Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy

doi:10.1088/1674-1056/22/7/076801

中国物理B ›› 2013, Vol. 22 ›› Issue (7): 76801-076801.doi: 10.1088/1674-1056/22/7/076801

• CONDENSED MATTER: STRUCTURAL, MECHANICAL, AND THERMAL PROPERTIES • 上一篇下一篇

Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy

张凯^a, 李德娥^b, 洪友丽^a, 朱佩平^a, 袁清习^a, 黄万霞^a, 高昆^c, 周红章^b, 吴自玉^{a c}

a Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;
b Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;
c National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230026, China

收稿日期:2012-12-25 修回日期:2013-02-26 出版日期:2013-06-01 发布日期:2013-06-01
基金资助:
Project supported by the National Basic Research Program of China (Grant Nos. 2012CB825800 and 2009CB930804), the Key Program of the National Natural Science Foundation of China (Grant Nos. 10734070 and 10774144), the National Natural Science Foundation of China (Grant Nos. 11205189, 10979055, 31071909, and 31272358), and the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KJCX2-YW-N42).

Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy

Zhang Kai (张凯)^a, Li Dee (李德娥)^b, Hong You-Li (洪友丽)^a, Zhu Pei-Ping (朱佩平)^a, Yuan Qing-Xi (袁清习)^a, Huang Wan-Xia (黄万霞)^a, Gao Kun (高昆)^c, Zhou Hong-Zhang (周红章)^b, Wu Zi-Yu (吴自玉)^{a c}

a Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049, China;
b Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China;
c National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei 230026, China

Received:2012-12-25 Revised:2013-02-26 Online:2013-06-01 Published:2013-06-01
Contact: Zhou Hong-Zhang, Wu Zi-Yu E-mail:zhouhz@ioz.ac.cn;wuzy@ustc.edu.cn
Supported by:
Project supported by the National Basic Research Program of China (Grant Nos. 2012CB825800 and 2009CB930804), the Key Program of the National Natural Science Foundation of China (Grant Nos. 10734070 and 10774144), the National Natural Science Foundation of China (Grant Nos. 11205189, 10979055, 31071909, and 31272358), and the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No. KJCX2-YW-N42).

摘要/Abstract

摘要： A penetrating view of the three-dimensional nano-structure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscope are quite promising for insect morphology studies, in particularly, provide an attractive alternative to destructive techniques for internal soft tissues.

关键词: X-ray microscopy, computed tomography, synchrotron radiation source, morphology

Abstract: A penetrating view of the three-dimensional nano-structure of female spermatheca and male flagellum in the species Aleochara verna is obtained with 100-nm resolution using a hard X-ray microscope, which provides a fast noninvasive imaging technology for insect morphology. Through introducing Zernike phase contrast and heavy metal staining, images taken at 8 keV displayed sufficient contrast for observing nanoscale fine structures, such as the spermatheca cochleate duct and the subapex of the flagellum, which have some implications for the study of the sperm transfer process and genital evolution in insects. This work shows that both the spatial resolution and the contrast characteristic of hard X-ray microscope are quite promising for insect morphology studies, in particularly, provide an attractive alternative to destructive techniques for internal soft tissues.

Key words: X-ray microscopy, computed tomography, synchrotron radiation source, morphology

中图分类号: (X-ray microscopy)

68.37.Yz

81.70.Tx (Computed tomography) 07.85.Qe (Synchrotron radiation instrumentation) 87.16.Mq (Morphology of nerve cells)

张凯, 李德娥, 洪友丽, 朱佩平, 袁清习, 黄万霞, 高昆, 周红章, 吴自玉. Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy[J]. 中国物理B, 2013, 22(7): 76801-076801.

Zhang Kai (张凯), Li Dee (李德娥), Hong You-Li (洪友丽), Zhu Pei-Ping (朱佩平), Yuan Qing-Xi (袁清习), Huang Wan-Xia (黄万霞), Gao Kun (高昆), Zhou Hong-Zhang (周红章), Wu Zi-Yu (吴自玉). Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy[J]. Chin. Phys. B, 2013, 22(7): 76801-076801.

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Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy

Penetrating view of nano-structures in Aleochara verna spermatheca and flagellum by hard X-ray microscopy

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