Twisting mode of supercoil leucine-rich domain mediates peptide sensing in FLS2–flg22–BAK1 complex

doi:10.1088/1674-1056/abaee1

中国物理B ›› 2020, Vol. 29 ›› Issue (10): 108709-.doi: 10.1088/1674-1056/abaee1

所属专题： SPECIAL TOPIC — Modeling and simulations for the structures and functions of proteins and nucleic acids

Zhi-Chao Liu(刘志超)¹^,³, Qin Liu(刘琴)⁴, Chan-You Chen(陈禅友)⁴, Chen Zeng(曾辰)³, Peng Ran(冉鹏)¹^,³, Yun-Jie Zhao(赵蕴杰)²^,†(), Lei Pan(潘磊)⁴^,‡()

收稿日期:2020-06-23 修回日期:2020-08-07 接受日期:2020-08-13 出版日期:2020-10-05 发布日期:2020-10-05
通讯作者: Yun-Jie Zhao(赵蕴杰), Lei Pan(潘磊)

Twisting mode of supercoil leucine-rich domain mediates peptide sensing in FLS2–flg22–BAK1 complex

Zhi-Chao Liu(刘志超)^1,3, Qin Liu(刘琴)⁴, Chan-You Chen(陈禅友)⁴, Chen Zeng(曾辰)³, Peng Ran(冉鹏)^1,3, Yun-Jie Zhao(赵蕴杰)^2,†, and Lei Pan(潘磊)^4,‡

¹ School of Biological Information, Chongqing University of Posts and Telecommunications, Chongqing 400065, China
² Institute of Biophysics and Department of Physics, Central China Normal University, Wuhan 430079, China
³ Department of Physics, The George Washington University, Washington, DC, 20052, USA
⁴ School of Life Sciences, Jianghan University, Wuhan 430056, China

Received:2020-06-23 Revised:2020-08-07 Accepted:2020-08-13 Online:2020-10-05 Published:2020-10-05
Contact: ^†Corresponding author. E-mail: yjzhaowh@mail.ccnu.edu.cn ^‡Corresponding author. E-mail: leipan@jhun.edu.cn
About author:
†Corresponding author. E-mail: yjzhaowh@mail.ccnu.edu.cn
‡Corresponding author. E-mail: leipan@jhun.edu.cn
* Project supported by the National Natural Science Foundation of China (Grant No. 11704140), self-determined research funds of CCNU from the Colleges’ Basic Research and Operation of MOE (Grant No. CCNU20TS004) (Y. Z.), and the China Scholarship Council Fund (Grant No. 201708420039) (L. P.).

摘要/Abstract

Abstract:

Plants and animals recognize microbial invaders by detecting pathogen-associated molecular patterns (PAMPs) through pattern-recognition receptors (PRRs). This recognition plays a crucial role in plant immunity. The newly discovered protein in plants that responds to bacterial flagellin, i.e., flagellin-sensitive 2 (FLS2), is ubiquitously expressed and present in many plants. The association of FLS2 and BAK1, facilitated by a highly conserved epitope flg22 of flagellin, triggers such downstream immune responses as activated MAPK pathway and elevated reactive oxygen species (ROS) for bacterial defense and plant immunity. Here we study the intrinsic dynamics and conformational change of FLS2 upon the formation of the FLS2–flg22–BAK1 complex. The top intrinsic normal modes and principal structural fluctuation components are very similar, showing two bending modes and one twisting mode. The twisting mode alone, however, accounts for most of the conformational change of FLS2 induced by binding with flg22 and BAK1. This study indicates that flg22 binding suppresses FLS2 conformational fluctuation, especially on the twisting motion, thus facilitating FLS2–BAK1 interaction. A detailed analysis of this sensing mechanism may aid better design on both PRR and peptide mimetics for plant immunity.

Key words: plant immunity, flagellin-sensitive 2, peptide sensing mechanism

中图分类号: (RNA)

87.14.gn

87.15.K- (Molecular interactions; membrane-protein interactions) 87.10.Ca (Analytical theories) 87.15.A- (Theory, modeling, and computer simulation)

Zhi-Chao Liu(刘志超), Qin Liu(刘琴), Chan-You Chen(陈禅友), Chen Zeng(曾辰), Peng Ran(冉鹏), Yun-Jie Zhao(赵蕴杰), Lei Pan(潘磊). [J]. 中国物理B, 2020, 29(10): 108709-.

Zhi-Chao Liu(刘志超), Qin Liu(刘琴), Chan-You Chen(陈禅友), Chen Zeng(曾辰), Peng Ran(冉鹏), Yun-Jie Zhao(赵蕴杰)†, and Lei Pan(潘磊)‡. Twisting mode of supercoil leucine-rich domain mediates peptide sensing in FLS2–flg22–BAK1 complex[J]. Chin. Phys. B, 2020, 29(10): 108709-.

图/表 8

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Twisting mode of supercoil leucine-rich domain mediates peptide sensing in FLS2–flg22–BAK1 complex

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