Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel

doi:10.1088/1674-1056/acfaf6

中国物理B ›› 2023, Vol. 32 ›› Issue (11): 118704-118704.doi: 10.1088/1674-1056/acfaf6

所属专题： SPECIAL TOPIC — Celebrating the 100th Anniversary of Physics Discipline of Northwest University

Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel

Jie Zhu(朱洁), Jing Xue(薛菁), Wei Zhao(赵伟), Chen Zhang(张琛), Xiaoqiang Feng(冯晓强), and Kaige Wang(王凯歌)^†

State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Key Laboratory of Optoelectronic Technology of Shaanxi Province, National Center for International Research of Photoelectric Technology & Nano-functional Materials and Application, Institute of Photonics and Photon-Technology, Northwest University, Xi'an 710069, China

收稿日期:2023-06-30 修回日期:2023-08-28 接受日期:2023-09-19 出版日期:2023-10-16 发布日期:2023-11-07
通讯作者: Kaige Wang E-mail:wangkg@nwu.edu.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant Nos. 62275216 and 61775181), the Innovation Capability Support Program of Shaanxi Province of China (Grant Nos. S2018-ZC-TD-0061 and TZ0393), and the National Key Scientific Instrument and Equipment Development Projects of China (Grant No. 51927804).

Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel

Jie Zhu(朱洁), Jing Xue(薛菁), Wei Zhao(赵伟), Chen Zhang(张琛), Xiaoqiang Feng(冯晓强), and Kaige Wang(王凯歌)^†

State Key Laboratory of Cultivation Base for Photoelectric Technology and Functional Materials, Key Laboratory of Optoelectronic Technology of Shaanxi Province, National Center for International Research of Photoelectric Technology & Nano-functional Materials and Application, Institute of Photonics and Photon-Technology, Northwest University, Xi'an 710069, China

Received:2023-06-30 Revised:2023-08-28 Accepted:2023-09-19 Online:2023-10-16 Published:2023-11-07
Contact: Kaige Wang E-mail:wangkg@nwu.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant Nos. 62275216 and 61775181), the Innovation Capability Support Program of Shaanxi Province of China (Grant Nos. S2018-ZC-TD-0061 and TZ0393), and the National Key Scientific Instrument and Equipment Development Projects of China (Grant No. 51927804).

摘要/Abstract

摘要： Interactions between deoxyribonucleic acid (DNA) and metal ions are vital for maintaining life functions, however, there are still unsolved questions about its mechanisms. It is of great practical significance to study these issues for medical chip design, drug development, health care, etc. In this investigation, the conductivity properties of λ -DNA solutions with mono-/divalent metal ions (Na⁺, K⁺, Mg²⁺, and Ca²⁺) are experimentally studied as they are electrically driven through a 5 μ m microfluidic channel. Experimental data indicate that the conductivities of λ -DNA solutions with metal ions (M⁺/M²⁺) basically tend to reduce firstly and then increase as the voltage increases, of which the turning points varied with the metal ions. When the voltage surpasses turning points, the conductivity of λ -DNA-M⁺ solutions increases with the concentration of metal ions, while that of λ -DNA-M²⁺ solutions decrease. Moreover, the conductivity of λ -DNA-M²⁺ solutions is always smaller than that of λ -DNA-M⁺ solutions, and with high-concentration M²⁺, it is even smaller than that of the λ -DNA solution. The main reasons for the above findings could be attributed to the polarization of electrodes and different mechanisms of interactions between metal ions and λ -DNA molecules. This investigation is helpful for the precise manipulation of single DNA molecules in micro-/nanofluidic space and the design of new biomedical micro-/nanofluidic sensors.

关键词: microfluidics, interaction between deoxyribonucleic acid (DNA) and metal ions, conductivity-voltage relations, asymmetric electrodes

Abstract: Interactions between deoxyribonucleic acid (DNA) and metal ions are vital for maintaining life functions, however, there are still unsolved questions about its mechanisms. It is of great practical significance to study these issues for medical chip design, drug development, health care, etc. In this investigation, the conductivity properties of λ -DNA solutions with mono-/divalent metal ions (Na⁺, K⁺, Mg²⁺, and Ca²⁺) are experimentally studied as they are electrically driven through a 5 μ m microfluidic channel. Experimental data indicate that the conductivities of λ -DNA solutions with metal ions (M⁺/M²⁺) basically tend to reduce firstly and then increase as the voltage increases, of which the turning points varied with the metal ions. When the voltage surpasses turning points, the conductivity of λ -DNA-M⁺ solutions increases with the concentration of metal ions, while that of λ -DNA-M²⁺ solutions decrease. Moreover, the conductivity of λ -DNA-M²⁺ solutions is always smaller than that of λ -DNA-M⁺ solutions, and with high-concentration M²⁺, it is even smaller than that of the λ -DNA solution. The main reasons for the above findings could be attributed to the polarization of electrodes and different mechanisms of interactions between metal ions and λ -DNA molecules. This investigation is helpful for the precise manipulation of single DNA molecules in micro-/nanofluidic space and the design of new biomedical micro-/nanofluidic sensors.

Key words: microfluidics, interaction between deoxyribonucleic acid (DNA) and metal ions, conductivity-voltage relations, asymmetric electrodes

中图分类号: (DNA)

87.14.gk

87.19.rh (Fluid transport and rheology) 47.27.nd (Channel flow) 87.15.H- (Dynamics of biomolecules)

Jie Zhu(朱洁), Jing Xue(薛菁), Wei Zhao(赵伟), Chen Zhang(张琛), Xiaoqiang Feng(冯晓强), and Kaige Wang(王凯歌). Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel[J]. 中国物理B, 2023, 32(11): 118704-118704.

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Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel

Effect of mono-/divalent metal ions on the conductivity characteristics of DNA solutions transferring through a microfluidic channel

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