Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers

doi:10.1088/1674-1056/27/6/068701

中国物理B ›› 2018, Vol. 27 ›› Issue (6): 68701-068701.doi: 10.1088/1674-1056/27/6/068701

• INTERDISCIPLINARY PHYSICS AND RELATED AREAS OF SCIENCE AND TECHNOLOGY • 上一篇下一篇

Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers

Yi-Zhou Wang(王一舟), Xi-Miao Hou(侯锡苗), Hai-Peng Ju(车海鹏), Xue Xiao(肖雪), Xu-Guang Xi(奚绪光), Shuo-Xing Dou(窦硕星), Peng-Ye Wang(王鹏业), Wei Li(李伟)

1 Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
2 School of Life Sciences, Northwest A & F University, Yangling 712100, China;
3 School of Physical Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

收稿日期:2017-12-25 修回日期:2018-03-21 出版日期:2018-06-05 发布日期:2018-06-05
通讯作者: Peng-Ye Wang, Wei Li E-mail:pywang@iphy.ac.cn;weili007@iphy.ac.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant Nos.11474346 and 11774407),the Key Research Program of Frontier Sciences,Chinese Academy of Sciences (Grant No.QYZDB-SSW-SLH045),and the National Key Research and Development Program,China (Grant No.2016YFA0301500).

Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers

Yi-Zhou Wang(王一舟)^1,3, Xi-Miao Hou(侯锡苗)², Hai-Peng Ju(车海鹏)¹, Xue Xiao(肖雪)^1,3, Xu-Guang Xi(奚绪光)², Shuo-Xing Dou(窦硕星)^1,3, Peng-Ye Wang(王鹏业)^1,3, Wei Li(李伟)¹

1 Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China;
2 School of Life Sciences, Northwest A & F University, Yangling 712100, China;
3 School of Physical Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

Received:2017-12-25 Revised:2018-03-21 Online:2018-06-05 Published:2018-06-05
Contact: Peng-Ye Wang, Wei Li E-mail:pywang@iphy.ac.cn;weili007@iphy.ac.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant Nos.11474346 and 11774407),the Key Research Program of Frontier Sciences,Chinese Academy of Sciences (Grant No.QYZDB-SSW-SLH045),and the National Key Research and Development Program,China (Grant No.2016YFA0301500).

摘要/Abstract

摘要： Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work, we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K⁺ buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.

关键词: biophysics, G-quadruplexes, single-molecule studies, DNA structures

Abstract: Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work, we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K⁺ buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.

Key words: biophysics, G-quadruplexes, single-molecule studies, DNA structures

中图分类号: (Single-molecule techniques)

87.80.Nj

87.14.gk (DNA) 87.15.H- (Dynamics of biomolecules)

王一舟, 侯锡苗, 车海鹏, 肖雪, 奚绪光, 窦硕星, 王鹏业, 李伟. Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers[J]. 中国物理B, 2018, 27(6): 68701-068701.

Yi-Zhou Wang(王一舟), Xi-Miao Hou(侯锡苗), Hai-Peng Ju(车海鹏), Xue Xiao(肖雪), Xu-Guang Xi(奚绪光), Shuo-Xing Dou(窦硕星), Peng-Ye Wang(王鹏业), Wei Li(李伟). Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers[J]. Chin. Phys. B, 2018, 27(6): 68701-068701.

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Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers

Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers

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