Influence of matrix-metalloproteinase inhibitor on the interaction between cancer cells and matrigel

doi:10.1088/1674-1056/ab85c1

中国物理B ›› 2020, Vol. 29 ›› Issue (6): 68701-068701.doi: 10.1088/1674-1056/ab85c1

• SPECIAL TOPIC—Recent advances in thermoelectric materials and devices • 上一篇下一篇

Influence of matrix-metalloproteinase inhibitor on the interaction between cancer cells and matrigel

Teng Ye(叶腾), Fangfu Ye(叶方富), Feng Qiu(邱峰)

1 First Clinical Medical College, Nanchang University, Nanchang 330006, China;
2 Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
3 Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China;
4 Department of Oncology, First Affiliated Hospital of Nanchang University, Nanchang 330006, China

收稿日期:2020-02-14 修回日期:2020-03-31 出版日期:2020-06-05 发布日期:2020-06-05
通讯作者: Fangfu Ye, Feng Qiu E-mail:fye@iphy.ac.cn;lukeqiubmu@163.com
基金资助:
Project supported by the National Natural Science Foundation of China (Grant No. 11774394), the Key Research Program of Frontier Sciences of Chinese Academy of Sciences (Grant No. QYZDB-SSW-SYS003), and the K.C. Wong Education Foundation.

Influence of matrix-metalloproteinase inhibitor on the interaction between cancer cells and matrigel

Teng Ye(叶腾)¹, Fangfu Ye(叶方富)^2,3, Feng Qiu(邱峰)^1,4

1 First Clinical Medical College, Nanchang University, Nanchang 330006, China;
2 Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
3 Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou 325001, China;
4 Department of Oncology, First Affiliated Hospital of Nanchang University, Nanchang 330006, China

Received:2020-02-14 Revised:2020-03-31 Online:2020-06-05 Published:2020-06-05
Contact: Fangfu Ye, Feng Qiu E-mail:fye@iphy.ac.cn;lukeqiubmu@163.com
Supported by:
Project supported by the National Natural Science Foundation of China (Grant No. 11774394), the Key Research Program of Frontier Sciences of Chinese Academy of Sciences (Grant No. QYZDB-SSW-SYS003), and the K.C. Wong Education Foundation.

摘要/Abstract

摘要： Various behaviors of cancer cells are strongly influenced by their interaction with extracellular matrices (ECM). We investigate how this interaction may be influenced if the cancer cells' ability of secreting matrix metalloproteinases (MMPs) to degrade ECM is inhibited by adding the MMP inhibitor. We use MDA-MB-231-GFP cells as model cells and use matrigel to mimic ECM. It is found that the added MMP inhibitor significantly reduces the migration speed of cancer cells covered by matrigel but has little influence on the migration persistence and shape factor of the cells and that with the MMP inhibitor added the presence of matrigel on the top has no influence on the migration speed of the cells but increases the cells' shape factor and migration persistence.

关键词: cancer cells, matrigel, matrix metalloproteinase (MMP) inhibitor, migration

Abstract: Various behaviors of cancer cells are strongly influenced by their interaction with extracellular matrices (ECM). We investigate how this interaction may be influenced if the cancer cells' ability of secreting matrix metalloproteinases (MMPs) to degrade ECM is inhibited by adding the MMP inhibitor. We use MDA-MB-231-GFP cells as model cells and use matrigel to mimic ECM. It is found that the added MMP inhibitor significantly reduces the migration speed of cancer cells covered by matrigel but has little influence on the migration persistence and shape factor of the cells and that with the MMP inhibitor added the presence of matrigel on the top has no influence on the migration speed of the cells but increases the cells' shape factor and migration persistence.

Key words: cancer cells, matrigel, matrix metalloproteinase (MMP) inhibitor, migration

中图分类号: (Cell processes)

87.17.-d

87.17.Jj (Cell locomotion, chemotaxis) 87.18.Ed (Cell aggregation) 05.40.Jc (Brownian motion)

叶腾, 叶方富, 邱峰. Influence of matrix-metalloproteinase inhibitor on the interaction between cancer cells and matrigel[J]. 中国物理B, 2020, 29(6): 68701-068701.

Teng Ye(叶腾), Fangfu Ye(叶方富), Feng Qiu(邱峰). Influence of matrix-metalloproteinase inhibitor on the interaction between cancer cells and matrigel[J]. Chin. Phys. B, 2020, 29(6): 68701-068701.

