中国物理B ›› 2017, Vol. 26 ›› Issue (12): 128708-128708.doi: 10.1088/1674-1056/26/12/128708
所属专题： SPECIAL TOPIC — Soft matter and biological physics
• SPECIAL TOPIC—Soft matter and biological physics • 上一篇 下一篇
Protein-membrane interactions investigated with surface-induced fluorescence attenuation
Li Ma(马丽), Ying Li(李颖), Ming Li(李明), Shuxin Hu(胡书新)
- 1. Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China
Protein-membrane interactions investigated with surface-induced fluorescence attenuation
Li Ma(马丽)1,2, Ying Li(李颖)1, Ming Li(李明)1, Shuxin Hu(胡书新)1
- 1. Key Laboratory of Soft Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China
摘要：
Research on protein-membrane interactions has been undeveloped due to the lack of proper techniques to detect the position of proteins at membranes because membranes are usually only about 4-nm thick. We have recently developed a new method named surface-induced fluorescence attenuation (SIFA) to track both vertical and lateral kinetics of a single labelling dye in supported lipid bilayers. It takes advantage of strong interaction between a light-emitting dye and a partially reflecting surface. By applying the technique to membrane proteins being fluorescently labelled at different residues, here we show that SIFA can measure not only the insertion depth of a dye inside a lipid bilayer, but also the position of a dye in solution near the surface. SIFA can therefore be used to study membrane proteins of various types.
中图分类号: (Protein-membrane interactions)
- 87.15.kt