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Chin. Phys. B, 2012, Vol. 21(5): 054211    DOI: 10.1088/1674-1056/21/5/054211
ELECTROMAGNETISM, OPTICS, ACOUSTICS, HEAT TRANSFER, CLASSICAL MECHANICS, AND FLUID DYNAMICS Prev   Next  

Reflection-mode photoacoustic microscopy using a hollow focused ultrasound transducer for in vivo imaging of blood vessels

Yuan Yi(袁毅) and Yang Si-Hua(杨思华)
MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China
Abstract  A reflection-mode photoacoustic microscope using a hollow focused ultrasound transducer is developed for high-resolution in vivo imaging. A confocal structure of the laser and the ultrasound is used to improve the system resolution. The axial and lateral resolutions of the system are measured to be ~ 32 μm and ~ 58 μm, respectively. Ex vivo and in vivo modes are tested to validate the imaging capability of the photoacoustic microscope. The adjacent vein and artery can be seen clearly from the reconstructed photoacoustic images. The results demonstrate that the reflection-mode photoacoustic microscope can be used for high-resolution imaging of micro-blood vessels, which would be of great benefit for monitoring the neovascularization in tumor angiogenesis.
Keywords:  photoacoustic microscopy      hollow focused ultrasound transducer      blood vessels  
Received:  22 October 2011      Revised:  27 April 2012      Accepted manuscript online: 
PACS:  42.62.-b (Laser applications)  
Fund: Project supported by the National Basic Research Program of China (Grant Nos. 2010CB732602 and 2011CB910402), the Program for Changjiang Scholars and Innovative Research Team in University, China (Grant No. IRT0829), the National Natural Science Foundation of China (Grant Nos. 30870676, 81127004 and 11104087), and the Foundation for Distinguished Young Talents in Higher Education of Guangdong, China (Grant No. LYM10061).

Cite this article: 

Yuan Yi(袁毅) and Yang Si-Hua(杨思华) Reflection-mode photoacoustic microscopy using a hollow focused ultrasound transducer for in vivo imaging of blood vessels 2012 Chin. Phys. B 21 054211

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