Reactive oxygen species in plasma against E. coli cells survival rate

doi:10.1088/1674-1056/24/8/085201

Abstract

In this paper, we report on the contrastive analysis of inactivation efficiency of E. coli cells in solution with different disinfection methods. Compared with the hydrogen peroxide solution and the ozone gas, the atmospheric-pressure He plasma can completely kill the E. coli cells in the shortest time. The inactivation efficiency of E. coli cells in solution can be well described by using the chemical reaction rate model. X-ray photoelectron spectroscopy (XPS) analysis shows that the C–O or C=O content of the inactivated E. coli cell surface by plasma is predominantly increased, indicating the quantity of oxygen-containing species in plasma is more than those of two other methods, and then the C–C or C–H bonds can be broken, leading to the etching of organic compounds. Analysis also indicates that plasma-generated species can play a crucial role in the inactivation process by their direct reactions or the decompositions of reactive species, such as ozone into OH radicals in water, then reacting with E. coli cells.

Keywords: atmospheric-pressure plasma reactive oxygen species inactivation efficiency

Received: 09 March 2015
Revised: 09 April 2015
Accepted manuscript online:

PACS:	52.50.Dg	(Plasma sources)
	87.80.-y	(Biophysical techniques (research methods))
	52.77.-j	(Plasma applications)

Fund:

Project supported by the Natural Science Foundation of Fujian Province, China (Grant No. 2014J01025), the National Natural Science Foundation of China (Grant No. 11275261), and the Funds from the Fujian Provincial Key Laboratory for Plasma and Magnetic Resonance, China.

Corresponding Authors: Zhang Xian-Hui
E-mail: zhangxh@xmu.edu.cn

Cite this article:

Zhou Ren-Wu (周仁武), Zhang Xian-Hui (张先徽), Zong Zi-Chao (宗子超), Li Jun-Xiong (李俊雄), Yang Zhou-Bin (杨周斌), Liu Dong-Ping (刘东平), Yang Si-Ze (杨思泽) Reactive oxygen species in plasma against E. coli cells survival rate 2015 Chin. Phys. B 24 085201

