›› 2014, Vol. 23 ›› Issue (10): 104202-104202.doi: 10.1088/1674-1056/23/10/104202

• ELECTROMAGNETISM, OPTICS, ACOUSTICS, HEAT TRANSFER, CLASSICAL MECHANICS, AND FLUID DYNAMICS • 上一篇    下一篇

Analysis of detection limit to time-resolved coherent anti-Stokes Raman scattering nanoscopy

刘伟, 刘双龙, 陈丹妮, 牛憨笨   

  1. Institute of Optoelectronics, Key Laboratory of Optoelectronic Deviced and Systems of Education Ministry, Shenzhen University, Shenzhen 518060, China
  • 收稿日期:2013-12-26 修回日期:2014-03-14 出版日期:2014-10-15 发布日期:2014-10-15
  • 基金资助:
    Project supported by the National Basic Research Program of China (Grant No. 2012CB825802), the Major Scientific Instruments Equipment Development of China (Grant No. 2012YQ15009203), the National Natural Science Foundation of China (Grant Nos. 60878053 and 11004136), and the State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, China (Grant No. DL12-01).

Analysis of detection limit to time-resolved coherent anti-Stokes Raman scattering nanoscopy

Liu Wei (刘伟), Liu Shuang-Long (刘双龙), Chen Dan-Ni (陈丹妮), Niu Han-Ben (牛憨笨)   

  1. Institute of Optoelectronics, Key Laboratory of Optoelectronic Deviced and Systems of Education Ministry, Shenzhen University, Shenzhen 518060, China
  • Received:2013-12-26 Revised:2014-03-14 Online:2014-10-15 Published:2014-10-15
  • Contact: Chen Dan-Ni,Niu Han-Ben E-mail:dannyc007@163.com;hbniu@szu.edu.cn
  • About author:42.25.Fx; 42.65.Dr; 42.65.-k; 87.64.-t
  • Supported by:
    Project supported by the National Basic Research Program of China (Grant No. 2012CB825802), the Major Scientific Instruments Equipment Development of China (Grant No. 2012YQ15009203), the National Natural Science Foundation of China (Grant Nos. 60878053 and 11004136), and the State Key Laboratory of Precision Measurement Technology and Instruments, Tsinghua University, China (Grant No. DL12-01).

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摘要： In the implementation of CARS nanoscopy, signal strength decreases with focal volume size decreasing. A crucial problem that remains to be solved is whether the reduced signal generated in the suppressed focal volume can be detected. Here reported is a theoretical analysis of detection limit (DL) to time-resolved CARS (T-CARS) nanoscopy based on our proposed additional probe-beam-induced phonon depletion (APIPD) method for the low concentration samples. In order to acquire a detailed shot-noise limited signal-to-noise (SNR) and the involved parameters to evaluate DL, the T-CARS process is described with full quantum theory to estimate the extreme power density levels of the pump and Stokes beams determined by saturation behavior of coherent phonons, which are both actually on the order of ～ 1019 W/cm2. When the pump and Stokes intensities reach such values and the total intensity of the excitation beams arrives at a maximum tolerable by most biological samples in a certain suppressed focal volume (40-nm suppressed focal scale in APIPD method), the DL correspondingly varies with exposure time, for example, DL values are 103 and 102 when exposure times are 20 ms and 200 ms respectively.

关键词: break through the diffraction limit, coherent anti-Stokes Raman scattering, nonlinear optics, detection limit

Abstract: In the implementation of CARS nanoscopy, signal strength decreases with focal volume size decreasing. A crucial problem that remains to be solved is whether the reduced signal generated in the suppressed focal volume can be detected. Here reported is a theoretical analysis of detection limit (DL) to time-resolved CARS (T-CARS) nanoscopy based on our proposed additional probe-beam-induced phonon depletion (APIPD) method for the low concentration samples. In order to acquire a detailed shot-noise limited signal-to-noise (SNR) and the involved parameters to evaluate DL, the T-CARS process is described with full quantum theory to estimate the extreme power density levels of the pump and Stokes beams determined by saturation behavior of coherent phonons, which are both actually on the order of ～ 1019 W/cm2. When the pump and Stokes intensities reach such values and the total intensity of the excitation beams arrives at a maximum tolerable by most biological samples in a certain suppressed focal volume (40-nm suppressed focal scale in APIPD method), the DL correspondingly varies with exposure time, for example, DL values are 103 and 102 when exposure times are 20 ms and 200 ms respectively.

Key words: break through the diffraction limit, coherent anti-Stokes Raman scattering, nonlinear optics, detection limit

中图分类号:  (Diffraction and scattering)

  • 42.25.Fx
42.65.Dr (Stimulated Raman scattering; CARS) 42.65.-k (Nonlinear optics) 87.64.-t (Spectroscopic and microscopic techniques in biophysics and medical physics)