Structural modeling of proteins by integrating small-angle x-ray scattering data

doi:10.1088/1674-1056/24/12/126101

中国物理B ›› 2015, Vol. 24 ›› Issue (12): 126101-126101.doi: 10.1088/1674-1056/24/12/126101

所属专题： TOPICAL REVIEW — 8th IUPAP International Conference on Biological Physics

• TOPICAL REVIEW—8th IUPAP International Conference on Biological Physics • 上一篇下一篇

Structural modeling of proteins by integrating small-angle x-ray scattering data

张泳辉, 彭俊辉, 张志勇

Hefei National Laboratory for Physical Science at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230026, China

收稿日期:2015-04-29 修回日期:2015-07-14 出版日期:2015-12-05 发布日期:2015-12-05
通讯作者: Zhang Zhi-Yong E-mail:zzyzhang@ustc.edu.cn
基金资助:
Project supported by the National Key Basic Research Program of China (Grant Nos. 2013CB910203 and 2011CB911104), the National Natural Science Foundation of China (Grant No. 31270760), the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB08030102), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20113402120013).

Structural modeling of proteins by integrating small-angle x-ray scattering data

Zhang Yong-Hui (张泳辉), Peng Jun-Hui (彭俊辉), Zhang Zhi-Yong (张志勇)

Hefei National Laboratory for Physical Science at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230026, China

Received:2015-04-29 Revised:2015-07-14 Online:2015-12-05 Published:2015-12-05
Contact: Zhang Zhi-Yong E-mail:zzyzhang@ustc.edu.cn
Supported by:
Project supported by the National Key Basic Research Program of China (Grant Nos. 2013CB910203 and 2011CB911104), the National Natural Science Foundation of China (Grant No. 31270760), the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB08030102), and the Specialized Research Fund for the Doctoral Program of Higher Education of China (Grant No. 20113402120013).

摘要/Abstract

摘要：

Elucidating the structure of large biomolecules such as multi-domain proteins or protein complexes is challenging due to their high flexibility in solution. Recently, an “integrative structural biology” approach has been proposed, which aims to determine the protein structure and characterize protein flexibility by combining complementary high-and low-resolution experimental data using computer simulations. Small-angle x-ray scattering (SAXS) is an efficient technique that can yield low-resolution structural information, including protein size and shape. Here, we review computational methods that integrate SAXS with other experimental datasets for structural modeling. Finally, we provide a case study of determination of the structure of a protein complex formed between the tandem SH3 domains in c-Cb1-associated protein and the proline-rich loop in human vinculin.

关键词: protein structure, flexibility, computer simulations, small-angle x-ray scattering, integrative modeling

Abstract:

Key words: protein structure, flexibility, computer simulations, small-angle x-ray scattering, integrative modeling

中图分类号: (X-ray scattering (including small-angle scattering))

61.05.cf

87.14.E- (Proteins) 87.15.ap (Molecular dynamics simulation)

张泳辉, 彭俊辉, 张志勇. Structural modeling of proteins by integrating small-angle x-ray scattering data[J]. 中国物理B, 2015, 24(12): 126101-126101.

Zhang Yong-Hui (张泳辉), Peng Jun-Hui (彭俊辉), Zhang Zhi-Yong (张志勇). Structural modeling of proteins by integrating small-angle x-ray scattering data[J]. Chin. Phys. B, 2015, 24(12): 126101-126101.

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Structural modeling of proteins by integrating small-angle x-ray scattering data

Structural modeling of proteins by integrating small-angle x-ray scattering data

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