y 2010 Electrokinetically Driven Microfluidics and Nanofluidics (Cambridge : Cambridge University Press) p. 39
[22]	Zhang B, Wood M and Lee H 2009 Anal. Chem. 81 5541
[23]	Meller A and Branton D 2002 Electrophoresis 23 2583
[24]	Schoch R B, Han J and Renaud P 2008 Rev. Mod. Phys. 80 839
[25]	Liu Q J, Wu H W, Wu L Z, Xie X, Kong J L, Ye X F and Liu L P 2012 PLoS One 7 e46014
[26]	Bhattacharyya S and Gopmandal P P 2013 Soft Matter 9 1871
[27]	Tatsumi K, Nishitani K, Fukuda K, Katsumoto Y and Nakabe K 2010 Meas. Sci. Technol. 21 105402
[28]	Bown M, MacInnes J, Allen R and Zimmerman W 2006 Meas. Sci. Technol. 17 2175
[29]	Samouda F, Colin S, Barrot C, Baldas L and Brandner J J 2015 Microsyst. Technol. 21 527
[30]	Gerashchenko S and Steinberg V 2006 Phys. Rev. Lett. 96 038304
[31]	Teixeira R E, Babcock H P, Shaqfeh E S and Chu S 2005 Macromolecules 38 581
[32]	Hanasaki I, Yukimoto N, Uehara S, Shintaku H and Kawano S 2015 J. Phys. D: Appl. Phys. 48 135402
[33]	Manneschi C, Fanzio P, Ala-Nissila T, Angeli E, Repetto L, Firpo G and Valbusa U 2014 Biomicrofluidics 8 064121
[34]	Glasgow I, Batton J and Aubry N 2004 Lab on a Chip 4 558
[35]	Shaqfeh E S 2005 J. Non-Newtonian Fluid Mech. 130 1
[36]	Laib S, Robertson R M and Smith D E 2006 Macromolecules 39 4115
[37]	Lim Y, Kouzani A and Duan W 2010 Microsystem Technologies 16 1995
[38]	van Kan J A, Zhang C, Malar P P and van der Maarel J R 2012 Biomicrofluidics 6 036502
[39]	Xu W L and Muller S J 2012 Lab on a Chip 12 647
[40]	Perkins T T, Smith D and Chu S 1994 Science 264 822
[41]	Perkins T T, Smith D E and Chu S 1997 Science 276 2016
[42]	Smith D E, Babcock H P and Chu S 1999 Science 283 1724
[43]	Duan C H, Wang W and Xie Q 2013 Biomicrofluidics 7 026501
[44]	Kang G, No K and Kim G 2006 Int. J. Mod. Phys. B 20 4493
[45]	Becker H and Gärtner C 2008 Anal. Bioanal. Chem. 390 89
[46]	Yang Y Y, Wang K G, Qiao Q W, Li D, Wang S, Ren Z Y and Bai J 2012 12th IEEE Conference on Nanotechnology (IEEE-NANO) p. 20
[47]	Gao Z Y, Wang K G, Zhang C, Ma H W, Wang G R and Bai J T 2014 Sci. China Technol. Sci. 57 249
[48]	Ma H W, Wang K G, Gao Z Y, Wang H Q, Wang S, Zhang C, Wang G and Bai J 2014 AIP Adv. 4 107139
[49]	Polcari A, Romano P, Sabatino L, Del Vecchio E, Consales M, Cusano A, Cutolo A and Colantuoni V 2011 J. Appl. Phys. 109 074703
[50]	Tang J, Du N and Doyle P S 2011 Proceedings of the National Academy of Sciences 108 16153
[51]	Vasdekis A E, Ońeil C P, Hubbell J A and Psaltis D 2010 Biomacromolecules 11 827
[52]	Marie R, Beech J P, Vörös J, Tegenfeldt J O and Höök F 2006 Langmuir 22 10103
[53]	Liu L and Zhu L Z 2015 Nanoscale Res. Lett. 10 1
[54]	Ren Y K, Yan H, Jiang H Y, Gu J Z and Antonio R 2009 Chin. Phys. B 18 4349
[55]	Wei Q Q, Wei Z J, Ren L M, Zhao H B, Ye T Y, Shi Z J, Fu Y Y, Zhang X and Huang R 2012 Chin. Phys. B 21 088103
[56]	http://enwikipediaorg/wiki/Dielectrophoresis
[57]	Ren Y, Wu H, Feng G, Hou L and Jiang H 2014 Chin. J. Mech. Eng. 27 103
[58]	Smoluchowski M 1918 Naturwissenschaften 6 253
[59]	Hunter R J 1982 J. Colloid Interface Sci. 88 608
[60]	Mannion J, Reccius C, Cross J and Craighead H 2006 Biophys. J. 90 4538
[61]	Han J and Craighead H G 2002 Anal. Chem. 74 394
[62]	Han J, Turner S and Craighead H G 1999 Phys. Rev. Lett. 83 1688
[63]	Yuan Q Z and Zhao Y P 2009 J. Am. Chem. Soc. 131 6374

[1]	Particle captured by a field-modulating vortex through dielectrophoresis force Bing Yan(严兵), Bo Chen(陈波), Zerui Peng(彭泽瑞), and Yong-Liang Xiong(熊永亮). Chin. Phys. B, 2022, 31(3): 034703.
[2]	Effect of Joule heating on the electroosmotic microvortex and dielectrophoretic particle separation controlled by local electric field Bing Yan(严兵), Bo Chen(陈波), Yongliang Xiong(熊永亮), and Zerui Peng(彭泽瑞). Chin. Phys. B, 2021, 30(11): 114701.
[3]	Non-Stokes drag coefficient in single-particle electrophoresis:New insights on a classical problem Mai-Jia Liao(廖麦嘉), Ming-Tzo Wei(魏名佐), Shi-Xin Xu(徐士鑫), H Daniel Ou-Yang(歐陽新喬), Ping Sheng(沈平). Chin. Phys. B, 2019, 28(8): 084701.
[4]	Dynamic resistive switching in a three-terminal device based on phase separated manganites Wang Zhi-Qiang (王志强), Yan Zhi-Bo (颜志波), Qin Ming-Hui (秦明辉), Gao Xing-Sen (高兴森), Liu Jun-Ming (刘俊明). Chin. Phys. B, 2015, 24(3): 037101.
[5]	Vertical assembly of carbon nanotubes for via interconnects Wei Qin-Qin (魏芹芹), Wei Zi-Jun (魏子钧), Ren Li-Ming (任黎明), Zhao Hua-Bo (赵华波), Ye Tian-Yang (叶天扬), Shi Zu-Jin (施祖进), Fu Yun-Yi (傅云义), Zhang Xing (张兴), Huang Ru (黄如). Chin. Phys. B, 2012, 21(8): 088103.
[6]	Microwire formation based on dielectrophoresis of electroless gold plated polystyrene microspheres Jiang Hong-Yuan(姜洪源), Ren Yu-Kun(任玉坤), and Tao Ye(陶冶). Chin. Phys. B, 2011, 20(5): 057701.
[7]	Study on the mixing of fluid in curved microchannelswith heterogeneous surface potentials Lin Jian-Zhong(林建忠), Zhang Kai(张凯), and Li Hui-Jun (李惠君). Chin. Phys. B, 2006, 15(11): 2688-2696.

No Suggested Reading articles found!

Viewed

Full text

Abstract

Cited

Metrics
Related Articles

Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

Cite this article:

Online attention