参考文献 26

[1]	Weidner N, Folkman J, Pozza F, Bevilacqua P, Allred E N, Moore D H, Meli S and Gasparini G 1992 J. Natl. Cancer Inst. 84 1875 doi: 10.1093/jnci/84.24.1875
[2]	Spaeth E L, Dembinski J L, Sasser A K, Watson K, Klopp A, Hall B, Andreeff M and Marini F 2009 PLoS One 4 e4992
[3]	Whiteside T L 2008 Oncogene 27 5904 doi: 10.1038/onc.2008.271
[4]	Rejniak K A 2016 Systems Biology of Tumor Microenvironment: Quantitative Modeling and Simulations (Berlin: Springer Int Publishing AG) pp. 73-91
[5]	Hockel M and Vaupel P 2001 JNCI-J. Natl. Cancer Inst. 93 266 doi: 10.1093/jnci/93.4.266
[6]	Xu R, Boudreau A and Bissell M 2009 Cancer Metastasis Rev. 28 167 doi: 10.1007/s10555-008-9178-z
[7]	Provenzano P P, Inman D R, Eliceiri K W, Knittel J G, Yan L, Rueden C T, White J G and Keely P J 2008 BMC Med. 6 11 doi: 10.1186/1741-7015-6-11
[8]	Levental K R, Yu H, Kass L, Lakins J N, Egeblad M, Erler J T, Fong S F T, Csiszar K, Giaccia A, Weninger W, Yamauchi M, Gasser D L and Weaver V M 2009 Cell 139 891 doi: 10.1016/j.cell.2009.10.027
[9]	Rowe R G and Weiss S J 2008 Trends Cell Biol. 18 560 doi: 10.1016/j.tcb.2008.08.007
[10]	Kalluri R 2003 Nat. Rev. Cancer 3 422 doi: 10.1038/nrc1094
[11]	Kleinman H K and Martin G R 2005 Semin. Cancer Biol. 15 378 doi: 10.1016/j.semcancer.2005.05.004
[12]	Hughes C S, Postovit L M and Lajoie G A 2010 Proteomics 10 1886 doi: 10.1002/pmic.200900758
[13]	Price K J, Tsykin A, Giles K M, Sladic R T, Epis M R, Ganss R, Goodall G J and Leedman P J 2012 Biochem. Biophys. Res. Commun. 427 343 doi: 10.1016/j.bbrc.2012.09.059
[14]	Badea M A, Balas M, Hermenean A, Ciceu A, Herman H, Ionita D and Dinischiotu A 2019 SLAS Discovery: Advancing Life Sciences R & D 24 563 doi: Discovery: Advancing Life Sciences R
[15]	Ye T and Qiu F 2019 Chin. Phys. B 28 108704 doi: 10.1088/1674-1056/ab4275
[16]	Coussens L M and Werb Z 1996 Chem. Biol. 3 895 doi: 10.1016/S1074-5521(96)90178-7
[17]	Hanahan D and Weinberg R A 2000 Cell 100 57 doi: 10.1016/S0092-8674(00)81683-9
[18]	Garbett E A, Reed M W R and Brown N J 1999 British J. Cancer 81 287 doi: 10.1038/sj.bjc.6690689
[19]	Pacheco M M, Mourao M, Mantovani E B, Nishimoto I N and Brentani M M 1998 Clin. Exp. Metastasis 16 577 doi: 10.1023/A:1006580415796
[20]	Piccinini F, Kiss A and Horvath P 2016 Bioinformatics 32 955 doi: 10.1093/bioinformatics/btv686
[21]	Gorelik R and Gautreau A 2014 Nat. Protoc. 9 1931 doi: 10.1038/nprot.2014.131
[22]	Park J A, Kim J H, Bi D et al. 2015 Nat. Mater. 14 1040 doi: 10.1038/nmat4357
[23]	Boromand A, Signoriello A, Ye F, O'Hern C S and Shattuck M D 2018 Phys. Rev. Lett. 121 248003 doi: 10.1103/PhysRevLett.121.248003
[24]	Liu Y P, Zhang X C, Wu Y L, Liu W, Li X, Liu R C, Liu L Y and Shuai J W 2017 Chin. Phys. B 26 128707 doi: 10.1088/1674-1056/26/12/128707
[25]	Diao W, Tong X, Yang C, Zhang F, Bao C, Chen H, Liu L, Li M, Ye F, Fan Q, Wang J and Ou-Yang Z C 2019 Sci. Rep. 9 85 doi: 10.1038/s41598-018-36347-7
[26]	Zheng Y, Nan H, Liu Y, Fan Q, Wang X, Liu R, Liu L, Ye F, Sun B and Jiao Y 2019 Phys. Rev. E 100 043303 doi: 10.1103/PhysRevE.100.043303

Influence of matrix-metalloproteinase inhibitor on the interaction between cancer cells and matrigel

