Improved data analysis method of single-molecule experiments based on probability optimization

doi:10.1088/1674-1056/27/1/018703

中国物理B ›› 2018, Vol. 27 ›› Issue (1): 18703-018703.doi: 10.1088/1674-1056/27/1/018703

所属专题： SPECIAL TOPIC — Soft matter and biological physics

• SPECIAL TOPIC—Soft matter and biological physics • 上一篇下一篇

Improved data analysis method of single-molecule experiments based on probability optimization

Weili Zhai(翟伟利), Guohua Yuan(袁国华), Chao Liu(刘超), Hu Chen(陈虎)

Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, Department of Physics, Xiamen University, Xiamen 361005, China

收稿日期:2017-10-09 修回日期:2017-10-20 出版日期:2018-01-05 发布日期:2018-01-05
通讯作者: Hu Chen E-mail:chenhu@xmu.edu.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant Nos. 11474237 and 11574310) and the 111 Project, China (Grant No. B16029).

Improved data analysis method of single-molecule experiments based on probability optimization

Weili Zhai(翟伟利), Guohua Yuan(袁国华), Chao Liu(刘超), Hu Chen(陈虎)

Research Institute for Biomimetics and Soft Matter, Fujian Provincial Key Laboratory for Soft Functional Materials Research, Department of Physics, Xiamen University, Xiamen 361005, China

Received:2017-10-09 Revised:2017-10-20 Online:2018-01-05 Published:2018-01-05
Contact: Hu Chen E-mail:chenhu@xmu.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant Nos. 11474237 and 11574310) and the 111 Project, China (Grant No. B16029).

摘要/Abstract

摘要： To extract the dynamic parameters from single molecule manipulation experiments, usually lots of data at different forces need to be recorded. But the measuring time of a single molecule is limited due to breakage of the tether or degradation of the molecule. Here we propose a data analysis method based on probability maximization of the recorded time trace to extract the dynamic parameters from a single measurement. The feasibility of this method was verified by dealing with the simulation data of a two-state system. We also applied this method to estimate the parameters of DNA hairpin folding and unfolding dynamics measured by a magnetic tweezers experiment.

关键词: probability optimization, Bell's model, DNA hairpin, single molecule manipulation

Abstract: To extract the dynamic parameters from single molecule manipulation experiments, usually lots of data at different forces need to be recorded. But the measuring time of a single molecule is limited due to breakage of the tether or degradation of the molecule. Here we propose a data analysis method based on probability maximization of the recorded time trace to extract the dynamic parameters from a single measurement. The feasibility of this method was verified by dealing with the simulation data of a two-state system. We also applied this method to estimate the parameters of DNA hairpin folding and unfolding dynamics measured by a magnetic tweezers experiment.

Key words: probability optimization, Bell's model, DNA hairpin, single molecule manipulation

中图分类号: (Single-molecule techniques)

87.80.Nj

82.37.Rs (Single molecule manipulation of proteins and other biological molecules) 87.15.A- (Theory, modeling, and computer simulation) 02.50.-r (Probability theory, stochastic processes, and statistics)

翟伟利, 袁国华, 刘超, 陈虎. Improved data analysis method of single-molecule experiments based on probability optimization[J]. 中国物理B, 2018, 27(1): 18703-018703.

Weili Zhai(翟伟利), Guohua Yuan(袁国华), Chao Liu(刘超), Hu Chen(陈虎). Improved data analysis method of single-molecule experiments based on probability optimization[J]. Chin. Phys. B, 2018, 27(1): 18703-018703.

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Improved data analysis method of single-molecule experiments based on probability optimization

Improved data analysis method of single-molecule experiments based on probability optimization

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