Reflection-mode photoacoustic microscopy using a hollow focused ultrasound transducer for in vivo imaging of blood vessels

doi:10.1088/1674-1056/21/5/054211

Abstract A reflection-mode photoacoustic microscope using a hollow focused ultrasound transducer is developed for high-resolution in vivo imaging. A confocal structure of the laser and the ultrasound is used to improve the system resolution. The axial and lateral resolutions of the system are measured to be ～ 32 μm and ～ 58 μm, respectively. Ex vivo and in vivo modes are tested to validate the imaging capability of the photoacoustic microscope. The adjacent vein and artery can be seen clearly from the reconstructed photoacoustic images. The results demonstrate that the reflection-mode photoacoustic microscope can be used for high-resolution imaging of micro-blood vessels, which would be of great benefit for monitoring the neovascularization in tumor angiogenesis.

Keywords: photoacoustic microscopy hollow focused ultrasound transducer blood vessels

Received: 22 October 2011
Revised: 27 April 2012
Accepted manuscript online:

PACS:

42.62.-b

(Laser applications)

Fund: Project supported by the National Basic Research Program of China (Grant Nos. 2010CB732602 and 2011CB910402), the Program for Changjiang Scholars and Innovative Research Team in University, China (Grant No. IRT0829), the National Natural Science Foundation of China (Grant Nos. 30870676, 81127004 and 11104087), and the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (Grant No. LYM10061).

Cite this article:

Yuan Yi(袁毅) and Yang Si-Hua(杨思华) Reflection-mode photoacoustic microscopy using a hollow focused ultrasound transducer for in vivo imaging of blood vessels 2012 Chin. Phys. B 21 054211

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Reflection-mode photoacoustic microscopy using a hollow focused ultrasound transducer for in vivo imaging of blood vessels

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