中国物理B ›› 2021, Vol. 30 ›› Issue (5): 54203-054203.doi: 10.1088/1674-1056/abda32

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A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment

Kun-Yang Wang(王坤阳)1,2,3, Jie Shao(邵杰)3,†, Li-Gang Shao(邵李刚)1, Jia-Jin Chen(陈家金)1, Gui-Shi Wang(王贵师)1, Kun Liu(刘琨)1, and Xiao-Ming Gao(高晓明)1,2   

  1. 1 Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Science, Hefei 230031, China;
    2 University of Science and Technology of China, Hefei 230031, China;
    3 College of Physics and Electronic Information Engineering, Zhejiang Normal University, Zhejiang 321004, China
  • 收稿日期:2020-12-20 修回日期:2021-01-07 接受日期:2021-01-11 出版日期:2021-05-14 发布日期:2021-05-14
  • 通讯作者: Jie Shao E-mail:shaojie@zjnu.cn
  • 基金资助:
    Project supported by the National Key Research and Development Program of China (Grant Nos. 2017YFC0209700 and 2016YFC0303900) and the National Natural Science Foundation of China (Grant No. 41730103).

A pressure-calibration method of wavelength modulation spectroscopy in sealed microbial growth environment

Kun-Yang Wang(王坤阳)1,2,3, Jie Shao(邵杰)3,†, Li-Gang Shao(邵李刚)1, Jia-Jin Chen(陈家金)1, Gui-Shi Wang(王贵师)1, Kun Liu(刘琨)1, and Xiao-Ming Gao(高晓明)1,2   

  1. 1 Anhui Institute of Optics and Fine Mechanics, Chinese Academy of Science, Hefei 230031, China;
    2 University of Science and Technology of China, Hefei 230031, China;
    3 College of Physics and Electronic Information Engineering, Zhejiang Normal University, Zhejiang 321004, China
  • Received:2020-12-20 Revised:2021-01-07 Accepted:2021-01-11 Online:2021-05-14 Published:2021-05-14
  • Contact: Jie Shao E-mail:shaojie@zjnu.cn
  • Supported by:
    Project supported by the National Key Research and Development Program of China (Grant Nos. 2017YFC0209700 and 2016YFC0303900) and the National Natural Science Foundation of China (Grant No. 41730103).

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摘要： A new pressure-calibration method for calibrating the reduction of second harmonic (2f) amplitude caused by pressure broadening effect in sealed microbial growth environment is present. The new method combines with linewidth compensation and modulation depth compensation and makes the 2f amplitude accurately retrieve metabolic CO2 in microbial growth. In order to verify the method, a simulation experiment is developed, in which the increasing CO2 concentration leads to the increasing pressure. Comparing with the relation between the traditional 2f amplitude and gas concentration, there is a monotonous relation between the calibrated 2f amplitude and CO2 concentration, particularly, a linear relation is present when the CO2 concentration is replaced with the CO2 particle number. In terms of microbial measurement, the growth of Escherichia coli is measured, and the culture bottle is sealed during the microbial growth process. The experimental results show that, comparing to the microbial growth retrieved by traditional 2f amplitude, the calibrated 2f amplitude can accurately retrieve microbial growth in sealed environment.

关键词: second harmonic amplitude, pressure-calibration, microbial growth, sealed environment, CO2

Abstract: A new pressure-calibration method for calibrating the reduction of second harmonic (2f) amplitude caused by pressure broadening effect in sealed microbial growth environment is present. The new method combines with linewidth compensation and modulation depth compensation and makes the 2f amplitude accurately retrieve metabolic CO2 in microbial growth. In order to verify the method, a simulation experiment is developed, in which the increasing CO2 concentration leads to the increasing pressure. Comparing with the relation between the traditional 2f amplitude and gas concentration, there is a monotonous relation between the calibrated 2f amplitude and CO2 concentration, particularly, a linear relation is present when the CO2 concentration is replaced with the CO2 particle number. In terms of microbial measurement, the growth of Escherichia coli is measured, and the culture bottle is sealed during the microbial growth process. The experimental results show that, comparing to the microbial growth retrieved by traditional 2f amplitude, the calibrated 2f amplitude can accurately retrieve microbial growth in sealed environment.

Key words: second harmonic amplitude, pressure-calibration, microbial growth, sealed environment, CO2

中图分类号:  (Semiconductor lasers; laser diodes)

  • 42.55.Px
42.62.Fi (Laser spectroscopy) 33.20.Ea (Infrared spectra)