Towards dynamic structure of biological complexes at atomic resolution by cryo-EM

doi:10.1088/1674-1056/27/6/066801

Abstract

Cryo-electron microscopy makes use of transmission electron microscopy to image vitrified biological samples and reconstruct their three-dimensional structures from two-dimensional projections via computational approaches. After over 40 years of development, this technique is now reaching its zenith and reforming the research paradigm of modern structural biology. It has been gradually taking over X-ray crystallography as the mainstream method. In this review, we briefly introduce the history of cryo-EM, recent technical development and its potential power to reveal dynamic structures. The technical barriers and possible approaches to tackle the upcoming challenges are discussed.

Keywords: cryo-electron microscopy protein complexes three-dimensional reconstruction dynamic structures probabilistic conformational spaces

Received: 02 March 2018
Revised: 26 April 2018
Accepted manuscript online:

PACS:

68.37.Lp

(Transmission electron microscopy (TEM))

Corresponding Authors: Kai Zhang
E-mail: kzhang@mrc-lmb.cam.ac.uk

Cite this article:

Kai Zhang(张凯) Towards dynamic structure of biological complexes at atomic resolution by cryo-EM 2018 Chin. Phys. B 27 066801

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Towards dynamic structure of biological complexes at atomic resolution by cryo-EM

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