The engineering of doxorubicin-loaded liposome-quantum dot hybrids for cancer theranostics

doi:10.1088/1674-1056/23/8/087805

中国物理B ›› 2014, Vol. 23 ›› Issue (8): 87805-087805.doi: 10.1088/1674-1056/23/8/087805

• SPECIAL TOPI—International Conference on Nanoscience & Technology, China 2013 • 上一篇下一篇

The engineering of doxorubicin-loaded liposome-quantum dot hybrids for cancer theranostics

田博文^{a b}, Wafa' T. Al-Jamal^{a c}, Kostas Kostarelos^{a d}

a Nanomedicine Laboratory, UCL School of Pharmacy, University College London, London WC1N 1AX, UK;
b Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, UK;
c School of Pharmacy, University of East Anglia, Norwich, UK;
d Nanomedicine Laboratory, Faculty of Medical & Human Sciences, University of Manchester, UK

收稿日期:2013-09-04 修回日期:2014-04-16 出版日期:2014-08-15 发布日期:2014-08-15
基金资助:
Project partially supported by the School of Pharmacy, University College London.

The engineering of doxorubicin-loaded liposome-quantum dot hybrids for cancer theranostics

Bowen Tian (田博文)^{a b}, Wafa' T. Al-Jamal^{a c}, Kostas Kostarelos^{a d}

a Nanomedicine Laboratory, UCL School of Pharmacy, University College London, London WC1N 1AX, UK;
b Laboratory of Biophysics and Surface Analysis, School of Pharmacy, University of Nottingham, Nottingham, UK;
c School of Pharmacy, University of East Anglia, Norwich, UK;
d Nanomedicine Laboratory, Faculty of Medical & Human Sciences, University of Manchester, UK

Received:2013-09-04 Revised:2014-04-16 Online:2014-08-15 Published:2014-08-15
Contact: Bowen Tian E-mail:bowen.tian@nottingham.ac.uk
Supported by:
Project partially supported by the School of Pharmacy, University College London.

摘要/Abstract

摘要： Many studies have recently attempted to develop multifunctional nanoconstructs by integrating the superior fluorescence properties of quantum dots (QD) with therapeutic capabilities into a single vesicle for cancer theranostics. Liposome-quantum dot (L-QD) hybrid vesicles have shown promising potential for the construction of multifunctional nanoconstructs for cancer imaging and therapy. To fulfil such a potential, we report here the further functionalization of L-QD hybrid vesicles with therapeutic capabilities by loading anticancer drug doxorubicin (Dox) into their aqueous core. L-QD hybrid vesicles are first engineered by the incorporation of TOPO-capped, CdSe/ZnS QD into the lipid bilayers of DSPC:Chol:DSPE-PEG₂₀₀₀, followed by Dox loading using the pH-gradient technique. The loading efficiency of Dox into L-QD hybrid vesicles is achieved up to 97%, comparable to liposome control. All these evidences prove that the incorporation of QD into the lipid bilayer does not affect Dox loading through the lipid membrane of liposomes using the pH-gradient technique. Moreover, the release study shows that Dox release profile can be modulated simply by changing lipid composition. In conclusion, the Dox-loaded L-QD hybrid vesicles presented here constitute a promising multifunctional nanoconstruct capable of transporting combinations of therapeutic and diagnostic modalities.

关键词: nanomedicine, theranostic, multimodality, nanotechnology

Abstract: Many studies have recently attempted to develop multifunctional nanoconstructs by integrating the superior fluorescence properties of quantum dots (QD) with therapeutic capabilities into a single vesicle for cancer theranostics. Liposome-quantum dot (L-QD) hybrid vesicles have shown promising potential for the construction of multifunctional nanoconstructs for cancer imaging and therapy. To fulfil such a potential, we report here the further functionalization of L-QD hybrid vesicles with therapeutic capabilities by loading anticancer drug doxorubicin (Dox) into their aqueous core. L-QD hybrid vesicles are first engineered by the incorporation of TOPO-capped, CdSe/ZnS QD into the lipid bilayers of DSPC:Chol:DSPE-PEG₂₀₀₀, followed by Dox loading using the pH-gradient technique. The loading efficiency of Dox into L-QD hybrid vesicles is achieved up to 97%, comparable to liposome control. All these evidences prove that the incorporation of QD into the lipid bilayer does not affect Dox loading through the lipid membrane of liposomes using the pH-gradient technique. Moreover, the release study shows that Dox release profile can be modulated simply by changing lipid composition. In conclusion, the Dox-loaded L-QD hybrid vesicles presented here constitute a promising multifunctional nanoconstruct capable of transporting combinations of therapeutic and diagnostic modalities.

Key words: nanomedicine, theranostic, multimodality, nanotechnology

中图分类号: (Quantum dots)

78.67.Hc

61.46.Df (Structure of nanocrystals and nanoparticles ("colloidal" quantum dots but not gate-isolated embedded quantum dots))

田博文, Wafa' T. Al-Jamal, Kostas Kostarelos. The engineering of doxorubicin-loaded liposome-quantum dot hybrids for cancer theranostics[J]. 中国物理B, 2014, 23(8): 87805-087805.

Bowen Tian (田博文), Wafa' T. Al-Jamal, Kostas Kostarelos. The engineering of doxorubicin-loaded liposome-quantum dot hybrids for cancer theranostics[J]. Chin. Phys. B, 2014, 23(8): 87805-087805.

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The engineering of doxorubicin-loaded liposome-quantum dot hybrids for cancer theranostics

The engineering of doxorubicin-loaded liposome-quantum dot hybrids for cancer theranostics

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