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Chin. Phys. B, 2010, Vol. 19(4): 049501    DOI: 10.1088/1674-1056/19/4/049501
GEOPHYSICS, ASTRONOMY, AND ASTROPHYSICS Prev  

Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells

Zhuang Zheng-Fei(庄正飞)a), Liu Han-Ping(刘汉平)a), Guo Zhou-Yi(郭周义)a), Zhuo Shuang-Mu(卓双木)b), Yu Bi-Ying(喻碧英) b), and Deng Xiao-Yuan(邓小元)a)†
a MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510631, China; b Key Laboratory of Optoelectronic Science and Technology for Medicine, Ministry of Education, Fujian Normal University, Fuzhou 350007, China
Abstract  This paper first demonstrates second-harmonic generation (SHG) in the intact cell nucleus, which acts as an optical indicator of DNA malignancy in prostate glandular epithelial cells. Within a scanning region of $2.7~\mu$m$\times 2.7~\mu$m in cell nuclei, SHG signals produced from benign prostatic hyperplasia (BPH) and prostate carcinoma (PC) tissues (mouse model C57BL/6) have been investigated. Statistical analyses ($t$ test) of a total of 405 measurements (204 nuclei from BPH and 201 nuclei from PC) show that SHG signals from BPH and PC have a distinct difference ($p < 0.05)$, suggesting a potential optical method of revealing very early malignancy in prostate glandular epithelial cells based upon induced biochemical and/or biophysical modifications in DNA.
Keywords:  nonlinear microscopy      multiharmonic generation      diagnostic applications of nonlinear optics  
Received:  02 January 2009      Revised:  10 October 2009      Accepted manuscript online: 
PACS:  87.14.G- (Nucleic acids)  
  42.65.Ky (Frequency conversion; harmonic generation, including higher-order harmonic generation)  
  87.19.X- (Diseases)  
  87.15.N- (Properties of solutions of macromolecules)  
  87.15.M- (Spectra of biomolecules)  
Fund: Project supported by the National Natural Science Foundation of China (Grant No.~30470495), the Key Laboratory of Optoelectronic Science and Technology for Medicine, Fujian Normal University.

Cite this article: 

Zhuang Zheng-Fei(庄正飞), Liu Han-Ping(刘汉平), Guo Zhou-Yi(郭周义), Zhuo Shuang-Mu(卓双木), Yu Bi-Ying(喻碧英), and Deng Xiao-Yuan(邓小元) Second-harmonic generation as a DNA malignancy indicator of prostate glandular epithelial cells 2010 Chin. Phys. B 19 049501

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