中国物理B ›› 2011, Vol. 20 ›› Issue (2): 24301-024301.doi: 10.1088/1674-1056/20/2/024301

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Correlation between microbubble-induced acoustic cavitation and hemolysis in vitro

郭霞生1, 章东1, 张春兵2, 刘政3   

  1. (1)Institute of Acoustics, Key Laboratory of Modern Acoustics (Nanjing Universit25), Ministry of Education, Nanjing University, Nanjing 210093, China; (2)The Traditional Chinese Medicine Hospital of Jiangsu Province, Nanjing 210029, China; Institute of Acoustics, Key Laboratory of Modern Acoustics (Nanjing Universit25), Ministry of Education, Nanjing University, Nanjing 210093, China; (3)Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
  • 收稿日期:2010-06-17 修回日期:2010-07-12 出版日期:2011-02-15 发布日期:2011-02-15
  • 基金资助:
    Project supported by the National Basic Research Program of China (Grant No. 2010CB732600), the National Natural Science Foundation of China (Grant Nos. 10774071, 10974093, 10974098, and 30672014), the Natural Science Foundation of Jiangsu Province of China (Grant NO. BE2010768), and the Fund of the State Key Lab of Acoustics.

Correlation between microbubble-induced acoustic cavitation and hemolysis in vitro

Zhang Chun-Bing(张春兵)a)b),Liu Zheng(刘政)c), Guo Xia-sheng(郭霞生)b),and Zhang Dong(章东)b)†   

  1. a The Traditional Chinese Medicine Hospital of Jiangsu Province, Nanjing 210029, China; b Institute of Acoustics, Key Laboratory of Modern Acoustics (Nanjing University), Ministry of Education, Nanjing University, Nanjing 210093, China; c Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China
  • Received:2010-06-17 Revised:2010-07-12 Online:2011-02-15 Published:2011-02-15
  • Supported by:
    Project supported by the National Basic Research Program of China (Grant No. 2010CB732600), the National Natural Science Foundation of China (Grant Nos. 10774071, 10974093, 10974098, and 30672014), the Natural Science Foundation of Jiangsu Province of China (Grant NO. BE2010768), and the Fund of the State Key Lab of Acoustics.

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摘要： Microbubbles promise to enhance the efficiency of ultrasound-mediated drug delivery and gene therapy by taking advantage of artificial cavitation nuclei. The purpose of this study is to examine the ultrasound-induced hemolysis in the application of drug delivery in the presence of microbubbles. To achieve this goal, human red blood cells mixed with microbubbles were exposed to 1-MHz pulsed ultrasound. The hemolysis level was measured by a flow cytometry, and the cavitation dose was detected by a passive cavitation detecting system. The results demonstrate that larger cavitation dose would be generated with the increase of acoustic pressure, which might give rise to the enhancement of hemolysis. Besides the experimental observations, the acoustic pressure dependence of the radial oscillation of microbubble was theoretically estimated. The comparison between the experimental and calculation results indicates that the hemolysis should be highly correlated to the acoustic cavitation.

关键词: acoustic cavitation, passive cavitation detection, microbubbles, hemolysis

Abstract: Microbubbles promise to enhance the efficiency of ultrasound-mediated drug delivery and gene therapy by taking advantage of artificial cavitation nuclei. The purpose of this study is to examine the ultrasound-induced hemolysis in the application of drug delivery in the presence of microbubbles. To achieve this goal, human red blood cells mixed with microbubbles were exposed to 1-MHz pulsed ultrasound. The hemolysis level was measured by a flow cytometry, and the cavitation dose was detected by a passive cavitation detecting system. The results demonstrate that larger cavitation dose would be generated with the increase of acoustic pressure, which might give rise to the enhancement of hemolysis. Besides the experimental observations, the acoustic pressure dependence of the radial oscillation of microbubble was theoretically estimated. The comparison between the experimental and calculation results indicates that the hemolysis should be highly correlated to the acoustic cavitation.

Key words: acoustic cavitation, passive cavitation detection, microbubbles, hemolysis

中图分类号:  (Nonlinear acoustics)

  • 43.25.+y
43.35.+d (Ultrasonics, quantum acoustics, and physical effects of sound) 43.80.+p (Bioacoustics)