Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin

doi:10.1088/1009-1963/14/1/001

中国物理B ›› 2005, Vol. 14 ›› Issue (1): 1-5.doi: 10.1088/1009-1963/14/1/001

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Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin

李方华¹, 王怀斌¹, 古元新¹, 范海福¹, 阳世新², 刘玉东³

(1)Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, China; (2)College of Life Science, Sichuan University, Chengdu 610064, China; (3)Lineberger Comprehensive Cancer Centre, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA

收稿日期:2004-07-06 修回日期:2004-08-13 出版日期:2005-01-20 发布日期:2005-01-20
基金资助:
Natural Science Foundation of China (Grant Nos 19574073 and19634020)

Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin

Yang Shi-Xin (阳世新)^a, Li Fang-Hua (李方华)^b, Liu Yu-Dong (刘玉东)^c, Wang Huai-Bin (王怀斌)^b, Gu Yuan-Xin (古元新)^b, Fan Hai-Fu (范海福)^b

^a College of Life Science, Sichuan University, Chengdu 610064, China; ^b Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100080, China; ^c Lineberger Comprehensive Cancer Centre, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599-7295, USA

Received:2004-07-06 Revised:2004-08-13 Online:2005-01-20 Published:2005-01-20
Supported by:
Natural Science Foundation of China (Grant Nos 19574073 and19634020)

摘要/Abstract

摘要： Direct-method phase extension has been applied to two-dimensional electrondiffraction data of the protein streptavidin. Structure-factor amplitudesfrom electron diffraction were combined with phases from the correspondingelectron micrographs. Maximum-entropy discrimination and cluster analysiswere used to derive a solution from a large number of random trials. The phase extension from 0.3 to 0.25nm led to substantial improvement of thereconstructed projection image quality.

关键词: electron crystallography, direct method, protein structure, streptavidin

Abstract: Direct-method phase extension has been applied to two-dimensional electrondiffraction data of the protein streptavidin. Structure-factor amplitudesfrom electron diffraction were combined with phases from the correspondingelectron micrographs. Maximum-entropy discrimination and cluster analysiswere used to derive a solution from a large number of random trials. The phase extension from 0.3 to 0.25nm led to substantial improvement of thereconstructed projection image quality.

Key words: electron crystallography, direct method, protein structure, streptavidin

中图分类号:

0290

阳世新, 李方华, 刘玉东, 王怀斌, 古元新, 范海福. Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin[J]. 中国物理B, 2005, 14(1): 1-5.

Yang Shi-Xin (阳世新), Li Fang-Hua (李方华), Liu Yu-Dong (刘玉东), Wang Huai-Bin (王怀斌), Gu Yuan-Xin (古元新), Fan Hai-Fu (范海福). Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin[J]. Chinese Physics, 2005, 14(1): 1-5.

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Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin

Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin

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