中国物理B ›› 2025, Vol. 34 ›› Issue (12): 128703-128703.doi: 10.1088/1674-1056/ade424

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Tumor cell directed migration based on 3D printed microfilament structure

Dongtian Zheng(郑栋天)1, Zhikai Ye(叶志凯)2, Chuyun Wang(汪楚云)4, Lianjie Zhou(周连杰)1, Xiyao Yao(姚喜耀)1, Guoqiang Li(李国强)3, Guo Chen(陈果)1,†, and Liyu Liu(刘雳宇)1,‡   

  1. 1 Chongqing Key Laboratory of Interface Physics in Energy Conversion, School of Physics and Center of Quantum Materials and Devices, Chongqing University, Chongqing 401331, China;
    2 Center for Theoretical Interdisciplinary Sciences Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China;
    3 College of Chemistry & Environmental Engineering, Chongqing University of Arts and Sciences, Yongchuan 402160, China;
    4 Postgraduate Training Base Alliance, Wenzhou Medical University, Wenzhou 325035, China
  • 收稿日期:2025-03-28 修回日期:2025-06-03 接受日期:2025-06-13 发布日期:2025-12-10
  • 通讯作者: Guo Chen, Liyu Liu E-mail:wezer@cqu.edu.cn;lyliu@cqu.edu.cn
  • 基金资助:
    Project supported by the National Natural Science Foundation of China (Grant Nos. T2350007 and 12174041), the Natural Science Foundation of Chongqing (Grant No. CSTB2024NSCQ-MSX0596), the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJZD-K202401306), and the Scientific Research Fund of Chongqing University of Arts and Sciences (Grant Nos. R2023HH03 and P2022HH05).

Tumor cell directed migration based on 3D printed microfilament structure

Dongtian Zheng(郑栋天)1, Zhikai Ye(叶志凯)2, Chuyun Wang(汪楚云)4, Lianjie Zhou(周连杰)1, Xiyao Yao(姚喜耀)1, Guoqiang Li(李国强)3, Guo Chen(陈果)1,†, and Liyu Liu(刘雳宇)1,‡   

  1. 1 Chongqing Key Laboratory of Interface Physics in Energy Conversion, School of Physics and Center of Quantum Materials and Devices, Chongqing University, Chongqing 401331, China;
    2 Center for Theoretical Interdisciplinary Sciences Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China;
    3 College of Chemistry & Environmental Engineering, Chongqing University of Arts and Sciences, Yongchuan 402160, China;
    4 Postgraduate Training Base Alliance, Wenzhou Medical University, Wenzhou 325035, China
  • Received:2025-03-28 Revised:2025-06-03 Accepted:2025-06-13 Published:2025-12-10
  • Contact: Guo Chen, Liyu Liu E-mail:wezer@cqu.edu.cn;lyliu@cqu.edu.cn
  • Supported by:
    Project supported by the National Natural Science Foundation of China (Grant Nos. T2350007 and 12174041), the Natural Science Foundation of Chongqing (Grant No. CSTB2024NSCQ-MSX0596), the Science and Technology Research Program of Chongqing Municipal Education Commission (Grant No. KJZD-K202401306), and the Scientific Research Fund of Chongqing University of Arts and Sciences (Grant Nos. R2023HH03 and P2022HH05).

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摘要： Three-dimensional (3D) cell spheroids, generated utilizing the self-organizing properties of mammalian cells, exhibit significant advantages and hold important value in simulating tissue complexity. However, they still encounter numerous limitations, including the absence of spatial anisotropy in cell spheroids, which can compromise their reliability in numerous preclinical drug tests. This study utilizes two-photon polymerization (TPP) 3D printing technology, drawing inspiration from common liquid transport structures in nature, to design a microstructure featuring periodic parallel microcavities and wedge angles. This design enables unilateral immobilization and capillary rise of soft condensed matter. This structure facilitates the directed migration of 3D cell spheroids through the physical properties of the structure itself in static culture. Consequently, the original 3D cultured cell spheroids can acquire unique anisotropy within the spatial structure in a static culture environment, presenting a novel perspective for constructing biological constructs and cultivating connections between various cell spheroids, such as organoids.

关键词: cell migration, laplace pressure, corner effect, TPP 3D printing

Abstract: Three-dimensional (3D) cell spheroids, generated utilizing the self-organizing properties of mammalian cells, exhibit significant advantages and hold important value in simulating tissue complexity. However, they still encounter numerous limitations, including the absence of spatial anisotropy in cell spheroids, which can compromise their reliability in numerous preclinical drug tests. This study utilizes two-photon polymerization (TPP) 3D printing technology, drawing inspiration from common liquid transport structures in nature, to design a microstructure featuring periodic parallel microcavities and wedge angles. This design enables unilateral immobilization and capillary rise of soft condensed matter. This structure facilitates the directed migration of 3D cell spheroids through the physical properties of the structure itself in static culture. Consequently, the original 3D cultured cell spheroids can acquire unique anisotropy within the spatial structure in a static culture environment, presenting a novel perspective for constructing biological constructs and cultivating connections between various cell spheroids, such as organoids.

Key words: cell migration, laplace pressure, corner effect, TPP 3D printing

中图分类号:  (Mechanical and micromechanical techniques)

  • 87.80.Ek
68.03.Cd (Surface tension and related phenomena) 47.55.dr (Interactions with surfaces) 87.85.Va (Micromachining)