中国物理B ›› 2000, Vol. 9 ›› Issue (11): 855-860.doi: 10.1088/1009-1963/9/11/012

• • 上一篇    

CONSTRUCTION OF AN OPTICAL TWEEZERS—CALCULATION AND EXPERIMENTS

高崇明1, 孙巍2, 王义遒2   

  1. (1)College of Life Science, Peking University, Beijing 100871, China; (2)Department of Electronics, Peking University, Beijing 100871, China
  • 收稿日期:2000-04-03 修回日期:2000-06-07 出版日期:2000-11-15 发布日期:2005-06-10

CONSTRUCTION OF AN OPTICAL TWEEZERS—CALCULATION AND EXPERIMENTS

Sun Wei (孙巍)a, Wang Yi-qiu (王义遒)a, Gao Chong-ming (高崇明)b   

  1. a Department of Electronics, Peking University, Beijing 100871, China; b College of Life Science, Peking University, Beijing 100871, China
  • Received:2000-04-03 Revised:2000-06-07 Online:2000-11-15 Published:2005-06-10

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摘要： We report the construction of an optical tweezers system and the results of the trapping and the moving of living algal cells. Experimental study on trapping polystyrene spheres demonstrates the effects of the system parameters on the trap. The trapping forces on dielectric spheres are calculated using Ashkin's ray-optics model. The result shows that an objective with higher numerical aperture can build a more stable trap along axial direction than an objective with lower numerical aperture, though their transverse trapping forces are on the same order.

Abstract: We report the construction of an optical tweezers system and the results of the trapping and the moving of living algal cells. Experimental study on trapping polystyrene spheres demonstrates the effects of the system parameters on the trap. The trapping forces on dielectric spheres are calculated using Ashkin's ray-optics model. The result shows that an objective with higher numerical aperture can build a more stable trap along axial direction than an objective with lower numerical aperture, though their transverse trapping forces are on the same order.

Key words: optical tweezers, light force, cell biology

中图分类号:  (Cell processes)

  • 87.17.-d
87.64.M- (Optical microscopy) 87.80.Cc (Optical trapping)