中国物理B ›› 2005, Vol. 14 ›› Issue (5): 935-941.doi: 10.1088/1009-1963/14/5/013

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Imaging properties of coherent anti-Stokes Raman scattering microscope　　　　　

肖繁荣1, 王桂英1, 徐至展1, 袁景和2   

  1. (1)Key Laboratory for High Intensity Optics, the Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China.; (2)Key Laboratory for High Intensity Optics, the Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China;Institute of Science and technology for Opto-electron information,Yantai University, Yantai 264005, China.
  • 收稿日期:2004-05-14 修回日期:2005-01-21 出版日期:2005-05-19 发布日期:2005-05-19

Imaging properties of coherent anti-Stokes Raman scattering microscope

Yuan Jing-He (袁景和)ab, Xiao Fan-Rong (肖繁荣)a, Wang Gui-Ying (王桂英)a, Xu Zhi-Zhan (徐至展)a   

  1. a Key Laboratory for High Intensity Optics, the Shanghai Institute of Optics and Fine Mechanics, Chinese Academy of Sciences, Shanghai 201800, China.; b Institute of Science and technology for Opto-electron information,Yantai University, Yantai 264005, China.
  • Received:2004-05-14 Revised:2005-01-21 Online:2005-05-19 Published:2005-05-19

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摘要： Coherent anti-Stokes Raman scattering (CARS) microscope with the combination of confocal and CARS techniques is a remarkable alternative for imaging chemical or biological specimens that neither fluoresce nor tolerate labeling. CARS is a nonlinear optical process, the imaging properties of CARS microscopy will be very different from the conventional confocal microscope. In this paper, the intensity distribution and the polarization property of the optical field near the focus was calculated. By using the Green function, the precise analytic solution to the wave equation of a Hertzian dipole source was obtained. We found that the intensity distributions vary considerably with the different experimental configurations and the different specimen shapes. So the conventional description of microscope (e.g. the point spread function) will fail to describe the imaging properties of CARS microscope.

关键词: microscope, coherent anti-Stokes Raman scattering, Hertzian dipole, Green function.

Abstract: Coherent anti-Stokes Raman scattering (CARS) microscope with the combination of confocal and CARS techniques is a remarkable alternative for imaging chemical or biological specimens that neither fluoresce nor tolerate labeling. CARS is a nonlinear optical process, the imaging properties of CARS microscopy will be very different from the conventional confocal microscope. In this paper, the intensity distribution and the polarization property of the optical field near the focus was calculated. By using the Green function, the precise analytic solution to the wave equation of a Hertzian dipole source was obtained. We found that the intensity distributions vary considerably with the different experimental configurations and the different specimen shapes. So the conventional description of microscope (e.g. the point spread function) will fail to describe the imaging properties of CARS microscope.

Key words: microscope, coherent anti-Stokes Raman scattering, Hertzian dipole, Green function.

中图分类号: 

  • 0760P.