[1]	Laroussi M 2002 IEEE Trans. Plasma Sci. 30 1409
[2]	Zhang X H, Liu D P, Song Y, Sun Y and Yang S Z 2013 Phys. Plasma 20 053501
[3]	Kyi M S, Holton J and Ridgway G L 1995 Journal of Hospital Infection 31 275
[4]	A hisharma, Amypruden, Yu Z Q and Collins G J 2005 Environ. Sci. Technol. 39 339
[5]	Xiong Q, Lu X P, Ostrikov K, Xian Y, Zou C, Xiong Z and Pan Y 2010 Phys. Plasmas 17 043506
[6]	Morfill G E, Kong M G and Zimmermann J L 2009 New J. Phys. 11 115011
[7]	Song Y, Liu D P, Ji L F, Wang W C, Zhao P C, Quan C S, Niu J C and Zhang X H 2012 Plasma Process. Polym. 9 17
[8]	Shashurin A, Keidar M, Bronnikov S, Jurjus R A and Stepp M A 2008 Appl. Phys. Lett. 93 181501
[9]	Laroussi M and Leipold F 2004 Int. J. Mass Spectrom. 233 81
[10]	Lu X P, Ye T, Cao Y G, Sun Z Y, Xiong Q, Tang Z Y, Xiong Z L, Hu J, Jiang Z H and Pan Y 2008 J. Appl. Phys. 104 053309
[11]	Locke B and Shih K 2011 Plasma Sources Sci. Technol. 20 034006
[12]	Hofmann J O, Knoll M, Durr M, Schmitt B and Borneff-Lipp M 2005 J. Hospital Infection 59 280
[13]	Khadre M A, Yousef A E and Kim J G 2001 J. Food Sci. 66 1242
[14]	Kim J G, Yousef A E and Dave S 1999 J. Food Protect. 62 1071
[15]	Chen W, Huang J, Du N, et al. 2012 Chin. Phys. Lett. 29 075203
[16]	Huang J, Chen W, Li H, et al. 2013 Acta Phys. Sin. 62 065201 (in Chinese)
[17]	Xiao D, Xie H, Lan Y, et al. 2014 Chin. Phys. B 23 075204
[18]	Bomi G, Kim D B, Moon S Y and Choe W 2009 Curr. Appl. Phys. 9 625
[19]	Xu L, Zhang R, Liu P, Ding L L and Zhan R J 2004 Chin. Phys. 13 913
[20]	Geyter N D, Morent R, Gengembre L, Leys C, Payen E, Vlierberghe S V and Schacht E 2008 Plasma Chem. Plasma P 28 289
[21]	Lu Q Q, Liu D P, Song Y, Zhou R W and Niu J H 2014 Plasma Process. Polym. 11 1028
[22]	Graves D B 2012 J. Phys. D: Appl. Phys. 45 263001
[23]	Zhang X H, Li M J, Zhou R L, Feng K C and Yang S Z 2008 Appl. Phys. Lett. 93 021502
[24]	Zhang X H, Liu D P, Zhou R W, Song Y, Zhang Q, Niu J H, Fan H Y and Yang S Z 2014 Appl. Phys. Lett. 104 043702
[25]	Herbert D, Elsworth R and Telling R C 1956 J. Gen. Microbiol. 14 601
[26]	Hong Z, Cook R D, Davidson D F and Hanson R K 2010 J. Phys. Chem. A 114 5718
[27]	Agostino R D, Cramarossa F, Fracassi F and Illuzzi F 1990 Plasma Deposition, Treatment and Etching of Polymers (San Diego: Acodemic Press) pp. 95–162
[28]	Geyter N D, Morent R, Leys C, Gengembre L, Payen E, Vlierberghe S V and Schacht E 2008 Surf. Coat. Technol. 202 3000
[29]	Niu J, Liu D, Chen J, Ding H and Wu Y 2012 Eur. Phys. J. Appl. Phys. 57 10801
[30]	Chan C M, Ko T M and Hiraoka H 1996 Surf. Sci. Rep. 24 1
[31]	Khan F U, Rehman N U, Naseer S, Naveed M A, Qayyum A, Khattak N A D and Zakaullah M 2009 Eur. Phys. J. Appl. Phys. 45 11002
[32]	Sakamoto T, Matsuura H and Akatsuka H 2007 J. Appl. Phys. 101 23307
[33]	Sarani A, Nikiforov Y A and Leys C 2010 Phys. Plasmas 17 063504
[34]	Liu D, Niu J and Yu N 2011 J. Vac. Sci. Technol. A 29 061506
[35]	Zhang X, Liu D, Wang H, Liu L, Wang S and Yang S 2012 Plasma Chem. Plasma P 32 949
[36]	Zhou W, Li Q, Zhu X and Pu Y 2009 J. Phys: D: Appl. Phys. 42 202002
[37]	Golubovskii Y B, Maiorov V A, Behnke J and Behnke J F 2003 J. Phys: D: Appl. Phys. 36 39
[38]	Karaca H and Velioglu Y S 2007 Food Rev. Int. 23 91

[1]	LIF diagnostics of hydroxyl radical in a methanol containing atmospheric-pressure plasma jet Mu-Yang Qian(钱沐杨), San-Qiu Liu(刘三秋), Xue-Kai Pei(裴学凯), Xin-Pei Lu(卢新培), Jia-Liang Zhang(张家良), De-Zhen Wang(王德真). Chin. Phys. B, 2016, 25(10): 105205.
[2]	Toxicity of superparamagnetic iron oxide nanoparticles：Research strategies and implications for nanomedicine Li Lei (李蕾), Jiang Ling-Ling (江玲玲), Zeng Yun (曾云), Liu Gang (刘刚). Chin. Phys. B, 2013, 22(12): 127503.

No Suggested Reading articles found!

Viewed

Full text

Abstract

Cited

Metrics
Related Articles

Reactive oxygen species in plasma against E. coli cells survival rate

Cite this article:

Online attention