Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers

doi:10.1088/1674-1056/27/6/068701

Abstract Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work, we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K⁺ buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.

Keywords: biophysics G-quadruplexes single-molecule studies DNA structures

Received: 25 December 2017
Revised: 21 March 2018
Accepted manuscript online:

PACS:	87.80.Nj	(Single-molecule techniques)
	87.14.gk	(DNA)
	87.15.H-	(Dynamics of biomolecules)

Fund: Project supported by the National Natural Science Foundation of China (Grant Nos.11474346 and 11774407),the Key Research Program of Frontier Sciences,Chinese Academy of Sciences (Grant No.QYZDB-SSW-SLH045),and the National Key Research and Development Program,China (Grant No.2016YFA0301500).

Corresponding Authors: Peng-Ye Wang, Wei Li
E-mail: pywang@iphy.ac.cn;weili007@iphy.ac.cn

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Yi-Zhou Wang(王一舟), Xi-Miao Hou(侯锡苗), Hai-Peng Ju(车海鹏), Xue Xiao(肖雪), Xu-Guang Xi(奚绪光), Shuo-Xing Dou(窦硕星), Peng-Ye Wang(王鹏业), Wei Li(李伟) Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers 2018 Chin. Phys. B 27 068701

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Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers

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