Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

doi:10.1088/1674-1056/25/7/078201

中国物理B ›› 2016, Vol. 25 ›› Issue (7): 78201-078201.doi: 10.1088/1674-1056/25/7/078201

• INTERDISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY • 上一篇下一篇

Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

Fengyun Yang(杨凤云), Kaige Wang(王凯歌), Dan Sun(孙聃), Wei Zhao(赵伟), Hai-qing Wang(王海青), Xin He(何鑫), Gui-ren Wang(王归仁), Jin-tao Bai(白晋涛)

1 Institute of Photonics & Photo-Technology, National Center for International Research of Photoelectric Technology & Nano-functional Materials, State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Key Laboratory of Optoelectronic Technology of Shaanxi Province, Northwest University, Xi’an 710069, China;
2 Mechanical Engineering Department & Biomedical Engineering Program, University of South Carolina, Columbia SC 29208, USA

收稿日期:2016-02-02 修回日期:2016-03-22 出版日期:2016-07-05 发布日期:2016-07-05
通讯作者: Kaige Wang E-mail:wangkg@nwu.edu.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant No. 61378083), the International Cooperation Foundation of the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant No. 2011DFA12220), the Major Research Plan of National Natural Science Foundation of China (Grant No. 91123030), and the Natural Science Foundation of Shaanxi Province of China (Grant Nos. 2010JS110 and 2013SZS03-Z01).

Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

Fengyun Yang(杨凤云)¹, Kaige Wang(王凯歌)¹, Dan Sun(孙聃)¹, Wei Zhao(赵伟)^1,2, Hai-qing Wang(王海青)¹, Xin He(何鑫)¹, Gui-ren Wang(王归仁)², Jin-tao Bai(白晋涛)¹

1 Institute of Photonics & Photo-Technology, National Center for International Research of Photoelectric Technology & Nano-functional Materials, State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Key Laboratory of Optoelectronic Technology of Shaanxi Province, Northwest University, Xi’an 710069, China;
2 Mechanical Engineering Department & Biomedical Engineering Program, University of South Carolina, Columbia SC 29208, USA

Received:2016-02-02 Revised:2016-03-22 Online:2016-07-05 Published:2016-07-05
Contact: Kaige Wang E-mail:wangkg@nwu.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant No. 61378083), the International Cooperation Foundation of the National Science and Technology Major Project of the Ministry of Science and Technology of China (Grant No. 2011DFA12220), the Major Research Plan of National Natural Science Foundation of China (Grant No. 91123030), and the Natural Science Foundation of Shaanxi Province of China (Grant Nos. 2010JS110 and 2013SZS03-Z01).

摘要/Abstract

摘要：

The electrodynamic characteristics of single DNA molecules moving within micro-/nano-fluidic channels are important in the design of biomedical chips and bimolecular sensors. In this study, the dynamic properties of λ -DNA molecules transferring along the microchannels driven by the external electrickinetic force were systemically investigated with the single molecule fluorescence imaging technique. The experimental results indicated that the velocity of DNA molecules was strictly dependent on the value of the applied electric field and the diameter of the channel. The larger the external electric field, the larger the velocity, and the more significant deformation of DNA molecules. More meaningfully, it was found that the moving directions of DNA molecules had two completely different directions: (i) along the direction of the external electric field, when the electric field intensity was smaller than a certain threshold value; (ii) opposite to the direction of the external electric field, when the electric field intensity was greater than the threshold electric field intensity. The reversal movement of DNA molecules was mainly determined by the competition between the electrophoresis force and the influence of electro-osmosis flow. These new findings will theoretically guide the practical application of fluidic channel sensors and lab-on-chips for precisely manipulating single DNA molecules.

关键词: reversal movement, electrophoresis, electroosmosis, electric field threshold value

Abstract:

Key words: reversal movement, electrophoresis, electroosmosis, electric field threshold value

中图分类号: (Single molecule manipulation of proteins and other biological molecules)

82.37.Rs

87.15.H- (Dynamics of biomolecules) 87.15.Tt (Electrophoresis) 87.19.rh (Fluid transport and rheology)

杨凤云, 王凯歌, 孙聃, 赵伟, 王海青, 何鑫, 王归仁, 白晋涛. Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique[J]. 中国物理B, 2016, 25(7): 78201-078201.

Fengyun Yang(杨凤云), Kaige Wang(王凯歌), Dan Sun(孙聃), Wei Zhao(赵伟), Hai-qing Wang(王海青), Xin He(何鑫), Gui-ren Wang(王归仁), Jin-tao Bai(白晋涛). Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique[J]. Chin. Phys. B, 2016, 25(7): 78201-078201.