Influence of matrix-metalloproteinase inhibitor on the interaction between cancer cells and matrigel

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献 26

相关文章 15

Metrics

本文评价

推荐阅读 0

[1]	Yuhui Dong(董宇辉), Danni Yan(严丹妮), Shuai Yang(杨帅), Naiwei Wei(魏乃炜),Yousheng Zou(邹友生), and Haibo Zeng(曾海波). Ion migration in metal halide perovskite QLEDs and its inhibition[J]. 中国物理B, 2023, 32(1): 18507-018507.
[2]	Pei-Feng Lin(林培锋), Xiao Hu(胡箫), and Jian-Zhong Lin(林建忠). Inertial focusing and rotating characteristics of elliptical and rectangular particle pairs in channel flow[J]. 中国物理B, 2022, 31(8): 80501-080501.
[3]	Zhi-Yong Ding(丁智勇), Pan-Feng Zhou(周攀峰), Xiao-Gang Fan(范小刚),Cheng-Cheng Liu(刘程程), Juan He(何娟), and Liu Ye(叶柳). Coherence migration in high-dimensional bipartite systems[J]. 中国物理B, 2022, 31(6): 60308-060308.
[4]	Yanping Liu(刘艳平), Da He(何达), Yang Jiao(焦阳), Guoqiang Li(李国强), Yu Zheng(郑钰), Qihui Fan(樊琪慧), Gao Wang(王高), Jingru Yao(姚静如), Guo Chen(陈果), Silong Lou(娄四龙), and Liyu Liu(刘雳宇). Nonlinear dynamics of cell migration in anisotropic microenvironment[J]. 中国物理B, 2021, 30(9): 90505-090505.
[5]	Xiaochen Wang(王晓晨), Qihui Fan (樊琪慧), and Fangfu Ye(叶方富). Migration and shape of cells on different interfaces[J]. 中国物理B, 2021, 30(9): 90502-090502.
[6]	Mingrui He(何明睿), Yang Fan(樊洋), Yueming Zhou(周月明), and Peixiang Lu(陆培祥). Taking snapshots of a moving electron wave packet in molecules using photoelectron holography in strong-field tunneling ionization[J]. 中国物理B, 2021, 30(12): 123202-123202.
[7]	Ru-Qi Li(李汝琦), Yu-Rong Song(宋玉蓉), and Guo-Ping Jiang(蒋国平). Prediction of epidemics dynamics on networks with partial differential equations: A case study for COVID-19 in China[J]. 中国物理B, 2021, 30(12): 120202-120202.
[8]	Xue-Yan Wang(王雪岩), Hu Wang(王虎), Luo-Ran Chen(陈烙然), Yu-Chuan Shao(邵宇川), and Jian-Da Shao(邵建达). Suppression of ion migration in perovskite materials by pulse-voltage method[J]. 中国物理B, 2021, 30(11): 118104-118104.
[9]	张学阳, 王昆, 陈军, 胡望宇, 祝文军, 肖时芳, 邓辉球, 蔡孟秋. Shock-induced migration of asymmetry tilt grain boundary in iron bicrystal: A case study of Σ3 [110][J]. 中国物理B, 2019, 28(12): 126201-126201.
[10]	叶腾, 邱峰. Influence of matrigel on the shape and dynamics of cancer cells[J]. 中国物理B, 2019, 28(10): 108704-108704.
[11]	刘铁, 王强, 苑轶, 王凯, 李国建. High-gradient magnetic field-controlled migration of solutes and particles and their effects on solidification microstructure: A review[J]. 中国物理B, 2018, 27(11): 118103-118103.
[12]	尹元枫, 邢辉, 臧渡洋, 金克新. Effect of elasticity mismatch on cell deformation and migration: A phase-field study[J]. 中国物理B, 2018, 27(11): 116201-116201.
[13]	陈杰, 黄溥曼, 韩小标, 潘郑州, 钟昌明, 梁捷智, 吴志盛, 刘扬, 张佰君. Influence of adatom migration on wrinkling morphologies of AlGaN/GaN micro-pyramids grown by selective MOVPE[J]. 中国物理B, 2017, 26(6): 68101-068101.
[14]	钟睿, 侯氢, 马超琼, 付宝勤, 汪俊. Temperature dependence of migration features of self-interstitials in zirconium[J]. 中国物理B, 2017, 26(12): 120202-120202.
[15]	刘艳平, 张晓翠, 吴宇宁, 刘雯, 李翔, 刘如川, 刘雳宇, 帅建伟. Derivation of persistent time for anisotropic migration of cells[J]. 中国物理B, 2017, 26(12): 128707-128707.