Stochastic fluctuations in the rotation of the bacterial flagellar motor

doi:10.1088/1674-1056/acc804

中国物理B ›› 2023, Vol. 32 ›› Issue (6): 68703-068703.doi: 10.1088/1674-1056/acc804

Stochastic fluctuations in the rotation of the bacterial flagellar motor

Guanhua Yue(岳冠骅), Rongjing Zhang(张榕京)^†, and Junhua Yuan(袁军华)^‡

Department of Physics, University of Science and Technology of China, Hefei 230026, China

收稿日期:2023-02-01 修回日期:2023-03-16 接受日期:2023-03-28 出版日期:2023-05-17 发布日期:2023-05-22
通讯作者: Rongjing Zhang, Junhua Yuan E-mail:rjzhang@ustc.edu.cn;jhyuan@ustc.edu.cn
基金资助:
Project supported by the National Natural Science Foundation of China (Grant Nos. 11925406 and 12090053) and the National Key R&D Program of China (Grant No. 2019YFA0709303).

Stochastic fluctuations in the rotation of the bacterial flagellar motor

Guanhua Yue(岳冠骅), Rongjing Zhang(张榕京)^†, and Junhua Yuan(袁军华)^‡

Department of Physics, University of Science and Technology of China, Hefei 230026, China

Received:2023-02-01 Revised:2023-03-16 Accepted:2023-03-28 Online:2023-05-17 Published:2023-05-22
Contact: Rongjing Zhang, Junhua Yuan E-mail:rjzhang@ustc.edu.cn;jhyuan@ustc.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Grant Nos. 11925406 and 12090053) and the National Key R&D Program of China (Grant No. 2019YFA0709303).

1. 2023-068703-SI.docx(740KB)

摘要/Abstract

摘要： The bacterial flagellar motor is a nanometer-sized rotary motor that generates the torque to drive the rotation of the flagellar filament. The output torque is an important property of the motor. The motor rotation was usually monitored by attaching a μm-sized bead to a shortened flagellar filament, and the torque was extracted by calculating the torque due to the viscous drag of the medium on the bead rotation. We sought for an independent extraction of the torque from thermal fluctuation in the motor rotation using the fluctuation theorem (FT). However, we identified an overwhelming fluctuation beyond the thermal noise that precluded the use of FT. We further characterized the timescale and the amplitude of this fluctuation, finding that it was probably due to the stepping of the motor. The amplitude of torque fluctuation we characterized here provided new information on the torque-generating interaction potential curve.

关键词: motor torque, bacterial motility, fluctuation theorem, viscous drag

Abstract: The bacterial flagellar motor is a nanometer-sized rotary motor that generates the torque to drive the rotation of the flagellar filament. The output torque is an important property of the motor. The motor rotation was usually monitored by attaching a μm-sized bead to a shortened flagellar filament, and the torque was extracted by calculating the torque due to the viscous drag of the medium on the bead rotation. We sought for an independent extraction of the torque from thermal fluctuation in the motor rotation using the fluctuation theorem (FT). However, we identified an overwhelming fluctuation beyond the thermal noise that precluded the use of FT. We further characterized the timescale and the amplitude of this fluctuation, finding that it was probably due to the stepping of the motor. The amplitude of torque fluctuation we characterized here provided new information on the torque-generating interaction potential curve.

Key words: motor torque, bacterial motility, fluctuation theorem, viscous drag

中图分类号: (Movement and locomotion)

87.85.gj

87.15.Ya (Fluctuations) 87.17.Jj (Cell locomotion, chemotaxis) 05.10.Gg (Stochastic analysis methods)

Guanhua Yue(岳冠骅), Rongjing Zhang(张榕京), and Junhua Yuan(袁军华). Stochastic fluctuations in the rotation of the bacterial flagellar motor[J]. 中国物理B, 2023, 32(6): 68703-068703.

Guanhua Yue(岳冠骅), Rongjing Zhang(张榕京), and Junhua Yuan(袁军华). Stochastic fluctuations in the rotation of the bacterial flagellar motor[J]. Chin. Phys. B, 2023, 32(6): 68703-068703.

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Stochastic fluctuations in the rotation of the bacterial flagellar motor

Stochastic fluctuations in the rotation of the bacterial flagellar motor

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