中国物理B ›› 2006, Vol. 15 ›› Issue (4): 659-664.doi: 10.1088/1009-1963/15/4/001

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Elaborate calibration procedure for cell irradiation at the CAS-LIBB single-particle microbeam

余量登1, 丁克俭2, 张俊2, 吴李君2, 余增亮2, 胡智文3   

  1. (1)Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand; (2)Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China; (3)Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China;Department of Computer Science and Technology, Huaibei Coal Industry Teachers College, Huaibei 235000, China
  • 收稿日期:2005-09-08 修回日期:2006-01-17 出版日期:2006-04-20 发布日期:2006-04-20
  • 基金资助:
    Project supported by the National Major Technology Research and Development Program of China during the 10th Five-Year Plan Period (Grant No 2001BA302B), the National Science Fund for Distinguished Young Scholars of China (Grant No 10225526),the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No KSCX2-SW-324), and the Foundation for University Key Teacher by the Ministry of Education, China (Grant No 2005jq135).

Elaborate calibration procedure for cell irradiation at the CAS-LIBB single-particle microbeam

Hu Zhi-Wen (胡智文)ab, Ding Ke-Jian (丁克俭)a, Yu Liang-Deng (余量登)c, Zhang Jun (张俊)a, Wu Li-Jun (吴李君)a, Yu Zeng-Liang (余增亮)a    

  1. a Key Laboratory of Ion Beam Bioengineering, Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China; b Department of Computer Science and Technology, Huaibei Coal Industry Teachers College, Huaibei 235000, China; c Department of Physics, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand
  • Received:2005-09-08 Revised:2006-01-17 Online:2006-04-20 Published:2006-04-20
  • Supported by:
    Project supported by the National Major Technology Research and Development Program of China during the 10th Five-Year Plan Period (Grant No 2001BA302B), the National Science Fund for Distinguished Young Scholars of China (Grant No 10225526),the Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No KSCX2-SW-324), and the Foundation for University Key Teacher by the Ministry of Education, China (Grant No 2005jq135).

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摘要： Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro. It is crucial to find a reference point that relates the microbeam's location to the microscope's plane, and align individual targets at this reference point for cell irradiation. To choose an appropriate reference point, an approach based on analysing the intensity distribution of fluorescence in a thin scintillator excited by traversing particles is newly developed using the CAS-LIBB single-particle microbeam,which features decisive physical signification and sufficient resolution. As its bonus, this on-line analysis provides precise and fast response to the determination of beam profile and potentially optimizes the microbeam quality by further adjusting hardware setup.

关键词: single-particle microbeam, calibration procedure, cell irradiation, targeting accuracy

Abstract: Single-particle microbeam is uniquely capable of precisely delivering a preset number of charged particles to individual cells or sub-cellular targets to be determined in vitro. It is crucial to find a reference point that relates the microbeam's location to the microscope's plane, and align individual targets at this reference point for cell irradiation. To choose an appropriate reference point, an approach based on analysing the intensity distribution of fluorescence in a thin scintillator excited by traversing particles is newly developed using the CAS-LIBB single-particle microbeam,which features decisive physical signification and sufficient resolution. As its bonus, this on-line analysis provides precise and fast response to the determination of beam profile and potentially optimizes the microbeam quality by further adjusting hardware setup.

Key words: single-particle microbeam, calibration procedure, cell irradiation, targeting accuracy

中图分类号:  (Effects of ionizing radiation on biological systems)

  • 87.53.-j
06.20.fb (Standards and calibration) 07.77.Ka (Charged-particle beam sources and detectors) 87.80.-y (Biophysical techniques (research methods))