Abstract To understand the gene-based biological processes in-depth, the single-molecule real-time sequencing has drawn increasing attention with promoted by the Human Genome Project. Herein, a set of newly designed canonical fluorescent bases (A, tC, G, T) are proposed for four-color DNA sequencing. These quasi-intrinsic probes are derived from the fluorophore replacement and ring expansion on natural bases, which still keep the pyrimidine or purine underlying skeleton and Watson-Crick hydrogen bonding face to allow minimal perturbation to the native DNA duplex. More importantly, these nucleobase analogues possess red-shifted absorption and efficient photoluminescence due to the enhanced -conjugation in character. Meanwhile, the four analogues could generate distinct emission wavelength ( nm) for real-time sequencing. To assess the biological employment of the proposed biosensors, the effects of base pairing and linking deoxyribose are also considered.
Fund: Project supported by the National Natural Science Foundation of China (Grant Nos. 11804195, 11847224, 11674198, and 12274265) and the Natural Science Foundation of Shandong Province, China (Grant Nos. ZR2018BA034 and ZR2022MA006).
Yaning Zhang(张雅宁), Yongkang Lyu(吕永康), Zhizheng Cao(曹智正), Xiaolin Chen(陈晓琳), Qingtian Meng(孟庆田), and Changzhe Zhang(张常哲) Design and photophysical characterization of quasi-intrinsic fluorescent probes utilized in DNA sequencing 2025 Chin. Phys. B 34 023203
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