中国物理B ›› 2005, Vol. 14 ›› Issue (7): 1277-1281.doi: 10.1088/1009-1963/14/7/001

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Studies of the beam finding and targeting accuracy of the CAS-LIBB single-particle microbeam

王晓华, 王旭飞, 胡智文, 陈连运, 张俊, 詹福如, 李军, 陈斌, 许明亮, 吴李君, 王绍虎, 余增亮   

  1. Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics,Chinese Academy of Sciences,and the Key Laboratory of Ion Beam Bioengineering in Anhui Province, Hefei 230031, China
  • 收稿日期:2004-10-29 修回日期:2005-01-12 出版日期:2005-06-20 发布日期:2005-06-20
  • 基金资助:
    Project supported by the National Major Technologies R\&D Program of China during the 10th Five-Year Plan Period (Grant No 2001BA302B-01), the magnitude instrument developing project of Chinese Academy of Sciences, Ministry of Agriculture ``948'' Project, the National Science Foundation of China (Grant No 19875054), and the National Science Fund for Distinguished Young Scholars, China (Grant No 10225526)

Studies of the beam finding and targeting accuracy of the CAS-LIBB single-particle microbeam

Wang Xiao-Hua (王晓华), Wang Xu-Fei (王旭飞), Hu Zhi-Wen (胡智文), Chen Lian-Yun (陈连运), Zhang Jun (张俊), Zhan Fu-Ru (詹福如), Li Jun (李军), Chen Bin (陈斌), Xu Ming-Liang (许明亮), Wu Li-Jun (吴李君), Wang Shao-Hu (王绍虎), Yu Zeng-Liang (余增亮)   

  1. Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics,Chinese Academy of Sciences,and the Key Laboratory of Ion Beam Bioengineering in Anhui Province, Hefei 230031, China
  • Received:2004-10-29 Revised:2005-01-12 Online:2005-06-20 Published:2005-06-20
  • Supported by:
    Project supported by the National Major Technologies R\&D Program of China during the 10th Five-Year Plan Period (Grant No 2001BA302B-01), the magnitude instrument developing project of Chinese Academy of Sciences, Ministry of Agriculture ``948'' Project, the National Science Foundation of China (Grant No 19875054), and the National Science Fund for Distinguished Young Scholars, China (Grant No 10225526)

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摘要： A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined number of hydrogen ions produced by a Van de Graaff accelerator, in an energy range of 2.0--3.0MeV,into an area smaller than the nuclei of individual living cells grown on thin plastic films. The beam is collimated by a borosilicate glass capillary that forms the beam-line exit. An computer integrated control program is developed to recognize the cells and to target them one by one for irradiation.Experiments for finding (capturing and recognizing) the microbeam position in the microscope imaging system and measuring the overall targeting accuracy of the facility are presented in this article. When a borosilicate glass capillary with 5$\mu$m inner diameter and 980$\mu$m length is used as the microbeam collimator, the overall targeting accuracy is that 91{\%} aimed pit clusters are located within 2.4 $\mu $m radius, and 98{\%} are within 3.6 $\mu $m radius.

关键词: single-particle microbeam, borosilicate glass capillary, CR-39, targeting accuracy

Abstract: A single-particle microbeam facility has been constructed at the Key Laboratory of Ion Beam Bioengineering (LIBB), Chinese Academy of Sciences (CAS). The system was designed to deliver a defined number of hydrogen ions produced by a Van de Graaff accelerator, in an energy range of 2.0--3.0MeV,into an area smaller than the nuclei of individual living cells grown on thin plastic films. The beam is collimated by a borosilicate glass capillary that forms the beam-line exit. An computer integrated control program is developed to recognize the cells and to target them one by one for irradiation.Experiments for finding (capturing and recognizing) the microbeam position in the microscope imaging system and measuring the overall targeting accuracy of the facility are presented in this article. When a borosilicate glass capillary with 5$\mu$m inner diameter and 980$\mu$m length is used as the microbeam collimator, the overall targeting accuracy is that 91% aimed pit clusters are located within 2.4 $\mu $m radius, and 98% are within 3.6 $\mu $m radius.

Key words: single-particle microbeam, borosilicate glass capillary, CR-39, targeting accuracy

中图分类号:  (Biophysical techniques (research methods))

  • 87.80.-y
87.17.Ee (Growth and division) 87.53.-j (Effects of ionizing radiation on biological systems) 29.20.-c (Accelerators)