参考文献 63

[1]	Ha T and Tinnefeld P 2012 Ann. Rev. Phys. Chem. 63 595
[2]	Mai D J, Brockman C and Schroeder C M 2012 Soft Matter 8 10560
[3]	Yu J, Xiao J, Ren X J, Lao K Q and Xie X S 2006 Science 311 1600
[4]	Cai L, Friedman N and Xie X S 2006 Nature 440 358
[5]	Mivelle M, Zanten T, Manzo C and Garcia-Parajo M F 2014 Microsc. Res. Tech. 77 537
[6]	Renner C B and Doyle P S 2015 Soft Matter 11 3105
[7]	Whitesides G M 2006 Nature 442 368
[8]	Mairhofer J, Roppert K and Ertl P 2009 Sensors 9 4804
[9]	Liu C, Qu Y Y, Luo Y and Fang N 2011 Electrophoresis 32 3308
[10]	Gupta C, Liao W C, Gallego-Perez D, Castro C and Lee L 2014 Biomicrofluidics 8 024114
[11]	Tegenfeldt J O, Prinz C, Cao H, Huang R L, Austin R H, Chou S Y, Cox E C and Sturm J C 2004 Anal. Bioanal. Chem. 378 1678
[12]	Min S K, Kim W Y, Cho Y and Kim K S 2011 Nat. Nanotechnol. 6 162
[13]	Mauk M G, Liu C, Sadik M and Bau H H 2015 Mobile Health Technologies: Methods and Protocols 1256 15
[14]	Firnkes M, Pedone D, Knezevic J, Doblinger M and Rant U 2010 Nano Lett. 10 2162
[15]	Tang J and Doyle P S 2007 Appl. Phys. Lett. 90 224103
[16]	Yeh J W, Taloni A, Chen Y L and Chou C F 2012 Nano Lett. 12 1597
[17]	Balducci A, Mao P, Han J and Doyle P S 2006 Macromolecules 39 6273
[18]	Yeo L Y, Chang H C, Chan P P and Friend J R 2011 Small 7 12
[19]	Santhiago M, Nery E W, Santos G P and Kubota L T 2014 Bioanalysis 6 89
[20]	Tang J 2010 Single Molecule DNA Dynamics in Micro-and Nano-fluidic Devices. Massachusetts Institute of Technology p.140
[21]	Chang H C and Yeo L Y 2010 Electrokinetically Driven Microfluidics and Nanofluidics (Cambridge : Cambridge University Press) p. 39
[22]	Zhang B, Wood M and Lee H 2009 Anal. Chem. 81 5541
[23]	Meller A and Branton D 2002 Electrophoresis 23 2583
[24]	Schoch R B, Han J and Renaud P 2008 Rev. Mod. Phys. 80 839
[25]	Liu Q J, Wu H W, Wu L Z, Xie X, Kong J L, Ye X F and Liu L P 2012 PLoS One 7 e46014
[26]	Bhattacharyya S and Gopmandal P P 2013 Soft Matter 9 1871
[27]	Tatsumi K, Nishitani K, Fukuda K, Katsumoto Y and Nakabe K 2010 Meas. Sci. Technol. 21 105402
[28]	Bown M, MacInnes J, Allen R and Zimmerman W 2006 Meas. Sci. Technol. 17 2175
[29]	Samouda F, Colin S, Barrot C, Baldas L and Brandner J J 2015 Microsyst. Technol. 21 527
[30]	Gerashchenko S and Steinberg V 2006 Phys. Rev. Lett. 96 038304
[31]	Teixeira R E, Babcock H P, Shaqfeh E S and Chu S 2005 Macromolecules 38 581
[32]	Hanasaki I, Yukimoto N, Uehara S, Shintaku H and Kawano S 2015 J. Phys. D: Appl. Phys. 48 135402
[33]	Manneschi C, Fanzio P, Ala-Nissila T, Angeli E, Repetto L, Firpo G and Valbusa U 2014 Biomicrofluidics 8 064121
[34]	Glasgow I, Batton J and Aubry N 2004 Lab on a Chip 4 558
[35]	Shaqfeh E S 2005 J. Non-Newtonian Fluid Mech. 130 1
[36]	Laib S, Robertson R M and Smith D E 2006 Macromolecules 39 4115
[37]	Lim Y, Kouzani A and Duan W 2010 Microsystem Technologies 16 1995
[38]	van Kan J A, Zhang C, Malar P P and van der Maarel J R 2012 Biomicrofluidics 6 036502
[39]	Xu W L and Muller S J 2012 Lab on a Chip 12 647
[40]	Perkins T T, Smith D and Chu S 1994 Science 264 822
[41]	Perkins T T, Smith D E and Chu S 1997 Science 276 2016
[42]	Smith D E, Babcock H P and Chu S 1999 Science 283 1724
[43]	Duan C H, Wang W and Xie Q 2013 Biomicrofluidics 7 026501
[44]	Kang G, No K and Kim G 2006 Int. J. Mod. Phys. B 20 4493
[45]	Becker H and Gärtner C 2008 Anal. Bioanal. Chem. 390 89
[46]	Yang Y Y, Wang K G, Qiao Q W, Li D, Wang S, Ren Z Y and Bai J 2012 12th IEEE Conference on Nanotechnology (IEEE-NANO) p. 20
[47]	Gao Z Y, Wang K G, Zhang C, Ma H W, Wang G R and Bai J T 2014 Sci. China Technol. Sci. 57 249
[48]	Ma H W, Wang K G, Gao Z Y, Wang H Q, Wang S, Zhang C, Wang G and Bai J 2014 AIP Adv. 4 107139
[49]	Polcari A, Romano P, Sabatino L, Del Vecchio E, Consales M, Cusano A, Cutolo A and Colantuoni V 2011 J. Appl. Phys. 109 074703
[50]	Tang J, Du N and Doyle P S 2011 Proceedings of the National Academy of Sciences 108 16153
[51]	Vasdekis A E, Ońeil C P, Hubbell J A and Psaltis D 2010 Biomacromolecules 11 827
[52]	Marie R, Beech J P, Vörös J, Tegenfeldt J O and Höök F 2006 Langmuir 22 10103
[53]	Liu L and Zhu L Z 2015 Nanoscale Res. Lett. 10 1
[54]	Ren Y K, Yan H, Jiang H Y, Gu J Z and Antonio R 2009 Chin. Phys. B 18 4349
[55]	Wei Q Q, Wei Z J, Ren L M, Zhao H B, Ye T Y, Shi Z J, Fu Y Y, Zhang X and Huang R 2012 Chin. Phys. B 21 088103
[56]	http://enwikipediaorg/wiki/Dielectrophoresis
[57]	Ren Y, Wu H, Feng G, Hou L and Jiang H 2014 Chin. J. Mech. Eng. 27 103
[58]	Smoluchowski M 1918 Naturwissenschaften 6 253
[59]	Hunter R J 1982 J. Colloid Interface Sci. 88 608
[60]	Mannion J, Reccius C, Cross J and Craighead H 2006 Biophys. J. 90 4538
[61]	Han J and Craighead H G 2002 Anal. Chem. 74 394
[62]	Han J, Turner S and Craighead H G 1999 Phys. Rev. Lett. 83 1688
[63]	Yuan Q Z and Zhao Y P 2009 J. Am. Chem. Soc. 131 6374

Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

Direct observation of λ -DNA molecule reversal movement within microfluidic channels under electric field with single molecule imaging technique

RichHTML

PDF (PC)

赞

可视化

摘要/Abstract

引用本文

使用本文

参考文献 63

相关文章 7

Metrics

本文评价

推荐阅读 0

[1]	Bing Yan(严兵), Bo Chen(陈波), Zerui Peng(彭泽瑞), and Yong-Liang Xiong(熊永亮). Particle captured by a field-modulating vortex through dielectrophoresis force[J]. 中国物理B, 2022, 31(3): 34703-034703.
[2]	Bing Yan(严兵), Bo Chen(陈波), Yongliang Xiong(熊永亮), and Zerui Peng(彭泽瑞). Effect of Joule heating on the electroosmotic microvortex and dielectrophoretic particle separation controlled by local electric field[J]. 中国物理B, 2021, 30(11): 114701-114701.
[3]	廖麦嘉, 魏名佐, 徐士鑫, 歐陽新喬, 沈平. Non-Stokes drag coefficient in single-particle electrophoresis:New insights on a classical problem[J]. 中国物理B, 2019, 28(8): 84701-084701.
[4]	王志强, 颜志波, 秦明辉, 高兴森, 刘俊明. Dynamic resistive switching in a three-terminal device based on phase separated manganites[J]. , 2015, 24(3): 37101-037101.
[5]	魏芹芹, 魏子钧, 任黎明, 赵华波, 叶天扬, 施祖进, 傅云义, 张兴, 黄如. Vertical assembly of carbon nanotubes for via interconnects[J]. 中国物理B, 2012, 21(8): 88103-088103.
[6]	姜洪源, 任玉坤, 陶冶. Microwire formation based on dielectrophoresis of electroless gold plated polystyrene microspheres[J]. 中国物理B, 2011, 20(5): 57701-057701.
[7]	林建忠, 张凯, 李惠君. Study on the mixing of fluid in curved microchannelswith heterogeneous surface potentials[J]. 中国物理B, 2006, 15(11): 2688-2696.