Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method

doi:10.1088/1674-1056/20/1/010704

Abstract We successfully conduct the label-free and real-time detection of the interactions between epoxy groups and rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide, and between the hybridization of 5' CTT CAG GTC ATG AGC CTG AT 3' and its complementary 3' GAA GTC CAG TAC TCG GAC TA 5' oligonucleotide, by the oblique-incidence reflectivity difference (OI-RD) method. The dynamic curves of OI-RD signals, corresponding to the kinetic processes of biomolecular combination or hybridization, are acquired. In our case, the combination of epoxy groups with rabbit IgG and 5' CTT CAG GTC ATG AGC CTG AT 3' oligonucleotide need almost one and a half hours and about two hundred seconds, respectively; and the hybridization of the two oligonucleotides needs about five hundred seconds. The experimental results show that the OI-RD is a promising method for the real-time and label-free detection of biomolecular interactions.

Keywords: label-free detection OI-RD biomolecular reactions

Received: 02 February 2010
Revised: 26 February 2010
Accepted manuscript online:

PACS:	07.60.Fs	(Polarimeters and ellipsometers)
	81.70Fy
	87.80Dj

Fund: Project supported by the National Basic Research Program in China (Grant No. 2007CB935700).

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Wang Xu(王旭), Lu Heng(陆珩), Dai Jun(戴俊), Wen Juan(温娟), Yuan Kun(原昆), Lü Hui-Bin(吕惠宾), Jin Kui-Juan(金奎娟), Zhou Yue-Liang(周岳亮), and Yang Guo-Zhen(杨国桢) Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method 2011 Chin. Phys. B 20 010704

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Real-time and label-free detection of biomolecular interactions by oblique-incidence reflectivity difference method

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